Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,715,439 T→C L6S (TTA→TCA)  eamB → cysteine and O‑acetylserine exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,715,4390TC100.0% 211.6 / NA 60L6S (TTA→TCA) eamBcysteine and O‑acetylserine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/32);  total (28/32)

CGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCA  >  NC_000913/2715344‑2715535
                                                                                               |                                                                                                
cGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGt                                                                                              >  2:1118628/1‑100 (MQ=255)
cGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAAATAACAAGTGACACCGACACTTTCAAGt                                                                                              >  2:123479/1‑100 (MQ=255)
 gAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTg                                                                                             <  1:1177839/100‑1 (MQ=255)
   aTCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCt                                                                                           <  2:140058/100‑1 (MQ=255)
      tAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCtttt                                                                                        >  2:1273298/1‑100 (MQ=255)
         aaTATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTgg                                                                                     <  2:281698/100‑1 (MQ=255)
         aaTATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTgg                                                                                     <  2:464992/100‑1 (MQ=255)
         aaTATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTgg                                                                                     <  1:201146/100‑1 (MQ=255)
           tataAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGAc                                                                                   <  2:478121/100‑1 (MQ=255)
            ataAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACt                                                                                  <  2:1003380/100‑1 (MQ=255)
             taAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACtt                                                                                 >  2:679204/1‑100 (MQ=255)
               aaaaCATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTac                                                                               >  1:1265324/1‑100 (MQ=255)
                aaaCATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTaca                                                                              <  1:788568/100‑1 (MQ=255)
                  aCATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACAcc                                                                            <  1:572437/100‑1 (MQ=255)
                    attcattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCt                                                                          >  1:1038135/1‑100 (MQ=255)
                       cattTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGAt                                                                       >  1:639998/1‑100 (MQ=255)
                           tttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTAcc                                                                   <  2:542472/100‑1 (MQ=255)
                           tttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTAcc                                                                   <  2:120479/100‑1 (MQ=255)
                            ttttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCg                                                                  <  2:669460/100‑1 (MQ=255)
                             tttAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGc                                                                 >  2:77364/1‑100 (MQ=255)
                               tAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTa                                                               <  2:714492/100‑1 (MQ=255)
                                   tGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGAc                                                           >  1:35827/1‑100 (MQ=255)
                                   tGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGAc                                                           <  1:1140850/100‑1 (MQ=255)
                                   tGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGAc                                                           <  2:825248/100‑1 (MQ=255)
                                      tCCGTGTGGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGcc                                                        >  1:686061/1‑100 (MQ=255)
                                      tCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGcc                                                        <  1:965642/100‑1 (MQ=255)
                                      tCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGcc                                                        >  1:316988/1‑100 (MQ=255)
                                      tCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGcc                                                        >  1:1091767/1‑100 (MQ=255)
                                        cGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAg                                                      <  2:1183199/100‑1 (MQ=255)
                                         ntgtCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAgg                                                     >  1:59116/2‑100 (MQ=255)
                                            tCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGAcc                                                  <  1:1265991/100‑1 (MQ=255)
                                             cGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCg                                                 <  1:758231/100‑1 (MQ=255)
                                              gggTACTGTCTACCAAAACAGAGGAGATAACAAGTGAGACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGa                                                >  2:697596/1‑100 (MQ=255)
                                                gTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAAc                                              <  1:1011956/100‑1 (MQ=255)
                                                 tACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAgatcggaaga                                             >  2:910802/1‑91 (MQ=255)
                                                  aCTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTTTTTTTTTTTCCTGATTACCGCTATGACGCCAGGACCGaa                                               >  2:382882/1‑97 (MQ=255)
                                                     gTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAatat                                         >  2:982883/1‑100 (MQ=255)
                                                          ccAAAACAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCg                                    <  1:901036/100‑1 (MQ=255)
                                                            aaaaCAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGcc                                  <  2:1139914/100‑1 (MQ=255)
                                                              aaCAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCt                                <  1:679208/100‑1 (MQ=255)
                                                               aCAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCtt                               <  2:556666/100‑1 (MQ=255)
                                                                cAGAGGAGATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTa                              >  2:319281/1‑100 (MQ=255)
                                                                  gaggagATAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGc                            >  1:708945/1‑100 (MQ=255)
                                                                      agaTAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTg                        <  1:528448/100‑1 (MQ=255)
                                                                      agaTAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTg                        <  1:836212/100‑1 (MQ=255)
                                                                       gaTAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGc                       >  1:738100/1‑100 (MQ=255)
                                                                         tAACAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTa                     >  2:132131/1‑100 (MQ=255)
                                                                          aaCAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTAc                    <  1:1034912/100‑1 (MQ=255)
                                                                            cAAGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGt                  >  1:751292/1‑100 (MQ=255)
                                                                              aGTGACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCg                <  1:59358/100‑1 (MQ=255)
                                                                                 gACACCGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCAt             >  2:565993/1‑100 (MQ=255)
                                                                                     ccGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAt         >  1:394375/1‑100 (MQ=255)
                                                                                      cGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAtt        >  1:1037957/1‑100 (MQ=255)
                                                                                      cGACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAtt        <  1:1006445/100‑1 (MQ=255)
                                                                                       gACCCTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAttt       >  2:293227/1‑100 (MQ=255)
                                                                                       gACCCTTTCAAGTGCTTTTTGAACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAttt       <  1:123481/100‑1 (MQ=255)
                                                                                           cTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTc   >  1:427652/1‑100 (MQ=255)
                                                                                           cTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTc   <  1:657851/100‑1 (MQ=255)
                                                                                           cTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTc   <  2:253603/100‑1 (MQ=255)
                                                                                           cTTTCAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTc   >  2:175306/1‑100 (MQ=255)
                                                                                            tttcAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCa  <  2:886043/100‑1 (MQ=255)
                                                                                            tttcAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAGTTCGTCa  >  1:1304216/1‑100 (MQ=255)
                                                                                               |                                                                                                
CGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCA  >  NC_000913/2715344‑2715535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: