| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,308,182 | A→T | L145Q (CTG→CAG) | yojI ← | microcin J25 efflux ABC transporter permease/ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,308,182 | 0 | A | T | 97.8% | 156.8 / ‑3.6 | 46 | L145Q (CTG→CAG) | yojI | microcin J25 efflux ABC transporter permease/ATPase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (27/18); total (28/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GTCGCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACAT > NC_000913/2308086‑2308275 | gTCGCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCa > 2:812710/1‑100 (MQ=255) gCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGGCCGACTGCAt > 2:1063438/1‑98 (MQ=255) gCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATcc > 1:755013/1‑100 (MQ=255) gCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATcc < 2:1236774/100‑1 (MQ=255) ccATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCca > 1:964824/1‑100 (MQ=255) tGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGAt > 1:257504/1‑100 (MQ=255) gTTTGTACACGCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCAGCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGAt > 2:1275382/1‑99 (MQ=255) ttGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATAcg < 2:305381/100‑1 (MQ=255) acacCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATAcgccgc < 1:29275/100‑1 (MQ=255) acacCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATAcgccgc < 1:977974/100‑1 (MQ=255) cacCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCt > 1:474699/1‑100 (MQ=255) cccGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTg > 2:1015667/1‑99 (MQ=255) cgcgCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAAc > 1:1006116/1‑100 (MQ=255) gcgcCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAAcc < 1:617427/100‑1 (MQ=255) ccaGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGt < 2:780304/100‑1 (MQ=255) cacaAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTgaga < 1:533221/100‑1 (MQ=255) caAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAAt > 1:1009316/1‑100 (MQ=255) caAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAAt < 1:360146/100‑1 (MQ=255) aaCCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGAt > 1:597976/1‑100 (MQ=255) aaCCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGAt < 2:755008/100‑1 (MQ=255) ccGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATcc > 2:158275/1‑100 (MQ=255) gCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATcccc < 2:1285223/100‑1 (MQ=255) ccccAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCt < 1:1287913/100‑1 (MQ=255) cAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCa < 1:369140/100‑1 (MQ=255) gATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTcc < 1:439759/100‑1 (MQ=255) gATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTcc > 2:14789/1‑100 (MQ=255) gATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTcc > 2:64235/1‑100 (MQ=255) gATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTcc > 1:212740/1‑100 (MQ=255) aTCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCg < 1:1021859/100‑1 (MQ=255) cGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGc < 2:395178/100‑1 (MQ=255) gCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCa < 1:30984/100‑1 (MQ=255) gCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCa < 2:96709/100‑1 (MQ=255) cATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGTACCGAGAGTGAGAATCATCCCCCGCACCACTTCCGGc > 2:736172/1‑97 (MQ=255) cATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCa > 2:1095455/1‑98 (MQ=255) aTCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGAc > 1:512563/1‑100 (MQ=255) aTCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGAc > 2:169023/1‑100 (MQ=255) tCCAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACg > 1:796386/1‑100 (MQ=255) cAGATAGCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGca > 2:326396/1‑100 (MQ=255) gCCGTTACCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAg > 2:1295955/1‑100 (MQ=255) aCCAGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATgg < 2:10402/100‑1 (MQ=255) aGCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGa > 1:1127995/1‑100 (MQ=255) gCAACATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGat > 2:1097558/1‑100 (MQ=255) aCATTTTGCCCGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGATAtt > 2:819508/1‑100 (MQ=255) cccGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACCAAAGCAATGGTGATATTGCGCACAt > 1:1198114/1‑100 (MQ=255) cccGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACAt > 2:666930/1‑100 (MQ=255) cccGACTGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACAt > 1:322946/1‑100 (MQ=255) | GTCGCCATATGTTTGTACACCCGCGCCACCAGCACAAAACCGCCCCAGATGGTGATCGCCATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACAT > NC_000913/2308086‑2308275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |