Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,888,3830AG20.4% 106.8 / 24.4 56intergenic (‑71/+4)cysH/cysIphosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent/sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
Reads supporting (aligned to +/- strand):  ref base A (28/15);  new base G (8/3);  total (37/19)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

CAGGGCGTTTAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGCTTCGCGCTCTT  >  NC_000913/2888286‑2888481
                                                                                                 |                                                                                                  
cAGGGCGTTTAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATg                                                                                                  >  2:648716/1‑100 (MQ=255)
       tttAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATc                                                                                           >  2:157845/1‑100 (MQ=255)
       tttAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATc                                                                                           <  1:80533/100‑1 (MQ=255)
       tttAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTACTTATCAGGCCTACAAGACCGGGCTGATGGTTAATc                                                                                           <  1:920632/100‑1 (MQ=255)
         tAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGGTGGTTAATcc                                                                                          <  2:519601/99‑1 (MQ=255)
         tAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATccc                                                                                         <  2:1028515/100‑1 (MQ=255)
            aTCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCcaca                                                                                      <  1:466263/100‑1 (MQ=255)
             tCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGATCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACaa                                                                                     >  1:450476/1‑100 (MQ=255)
              cGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACaaa                                                                                    >  2:1284617/1‑100 (MQ=255)
               gAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAAt                                                                                   >  1:664370/1‑100 (MQ=255)
                aGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATc                                                                                  <  2:1145103/100‑1 (MQ=255)
                    tGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCAcgc                                                                              >  2:874256/1‑100 (MQ=255)
                     ggACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCAcgcg                                                                             >  1:684261/1‑100 (MQ=255)
                     ggACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCAcgcg                                                                             >  2:1024393/1‑100 (MQ=255)
                      gACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACgcgc                                                                            >  2:106169/1‑100 (MQ=255)
                         aTAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCgg                                                                         >  1:727355/1‑100 (MQ=255)
                          tAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGa                                                                        <  1:990596/100‑1 (MQ=255)
                          tAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGa                                                                        >  1:236938/1‑100 (MQ=255)
                           aGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGAt                                                                       >  1:357135/1‑100 (MQ=255)
                                 cTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCa                                                                 <  1:326089/100‑1 (MQ=255)
                                 cTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCa                                                                 >  1:532806/1‑100 (MQ=255)
                                   cACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCAcc                                                               >  2:921946/1‑100 (MQ=255)
                                       ntttGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGc                                                           >  2:398124/2‑100 (MQ=255)
                                       gTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGCTGGTTAATCCCCCCAATCAAGAGCCGGATAAAGCaaaggg                                                            >  1:433898/1‑94 (MQ=25)
                                       gTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGc                                                           >  2:621281/1‑100 (MQ=255)
                                         ttgccttgccTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGa                                                         >  1:1214520/1‑100 (MQ=255)
                                             cttgcctGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCACACAAATCACGCGCCGGATCGAGCACCGGGCGAatga                                                     >  2:878042/1‑100 (MQ=255)
                                              ttgcctGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAatgat                                                    <  2:499797/100‑1 (MQ=255)
                                               tgcctGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAatgatg                                                   >  1:1062880/1‑100 (MQ=255)
                                                        cGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCcgcacgc                                          >  1:517380/1‑100 (MQ=255)
                                                         gACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCcgcacgca                                         >  1:933150/1‑100 (MQ=255)
                                                           cGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCAcc                                       <  1:157847/100‑1 (MQ=255)
                                                           cGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCAcc                                       <  1:106171/100‑1 (MQ=255)
                                                            gCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCg                                      <  1:1048393/100‑1 (MQ=255)
                                                             cTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGt                                     <  1:938604/100‑1 (MQ=255)
                                                               cACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTaa                                   >  1:1130298/1‑100 (MQ=255)
                                                                   cgTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTc                               >  2:632579/1‑100 (MQ=255)
                                                                   cgTTCTTATCAGGCCTACAAGACCGGCCTGCCGGTTAATCCCACACACCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTc                               <  2:451883/100‑1 (MQ=255)
                                                                       cTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCg                           >  2:237055/1‑100 (MQ=255)
                                                                           tCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGc                       <  2:1038528/100‑1 (MQ=255)
                                                                              ggCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCtt                    >  1:628366/1‑100 (MQ=255)
                                                                              ggCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCtt                    >  2:170489/1‑100 (MQ=255)
                                                                              ggCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCtt                    >  1:578770/1‑100 (MQ=255)
                                                                               gCCTACAAGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTc                   >  1:488926/1‑100 (MQ=255)
                                                                               gCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTc                   <  2:1117830/100‑1 (MQ=255)
                                                                                ccTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCa                  >  2:524216/1‑100 (MQ=255)
                                                                                    cAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCg              >  2:437165/1‑100 (MQ=255)
                                                                                    cAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCAACCg              >  1:1008538/1‑100 (MQ=255)
                                                                                     aaGACCGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGc             >  2:338732/1‑100 (MQ=255)
                                                                                      aGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGCt            >  1:1236160/1‑100 (MQ=255)
                                                                                         ccGGGCTGGTGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGCTTcg         >  1:609062/1‑100 (MQ=255)
                                                                                         ccGGGCTGATGGTTAATCCAACAAATCACGCGCCGGCTCGAGCTCCGGGTAAATGATGATCGCACGATTCTTAAAGTCGTCGAATCCTTCACTCGCTTcg         <  2:433894/100‑1 (MQ=255)
                                                                                          cGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCAACCGATTcg         >  2:646962/1‑99 (MQ=255)
                                                                                            ggCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGCTTCgcgc      <  1:648720/100‑1 (MQ=255)
                                                                                             gCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGCTTCGCGct     <  1:80418/100‑1 (MQ=255)
                                                                                                gATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGCTTCGCGCTCtt  >  2:1142151/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
CAGGGCGTTTAGATCGAGTTTGGACATAGATTCCTCACTGTTTGCCTTGCCTGATGCGACGCTCACGCGTTCTTATCAGGCCTACAAGACCGGGCTGATGGTTAATCCCACAAATCACGCGCCGGATCGAGCACCGGGCGAATGATGCCCGCACGCACCGTAAAGTCGCCGAAGCCTTCACCCGCTTCGCGCTCTT  >  NC_000913/2888286‑2888481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: