Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,580,172 | 0 | T | C | 20.4% | 92.9 / 17.8 | 50 | G179G (GGT→GGC) | tktB | transketolase 2, thiamine triphosphate‑binding |
Reads supporting (aligned to +/- strand): ref base T (25/14); new base C (3/7); total (29/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.60e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 |
TTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATC > NC_000913/2580074‑2580271 | ttCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtt < 2:968637/100‑1 (MQ=255) tCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGCACGTATATCCAACGAAGTCTATTCGCTCGCAGGAAAGCTGGGACTGGGCAAGCTGATTGGttt < 1:1040139/100‑1 (MQ=255) cACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt > 2:924093/1‑100 (MQ=255) cACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt < 2:751546/100‑1 (MQ=255) cACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt > 1:400197/1‑100 (MQ=255) ccTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTa > 2:278780/1‑100 (MQ=255) ccTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTa > 2:495466/1‑100 (MQ=255) cTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTTGGGCTGGGCAAGCTGATTGGGTTTTAc > 2:320418/1‑100 (MQ=255) gtgtTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATc < 2:185815/100‑1 (MQ=255) gtgtTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATc > 2:720050/1‑100 (MQ=255) tATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACaa > 1:53103/1‑99 (MQ=255) tATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAAc > 1:549298/1‑100 (MQ=255) tATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAAc > 1:769192/1‑100 (MQ=255) tGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACgg > 1:196382/1‑100 (MQ=255) ggCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTa > 2:171877/1‑100 (MQ=255) cGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTcc > 2:1219991/1‑100 (MQ=255) cTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATc < 2:199937/100‑1 (MQ=255) cTGCCTGATGGAACGCATTTCCCACGAAGTCTGTGCGCTGGCAGGCACGCTGGGACTGTGCAAGCTGATTGGCTTTTACTATCACAACGGTATTTcc < 2:1193900/97‑1 (MQ=255) ccTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGAc > 1:806915/1‑100 (MQ=255) ccTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGAc > 2:1036021/1‑100 (MQ=255) cTGATGGAGGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACg > 1:14433/1‑100 (MQ=255) cTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACg < 1:262231/100‑1 (MQ=255) cTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACg > 1:1162808/1‑100 (MQ=255) aaGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAAc > 1:821855/1‑100 (MQ=255) aaGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAAc > 1:162784/1‑100 (MQ=255) tATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGaa < 1:677313/100‑1 (MQ=255) ttCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGc > 2:296283/1‑100 (MQ=255) tCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCt > 2:473181/1‑100 (MQ=255) cccACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTg > 1:256156/1‑100 (MQ=255) cccACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTg > 2:1051503/1‑100 (MQ=255) ccACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTgg > 1:16827/1‑100 (MQ=255) gAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTTCGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGttt < 2:727086/99‑1 (MQ=255) aGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGCAGGCTGGTTTAcc > 2:142427/1‑100 (MQ=255) aGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTAcc < 1:924101/100‑1 (MQ=255) tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc < 1:495470/100‑1 (MQ=255) tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc < 2:351379/100‑1 (MQ=255) tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc < 1:278783/100‑1 (MQ=255) tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAATTGTCTTTCCATCGACGGTGAAACCGAAGGCTGGTTTACCGACGATACGGc < 2:1192928/100‑1 (MQ=255) ggcaggcaCGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCa < 1:799736/100‑1 (MQ=255) caCGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACg > 1:979289/1‑99 (MQ=255) gCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGtttt < 2:565519/100‑1 (MQ=255) gCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGtttt < 1:1149867/100‑1 (MQ=255) gCTGGGAATGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGtttt < 2:432995/100‑1 (MQ=255) gggACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGaa < 1:356723/100‑1 (MQ=255) ggACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAg < 2:979281/100‑1 (MQ=255) gACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGGCGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGc < 1:41657/100‑1 (MQ=255) gACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGc < 1:940228/100‑1 (MQ=255) cTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCt > 2:147008/1‑100 (MQ=255) gggCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTAt > 2:286552/1‑100 (MQ=255) gCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCa < 2:144765/100‑1 (MQ=255) cAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCAc > 2:604553/1‑100 (MQ=255) tGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTGCCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGc > 2:381677/1‑99 (MQ=255) aTTGGTTTTTACGATCAAAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCAtg > 2:1238223/1‑100 (MQ=255) tGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCAtgtg < 1:1036031/100‑1 (MQ=255) ggTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGa > 2:235811/1‑100 (MQ=255) tttttACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATc < 2:549294/100‑1 (MQ=255) | TTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATC > NC_000913/2580074‑2580271 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |