Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,642,062 +T coding (208/213 nt) cspF → Qin prophage; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,642,0591.T95.7% 161.4 / 0.7 47coding (205/213 nt)cspFQin prophage; cold shock protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (24/21);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTG  >  NC_000913/1641960‑1642154
                                                                                                    |                                                                                                
gcgcTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTAcc                                                                                                   <  2:44834/100‑1 (MQ=255)
gcgcTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTAcc                                                                                                   <  2:604731/100‑1 (MQ=255)
 cgcTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCt                                                                                                 >  2:1125904/1‑100 (MQ=255)
  gcTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCtt                                                                                               >  1:563259/1‑100 (MQ=255)
  gcTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCtt                                                                                               >  1:12573/1‑100 (MQ=255)
   cTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttt                                                                                              <  2:872239/100‑1 (MQ=255)
   cTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttt                                                                                              >  2:378674/1‑100 (MQ=255)
     cAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttc                                                                                            >  2:1019152/1‑98 (MQ=255)
     cAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttc                                                                                            >  2:209683/1‑98 (MQ=255)
     cAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttc                                                                                            <  2:636424/100‑3 (MQ=255)
      aaTCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttca                                                                                           <  1:81190/100‑4 (MQ=255)
      aaTCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCttttca                                                                                           >  2:572067/1‑97 (MQ=255)
          tcCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAg                                                                                        <  2:367906/100‑1 (MQ=255)
           ccGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGc                                                                                       >  1:411595/1‑100 (MQ=255)
           ccGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGc                                                                                       <  1:719099/100‑1 (MQ=255)
            cgcgATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCt                                                                                      >  1:64793/1‑100 (MQ=255)
             gcgATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCta                                                                                     >  1:42996/1‑100 (MQ=255)
                   cAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTaa                                                                               >  2:17005/1‑100 (MQ=255)
                     gaagaaATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAg                                                                             >  1:1001138/1‑100 (MQ=255)
                     gaagaaATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAg                                                                             >  1:1116210/1‑100 (MQ=255)
                      aagaaATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTGTTCATGAGCTATATTAAAGc                                                                            <  1:1226367/100‑1 (MQ=255)
                         aaaTTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCttt                                                                         <  1:267169/100‑1 (MQ=255)
                            ttACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAAt                                                                      >  2:739737/1‑100 (MQ=255)
                                   cGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGc                                                               <  2:1004781/100‑1 (MQ=255)
                                     gATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGccc                                                             >  1:103970/1‑100 (MQ=255)
                                      aTTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGcccc                                                            >  1:865931/1‑100 (MQ=255)
                                           gcgTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCg                                                        <  2:453043/99‑1 (MQ=255)
                                            cgTGGAATTTTGTCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGa                                                      >  1:856891/1‑100 (MQ=255)
                                             gTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCTGGCCCCATCGGAt                                                     >  2:821441/1‑100 (MQ=255)
                                              tGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATc                                                    >  2:526613/1‑100 (MQ=255)
                                              tGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATc                                                    <  2:935885/100‑1 (MQ=255)
                                                  aTTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATcacac                                                <  2:462576/100‑1 (MQ=255)
                                                   ttttGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATcacaca                                               <  1:866051/100‑1 (MQ=255)
                                                      tGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATgg                                            <  1:915681/100‑1 (MQ=255)
                                                        ccGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGgag                                          <  1:964476/100‑1 (MQ=255)
                                                             tAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGttt                                     <  1:54826/100‑1 (MQ=255)
                                                               aaTGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGttttt                                   <  1:1125914/100‑1 (MQ=255)
                                                                aTGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTa                                  >  1:816998/1‑100 (MQ=255)
                                                                 tGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTAt                                 >  1:419165/1‑100 (MQ=255)
                                                                  ggTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATg                                <  1:395531/100‑1 (MQ=255)
                                                                    tCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGaa                              >  1:185172/1‑100 (MQ=255)
                                                                         gTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCATGCCCCATCGGATCACACATGGAGAGATTTTATTAATAAcc                         >  2:161970/1‑100 (MQ=255)
                                                                         gTGGCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAAcc                         <  1:739742/100‑1 (MQ=255)
                                                                            gCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCg                      <  2:411591/100‑1 (MQ=255)
                                                                            gCCCTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCg                      >  1:228550/1‑100 (MQ=255)
                                                                              ccTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtc                    >  1:669352/1‑100 (MQ=255)
                                                                              ccTTCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtc                    <  1:17005/100‑1 (MQ=255)
                                                                                ttCAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtctg                  <  2:865925/100‑1 (MQ=255)
                                                                                  cAGCTGCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCgtctgtc                >  2:667068/1‑100 (MQ=255)
                                                                                       gCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgat           <  2:164277/100‑1 (MQ=255)
                                                                                       gCCAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtg             >  1:272491/1‑98 (MQ=255)
                                                                                        ccAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgatg          >  1:206951/1‑100 (MQ=255)
                                                                                        ccAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgatg          >  1:1205441/1‑100 (MQ=255)
                                                                                        ccAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgatg          <  2:228548/100‑1 (MQ=255)
                                                                                         cAATGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTtgatga         >  2:316779/1‑100 (MQ=255)
                                                                                            tGTTTACCTTTTCATGAGCTATATTAAAGCTTTAATTTTAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGAc        >  2:482844/1‑98 (MQ=255)
                                                                                              tttACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGt    <  1:242413/100‑1 (MQ=255)
                                                                                               ttACCTTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGtt   <  1:1224375/100‑1 (MQ=255)
                                                                                               tcaCCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTg  <  2:792273/98‑1 (MQ=255)
                                                                                                    |                                                                                                
GCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTG  >  NC_000913/1641960‑1642154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: