| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,457,953 | T→C | K403E (AAA→GAA) | fadJ ← | enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,457,953 | 0 | T | C | 92.1% | 110.0 / 1.0 | 38 | K403E (AAA→GAA) | fadJ | enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (20/15); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
TGAGCGGCGATATCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTTCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCTGTTTGTC > NC_000913/2457856‑2458049 | tgAGCGGCGATATCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTTCa < 2:516083/100‑1 (MQ=255) gAGCGGCGATATCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCaa > 2:92253/1‑100 (MQ=255) gAGCGGCGATATCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCaa < 2:55930/100‑1 (MQ=255) gcggcgATATCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAAtt < 1:441171/100‑1 (MQ=255) atCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGAttt < 1:630937/100‑1 (MQ=255) atCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGAttt < 2:103202/100‑1 (MQ=255) aCCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGTTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCa < 1:520072/100‑1 (MQ=255) ccAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCaa < 1:994587/100‑1 (MQ=255) ggTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCg > 1:1152434/1‑100 (MQ=255) aGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCa < 2:1112797/100‑1 (MQ=255) gACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCaataa > 2:1145820/1‑100 (MQ=255) aCGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCaataat < 2:1040898/100‑1 (MQ=255) cGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATc > 1:456225/1‑100 (MQ=255) cGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATc < 2:5443/100‑1 (MQ=255) aTTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGa > 1:1270793/1‑100 (MQ=255) aTTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGa > 2:596640/1‑100 (MQ=255) cGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATcg > 2:884781/1‑100 (MQ=255) cGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATcg > 2:53291/1‑100 (MQ=255) gAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTTCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATcgc > 1:352680/1‑100 (MQ=255) aaGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATcgcg > 2:35738/1‑100 (MQ=255) aGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGa < 1:1090113/100‑1 (MQ=255) cAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATg < 2:890237/100‑1 (MQ=255) aaGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATggg < 2:1140772/100‑1 (MQ=255) aTGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCcg > 2:616940/1‑100 (MQ=255) gAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCcgcg > 2:1247447/1‑100 (MQ=255) gAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCcgcg > 2:739687/1‑100 (MQ=255) ggcgCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAg > 1:1218159/1‑100 (MQ=255) aTTTTGCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGCCAAAGCCGCGATAGTCcgtc > 1:1012540/1‑100 (MQ=255) gCTCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTcc > 2:138087/1‑100 (MQ=255) tCAACTTCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGTTCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCgg < 2:700284/100‑1 (MQ=255) ttCCGCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGAtt > 1:1196670/1‑100 (MQ=255) gCCACCATCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTaacg < 1:885614/100‑3 (MQ=255) aTCTGTTGTTCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCt > 1:815830/1‑100 (MQ=255) tCTGTTGTTTCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCTg < 2:352677/100‑1 (MQ=255) ttgttCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCTGTTTg > 2:1164405/1‑100 (MQ=255) gttCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCTGTTTg < 2:117764/98‑1 (MQ=255) gttCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCTGTTTGTc > 2:511750/1‑100 (MQ=255) gttCCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCTGTTTGTc > 2:192621/1‑100 (MQ=255) | TGAGCGGCGATATCACCAATCGGTAAAGATGACGTATTCGAAGCAAAGATGGTATGAGCGGCGCAATTTTGCTCAACTTCCGCCACCATCTGTTGTTTCAATTCGAGATTTTCAAACACCGCTTCAATAATCAGATCGCGATGGGCAAAGCCGCGATAGTCCGTCGTTCCGGAGATTAATGCCAGCTGTTTGTC > NC_000913/2457856‑2458049 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |