Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	318,690	A→G	S438P (TCA→CCA)	rclA ←	reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	318,690	0	A	G	97.1%	236.3 / ‑2.4	70	S438P (TCA→CCA)	rclA	reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (34/34);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

AAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCA  >  NC_000913/318593‑318785                                                                                                  |                                                                                                aagaagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGgaa                                                                                               <  2:153981/100‑1 (MQ=255)  agaagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGgaaa                                                                                              >  1:334251/1‑100 (MQ=255)    aagTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTACTGGAAAt                                                                                             >  2:564565/1‑99 (MQ=255)     agTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAg                                                                                           <  2:193670/100‑1 (MQ=255)      gTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTTTGTTTTTTTTACTAATGGAAATAGa                                                                                          >  2:1157920/1‑100 (MQ=255)       ttATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGAt                                                                                         >  2:383743/1‑100 (MQ=255)       ttATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGAt                                                                                         >  2:385912/1‑100 (MQ=255)       ttATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGAt                                                                                         >  1:311404/1‑100 (MQ=255)        tataGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATc                                                                                        <  1:608197/100‑1 (MQ=255)         ataGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCa                                                                                       <  1:167805/100‑1 (MQ=255)          taGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCAt                                                                                      >  1:1280052/1‑100 (MQ=255)            gagTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTg                                                                                    <  1:51620/100‑1 (MQ=255)             agTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGa                                                                                   <  1:603724/100‑1 (MQ=255)              gTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAg                                                                                  <  2:391345/100‑1 (MQ=255)               tAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGt                                                                                 <  2:469207/100‑1 (MQ=255)               tAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGt                                                                                 <  1:941829/100‑1 (MQ=255)                 aCAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGa                                                                               >  1:53185/1‑100 (MQ=255)                  cAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGAt                                                                              >  2:371358/1‑100 (MQ=255)                   agagAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGAtt                                                                             <  1:1125796/100‑1 (MQ=255)                    gagaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTc                                                                            >  1:1247107/1‑100 (MQ=255)                    gagaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTc                                                                            <  1:93394/100‑1 (MQ=255)                    gagaATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTc                                                                            >  2:656941/1‑100 (MQ=255)                       aaTTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCt                                                                         <  1:965750/100‑1 (MQ=255)                             taaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGCCTAATGGAAATAGATCATTGAGTGATTCGCTCATCGa                                                                   <  2:699806/100‑1 (MQ=255)                              aaGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGAc                                                                  >  1:533785/1‑100 (MQ=255)                                gATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACgg                                                                >  2:834472/1‑100 (MQ=255)                                gATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACgg                                                                <  1:252726/100‑1 (MQ=255)                                gATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACgg                                                                <  2:1093348/100‑1 (MQ=255)                                 aTTCTTTTCTCTGAGACGCAAGCATCTTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATGGAGTGATTCGCTCATCGACg                                                                 >  2:107126/1‑98 (MQ=255)                                  ttCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGAt                                                              >  2:1280923/1‑100 (MQ=255)                                   tCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                             >  1:1051749/1‑100 (MQ=255)                                   tCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                             <  1:427875/100‑1 (MQ=255)                                   tCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATg                                                             >  1:1051826/1‑100 (MQ=255)                                      tttCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGt                                                          <  2:1247096/100‑1 (MQ=255)                                       ttCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTa                                                         >  2:338829/1‑100 (MQ=255)                                         ctctGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTaaa                                                       >  1:513769/1‑100 (MQ=255)                                          tctGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAat                                                      <  2:1223078/100‑1 (MQ=255)                                           atcagaCGCCAGAACAAATATTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAata                                                     >  1:1147013/4‑100 (MQ=37)                                             gagaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATc                                                   >  2:1262096/1‑100 (MQ=255)                                             gagaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATc                                                   <  1:979949/100‑1 (MQ=255)                                             gagaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATc                                                   <  1:154559/100‑1 (MQ=255)                                             cagaCGCAAGAACACATATTCTTGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGGGTGATTCGCTCATCGACGGATGAGTAAATATc                                                   >  2:216567/2‑100 (MQ=255)                                               gaCGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTg                                                 <  2:485/100‑1 (MQ=255)                                                 cGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGAt                                               >  1:933390/1‑100 (MQ=255)                                                   ccAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcg                                             >  1:589919/1‑100 (MQ=255)                                                    cAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATcgc                                            >  2:43331/1‑100 (MQ=255)                                                        atatTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTaa                                        <  1:671180/100‑1 (MQ=255)                                                          atTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAat                                       >  1:572844/1‑99 (MQ=255)                                                             tGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGc                                   <  1:429177/100‑1 (MQ=255)                                                             tGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGc                                   <  1:308044/100‑1 (MQ=255)                                                              gTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCt                                  >  2:373155/1‑100 (MQ=255)                                                                   ggCGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAg                             >  2:1022696/1‑100 (MQ=255)                                                                     cGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCTTCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGc                           <  2:1190530/100‑1 (MQ=255)                                                                     cGTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGc                           <  2:787899/100‑1 (MQ=255)                                                                      gTCTGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCa                          <  2:828694/100‑1 (MQ=255)                                                                         tGATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGcc                       <  1:1183381/100‑1 (MQ=255)                                                                          gATTTTGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGccc                      >  1:451998/1‑100 (MQ=255)                                                                            ttttGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCgg                    <  2:1203077/100‑1 (MQ=255)                                                                              ttGAGTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGca                  >  1:263374/1‑100 (MQ=255)                                                                                  gTTTATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGcatcca              >  1:991289/1‑100 (MQ=255)                                                                                     tATTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCa           >  1:562556/1‑100 (MQ=255)                                                                                      aTTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCa           <  2:813610/99‑1 (MQ=255)                                                                                      aTTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc          <  2:390086/100‑1 (MQ=255)                                                                                      aTTTGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAc          <  1:1224894/100‑1 (MQ=255)                                                                                       tttGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAcc         >  2:1055495/1‑100 (MQ=255)                                                                                       tttGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAcc         >  2:400299/1‑100 (MQ=255)                                                                                       tttGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAcc         <  2:912248/100‑1 (MQ=255)                                                                                       nttGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCAcc         >  1:598084/2‑100 (MQ=255)                                                                                        ttGACTAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCa        <  1:338832/100‑1 (MQ=255)                                                                                             tAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTc   >  2:367583/1‑100 (MQ=255)                                                                                             tAATGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTc   >  1:103946/1‑100 (MQ=255)                                                                                              aaTGGAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCa  <  1:643669/100‑1 (MQ=255)                                                                                                  |                                                                                                AAGAAGTTATAGAGTAACAGAGAATTAATAAGATTCTTTTCTCTGAGACGCCAGAATATTTGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCA  >  NC_000913/318593‑318785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑