Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,461,483 G→T A60S (GCA→TCA)  fadL → long‑chain fatty acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,461,4830GT95.2% 207.5 / 0.8 62A60S (GCA→TCA) fadLlong‑chain fatty acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (23/36);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TTTCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTC  >  NC_000913/2461387‑2461576
                                                                                                |                                                                                             
tttCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCAt                                                                                            >  1:617046/1‑100 (MQ=255)
tttCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCAt                                                                                            >  1:344419/1‑100 (MQ=255)
  tCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTg                                                                                          >  1:920018/1‑100 (MQ=255)
     gTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGAtt                                                                                       >  1:694869/1‑100 (MQ=255)
     gTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGAtt                                                                                       <  2:838951/100‑1 (MQ=255)
      ttAAACGAATTTTCTTTCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTa                                                                                      <  2:761611/100‑1 (MQ=255)
      ttAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTa                                                                                      <  2:214318/100‑1 (MQ=255)
        aaaCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTAc                                                                                     <  2:128741/99‑1 (MQ=255)
          aCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTAt                                                                                  <  2:514396/100‑1 (MQ=255)
           cGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATg                                                                                 >  1:1151555/1‑100 (MQ=255)
             aaTTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGtt                                                                               <  1:148962/100‑1 (MQ=255)
              aTTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGACGCAATTGCCGATGATGCAAGTAACGGCAGCCGTAACCCCGCATTGATTACTATGtt                                                                               >  2:713003/1‑99 (MQ=255)
               ttttCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTg                                                                             <  2:730820/100‑1 (MQ=255)
               ttttCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTg                                                                             <  1:1117457/100‑1 (MQ=255)
                 ttcttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGAc                                                                           <  1:577979/100‑1 (MQ=255)
                  tcttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATCTTTGAcc                                                                          <  1:684105/100‑1 (MQ=255)
                   cttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCg                                                                         >  1:739177/1‑100 (MQ=255)
                        tctGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTTACCGCCCGa                                                                    >  2:326476/1‑100 (MQ=255)
                         ctGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGAc                                                                   >  2:203937/1‑100 (MQ=255)
                         ctGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGAc                                                                   <  2:532979/100‑1 (MQ=255)
                            gCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACAtt                                                                >  2:1068614/1‑100 (MQ=255)
                             ccTGGGCCGGGCTTTATAAGGAGCAGGCGCAATTACCGATGATGCAGGTAATGTGAGGCTGAAGCTCTCATTGGTTACTATTGTTGACCGCCCTACAtgt                                                               >  2:433611/1‑98 (MQ=255)
                                gggCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCt                                                            <  1:583213/100‑1 (MQ=255)
                                 ggCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTg                                                           >  1:151730/1‑100 (MQ=255)
                                  gCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGc                                                          >  2:59245/1‑100 (MQ=255)
                                          tATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCgg                                                  >  1:178741/1‑100 (MQ=255)
                                           aTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTATCGTCAGCCGTAACCCCTCATTGATTACTATGTTTGTCCGCCCGACATTTTCTGCGGGTGCGGt                                                 <  1:915526/100‑1 (MQ=255)
                                           aTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAGCGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGt                                                 <  1:915524/100‑1 (MQ=255)
                                           aTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGt                                                 >  1:132867/1‑100 (MQ=255)
                                            ttCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGtt                                                <  2:617042/100‑1 (MQ=255)
                                            ttCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGtt                                                <  2:698219/100‑1 (MQ=255)
                                            ttCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGtt                                                >  2:941108/1‑100 (MQ=255)
                                              cAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTta                                              <  1:975957/100‑1 (MQ=255)
                                              cAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTta                                              <  1:379561/100‑1 (MQ=255)
                                                 gggAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAAACCCTCATTGATTACTATGTTTGAGCGCCCGACATTTTCTGCGGGTGCGGTTTatat                                           >  2:767949/1‑100 (MQ=255)
                                                      ggCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAcc                                      >  1:1155035/1‑100 (MQ=255)
                                                       gcgcAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAccc                                     <  1:554271/100‑1 (MQ=255)
                                                        cgcAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCg                                    <  2:1155024/100‑1 (MQ=255)
                                                         gcAATTGCCGATGATGCAGGTACCGTCAGCCTTAACCACTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCgg                                   <  2:877183/100‑1 (MQ=255)
                                                         gcAATTGCCGATGATGCAGGTAACGTCCGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCgg                                   <  2:231241/100‑1 (MQ=255)
                                                          cAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGa                                  >  1:440493/1‑100 (MQ=255)
                                                            aTTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATg                                <  2:920010/100‑1 (MQ=255)
                                                               gCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTa                              >  1:672264/1‑99 (MQ=255)
                                                                 cGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAat                           <  1:1198631/100‑1 (MQ=255)
                                                                  gatgatGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAata                          <  1:260147/100‑1 (MQ=255)
                                                                   atgatgCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAatat                         <  2:178739/100‑1 (MQ=255)
                                                                    tgatgCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATc                        <  2:1040653/100‑1 (MQ=255)
                                                                       tgCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGc                     <  2:263420/100‑1 (MQ=255)
                                                                          aGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGa                  <  1:924129/100‑1 (MQ=255)
                                                                            gTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAAc                <  1:368595/100‑1 (MQ=255)
                                                                             tAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACg               <  1:1055569/100‑1 (MQ=255)
                                                                                 gTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtctc           <  2:445807/100‑1 (MQ=255)
                                                                                 gTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtctc           >  1:852188/1‑100 (MQ=255)
                                                                                 gTCACCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGACCGtctc           >  2:1016534/1‑100 (MQ=255)
                                                                                  tCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTcc          >  2:818205/1‑100 (MQ=255)
                                                                                  tCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTcc          <  1:1116495/100‑1 (MQ=255)
                                                                                    aGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCAt        >  2:432052/1‑100 (MQ=255)
                                                                                     gCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTTCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATc       <  1:499554/100‑1 (MQ=255)
                                                                                     gCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATc       >  2:159415/1‑100 (MQ=255)
                                                                                        gTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTgg    <  1:60829/100‑1 (MQ=255)
                                                                                          aaCCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTc  <  1:1246847/100‑1 (MQ=255)
                                                                                          aaCCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTc  <  2:621444/100‑1 (MQ=255)
                                                                                                |                                                                                             
TTTCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTC  >  NC_000913/2461387‑2461576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: