| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,530,076 | (A)7→8 | coding (155/483 nt) | ydcD → | putative immunity protein for RhsE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,530,069 | 1 | . | A | 91.5% | 192.5 / 10.8 | 59 | coding (148/483 nt) | ydcD | putative immunity protein for RhsE |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base A (27/27); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑AAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGG > NC_000913/1529975‑1530158 | aTTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑aaa‑aa > 2:909908/1‑100 (MQ=255) tttGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑aaa‑aaa < 2:604559/100‑1 (MQ=255) tgttgtTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑aaa‑aaaaa > 2:129766/1‑99 (MQ=255) gttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑aaa‑aaaaatatt > 1:107037/1‑95 (MQ=255) gttTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑aaa‑aaaaatatt > 1:1134913/1‑95 (MQ=255) ttACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAAtattat > 2:489147/1‑100 (MQ=255) tACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATattatt > 1:261837/1‑100 (MQ=255) aCCTTGGGTTTGATAACAATTCAAATTCTGACATCTTATTTGATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATAttattt > 2:711178/1‑100 (MQ=255) ccTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATAttattta > 2:27231/1‑100 (MQ=255) ccTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATAttattta > 2:348055/1‑100 (MQ=255) ccTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATAttattta > 1:498075/1‑100 (MQ=255) cTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATAttatttat < 2:828314/100‑1 (MQ=255) ttGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTtata > 1:1187119/1‑100 (MQ=255) tGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAaataat > 1:898340/1‑100 (MQ=255) gATAAAAAATCAAATTCTGACATCATATATTAAAAATAAAAAACACAAAAGAGCGTTGCGATAAAAAAAAAAA‑A‑AAA‑AAAATATTATTTATAAAAtaatt < 2:713062/100‑1 (MQ=255) aTAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAAtaatta > 2:442098/1‑100 (MQ=255) aTAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAAtaatta < 2:656723/100‑1 (MQ=255) aTAACAATTCAAATTCTGACATCGTATTTTACGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAAtaatta < 1:434939/100‑1 (MQ=255) tAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAAtaattaa > 1:529897/1‑100 (MQ=255) tAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAAtaattaa > 1:1098519/1‑100 (MQ=255) tAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAAtaattaa > 2:1034654/1‑100 (MQ=255) aCAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATg < 2:444648/100‑1 (MQ=255) aTTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGAcc < 1:754784/100‑1 (MQ=255) ttCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCa > 2:356203/1‑100 (MQ=255) tCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAAtt < 1:361656/100‑1 (MQ=255) cTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAAtt < 2:975056/99‑1 (MQ=255) tGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATtag < 1:742044/100‑1 (MQ=255) tGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATtag < 1:728424/100‑1 (MQ=255) tGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATtag < 1:1117828/100‑1 (MQ=255) acataaaaatttATGGACATAAAAAACCAAAGAGCGTTGAAAAATAACTTTA‑A‑AAAAA‑AAAATATTATTTATAAAATAATAAAAAAAAAAAAAAtt < 2:1168042/87‑1 (MQ=255) aCATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATtagta < 1:129768/100‑1 (MQ=255) aTCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTaga > 1:232559/1‑100 (MQ=255) aTCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTaga > 2:240842/1‑100 (MQ=255) aTCGTATTTTATAGACATAAAACACCAAAGAGCATAGAAATATATCTTTC‑TAA‑AAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAAt < 2:18277/93‑1 (MQ=255) tCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTagag > 2:686740/1‑100 (MQ=255) gTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAggg > 2:645124/1‑100 (MQ=255) aTTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑AAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAAt < 1:766796/100‑1 (MQ=255) tttAAGAACATAAAACACCAAAGAGCATTGAGATATATCTTTA‑T‑GAAAA‑AAAATATTATTTATAAACTAATTAATGCCCCCCAAACTAATAGAGGGCATg < 2:261834/100‑1 (MQ=255) tATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGt < 2:529893/100‑1 (MQ=255) gACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt > 1:1261555/1‑100 (MQ=255) gACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt < 1:1107179/100‑1 (MQ=255) gACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtt > 2:599040/1‑100 (MQ=255) aCATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAttt > 1:752161/1‑100 (MQ=255) aCATAAAACACCAAAGAGCGTTGAGATATAACATTA‑A‑AAAAA‑AAAATATTATTTATAAAATAAATAATAAAAAAAAAATTAGTAGAGGGAATGGTCa < 2:31517/97‑1 (MQ=255) cATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCAtttt < 1:240844/100‑1 (MQ=255) aTAAAACACCAAAGAGCGTTGAGATATAAAAAAA‑A‑AAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTta < 2:664382/100‑1 (MQ=255) tAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTtat > 1:535412/1‑100 (MQ=255) aaaaCACCAAAGAGCGTTGAGACATAAATTTC‑T‑G‑AAAGAAAATATTACTTATAAAATAATTAATAAAAAAAAAATTAGTAGAGGGAATGGTCATTTtata < 2:879383/100‑1 (MQ=255) aaaCACCAAAGAGCGTTGAGATATATCCTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATaa < 2:370619/100‑1 (MQ=255) aaCACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg < 1:450002/100‑1 (MQ=255) aaCACCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg < 1:57091/100‑1 (MQ=255) aaCACCAAAAAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAg < 2:870249/100‑1 (MQ=255) acacCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATGTTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGt < 2:675678/100‑1 (MQ=255) acacCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGt > 1:597793/1‑100 (MQ=255) acCAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGtat < 2:129306/100‑1 (MQ=255) cAAAGAGCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATaa > 2:375150/1‑100 (MQ=255) agCGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGtt > 1:838457/1‑100 (MQ=255) cGTTGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaa < 1:405551/100‑1 (MQ=255) ttGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAACATAATTCATCACCAAAAAATTAGTTTTGGGAAAGTTAATTATATAAATATCATGCTtaata > 2:643172/1‑100 (MQ=255) ttGAGATATATCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaata > 2:739815/1‑100 (MQ=255) ttGAGATATATCTTTC‑T‑G‑AAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTtaataa > 1:974528/1‑100 (MQ=255) tatatCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACa < 1:1049957/100‑1 (MQ=255) atatCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAg < 1:686744/100‑1 (MQ=255) tatCTTTC‑T‑GAAAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAgg > 1:527729/1‑100 (MQ=255) | ATTTGTTGTTTACCTTGGGTTTGATAACAATTCAAATTCTGACATCGTATTTTATGGACATAAAACACCAAAGAGCGTTGAGATATATCTTTC‑T‑G‑AAA‑AAAATATTATTTATAAAATAATTAATGACCAAAAAATTAGTAGAGGGAATGGTCATTTTATAAGTATAATGGTTAATAATTACAGG > NC_000913/1529975‑1530158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |