Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,580,1720TC20.4% 92.9 / 17.8 50G179G (GGT→GGCtktBtransketolase 2, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (25/14);  new base C (3/7);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 7.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

TTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATC  >  NC_000913/2580074‑2580271
                                                                                                  |                                                                                                   
ttCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtt                                                                                                    <  2:968637/100‑1 (MQ=255)
 tCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGCACGTATATCCAACGAAGTCTATTCGCTCGCAGGAAAGCTGGGACTGGGCAAGCTGATTGGttt                                                                                                   <  1:1040139/100‑1 (MQ=255)
  cACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt                                                                                                  >  2:924093/1‑100 (MQ=255)
  cACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt                                                                                                  <  2:751546/100‑1 (MQ=255)
  cACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGtttt                                                                                                  >  1:400197/1‑100 (MQ=255)
    ccTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTa                                                                                                >  2:278780/1‑100 (MQ=255)
    ccTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTa                                                                                                >  2:495466/1‑100 (MQ=255)
     cTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTTGGGCTGGGCAAGCTGATTGGGTTTTAc                                                                                               >  2:320418/1‑100 (MQ=255)
         gtgtTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATc                                                                                           <  2:185815/100‑1 (MQ=255)
         gtgtTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATc                                                                                           >  2:720050/1‑100 (MQ=255)
              tATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACaa                                                                                       >  1:53103/1‑99 (MQ=255)
              tATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAAc                                                                                      >  1:549298/1‑100 (MQ=255)
              tATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAAc                                                                                      >  1:769192/1‑100 (MQ=255)
                tGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACgg                                                                                    >  1:196382/1‑100 (MQ=255)
                  ggCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTa                                                                                  >  2:171877/1‑100 (MQ=255)
                       cGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTcc                                                                             >  2:1219991/1‑100 (MQ=255)
                          cTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATc                                                                          <  2:199937/100‑1 (MQ=255)
                          cTGCCTGATGGAACGCATTTCCCACGAAGTCTGTGCGCTGGCAGGCACGCTGGGACTGTGCAAGCTGATTGGCTTTTACTATCACAACGGTATTTcc                                                                             <  2:1193900/97‑1 (MQ=255)
                             ccTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGAc                                                                       >  1:806915/1‑100 (MQ=255)
                             ccTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGAc                                                                       >  2:1036021/1‑100 (MQ=255)
                              cTGATGGAGGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACg                                                                      >  1:14433/1‑100 (MQ=255)
                              cTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACg                                                                      <  1:262231/100‑1 (MQ=255)
                              cTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACg                                                                      >  1:1162808/1‑100 (MQ=255)
                                     aaGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAAc                                                               >  1:821855/1‑100 (MQ=255)
                                     aaGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAAc                                                               >  1:162784/1‑100 (MQ=255)
                                         tATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGaa                                                           <  1:677313/100‑1 (MQ=255)
                                            ttCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGc                                                        >  2:296283/1‑100 (MQ=255)
                                             tCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCt                                                       >  2:473181/1‑100 (MQ=255)
                                              cccACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTg                                                      >  1:256156/1‑100 (MQ=255)
                                              cccACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTg                                                      >  2:1051503/1‑100 (MQ=255)
                                               ccACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTgg                                                     >  1:16827/1‑100 (MQ=255)
                                                   gAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTTCGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGttt                                                  <  2:727086/99‑1 (MQ=255)
                                                     aGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGCAGGCTGGTTTAcc                                               >  2:142427/1‑100 (MQ=255)
                                                     aGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTAcc                                               <  1:924101/100‑1 (MQ=255)
                                                                tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc                                    <  1:495470/100‑1 (MQ=255)
                                                                tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc                                    <  2:351379/100‑1 (MQ=255)
                                                                tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc                                    <  1:278783/100‑1 (MQ=255)
                                                                tGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAATTGTCTTTCCATCGACGGTGAAACCGAAGGCTGGTTTACCGACGATACGGc                                    <  2:1192928/100‑1 (MQ=255)
                                                                 ggcaggcaCGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCa                                   <  1:799736/100‑1 (MQ=255)
                                                                       caCGCTGGGACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACg                              >  1:979289/1‑99 (MQ=255)
                                                                          gCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGtttt                          <  2:565519/100‑1 (MQ=255)
                                                                          gCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGtttt                          <  1:1149867/100‑1 (MQ=255)
                                                                          gCTGGGAATGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGtttt                          <  2:432995/100‑1 (MQ=255)
                                                                             gggACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGaa                       <  1:356723/100‑1 (MQ=255)
                                                                              ggACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAg                      <  2:979281/100‑1 (MQ=255)
                                                                               gACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGGCGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGc                     <  1:41657/100‑1 (MQ=255)
                                                                               gACTGGGCAAGCTGATTGGCTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGc                     <  1:940228/100‑1 (MQ=255)
                                                                                 cTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCt                   >  2:147008/1‑100 (MQ=255)
                                                                                   gggCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTAt                 >  2:286552/1‑100 (MQ=255)
                                                                                     gCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCa               <  2:144765/100‑1 (MQ=255)
                                                                                      cAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCAc              >  2:604553/1‑100 (MQ=255)
                                                                                           tGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTGCCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGc          >  2:381677/1‑99 (MQ=255)
                                                                                             aTTGGTTTTTACGATCAAAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCAtg       >  2:1238223/1‑100 (MQ=255)
                                                                                               tGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCAtgtg     <  1:1036031/100‑1 (MQ=255)
                                                                                                ggTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGa    >  2:235811/1‑100 (MQ=255)
                                                                                                  tttttACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATc  <  2:549294/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATC  >  NC_000913/2580074‑2580271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: