Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,720,543	C→A	R197R (CGC→CGA)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,720,543	0	C	A	100.0%	189.3 / NA	55	R197R (CGC→CGA)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/29);  total (26/29)

GATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGCGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACC  >  NC_000913/2720465‑2720638                                                                               |                                                                                                gATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATc                                                                            >  2:735195/1‑100 (MQ=255)  aTGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGACGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATcc                                                                           <  2:722877/100‑1 (MQ=255)   tGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCt                                                                          >  2:1070223/1‑100 (MQ=255)     ggCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGc                                                                        <  2:663667/100‑1 (MQ=255)      gCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGct                                                                       <  2:727298/100‑1 (MQ=255)        ttttCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGctct                                                                     <  1:851129/100‑1 (MQ=255)            ccTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTAtct                                                                 >  2:421502/1‑100 (MQ=255)               aCTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGa                                                              <  2:1100374/100‑1 (MQ=255)               aCTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGa                                                              <  1:1161512/100‑1 (MQ=255)               aCTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGa                                                              >  1:480182/1‑100 (MQ=255)                cTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGaa                                                             >  1:275460/1‑100 (MQ=255)                 tttATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAAc                                                            <  1:1149324/100‑1 (MQ=255)                 tttATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAAc                                                            <  1:345043/100‑1 (MQ=255)                  ttATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACa                                                           >  2:184992/1‑100 (MQ=255)                    aTTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGt                                                         >  1:1065524/1‑100 (MQ=255)                    aTTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGt                                                         <  1:134461/100‑1 (MQ=255)                     ttGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGtt                                                        >  1:1026332/1‑100 (MQ=255)                       gcgcTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTaa                                                      >  2:173602/1‑100 (MQ=255)                        cgcTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAg                                                     >  2:258306/1‑100 (MQ=255)                         gcTCGGCAACAGCCTGGATATCGAAGTTGATTAATTGCTTGACTAACGGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgc                                                    <  2:1018604/100‑1 (MQ=255)                             ggCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcgacg                                                >  2:939583/1‑100 (MQ=255)                             ggCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcga                                                  <  1:358506/98‑1 (MQ=255)                              gCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcgacgc                                               <  1:1230191/100‑1 (MQ=255)                               cGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcgacgcg                                              >  2:1264312/1‑100 (MQ=255)                                gACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcgacgcgc                                             >  2:835597/1‑100 (MQ=255)                                    gCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcgacgcgcgacg                                         >  1:638834/1‑100 (MQ=255)                                    gCCTGGAGATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcgacgcgcgacg                                         >  1:686937/1‑100 (MQ=255)                                     ccTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAgcgacgcgcgacgc                                        >  1:450179/1‑100 (MQ=255)                                        ggATATCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCttt                                     <  1:946023/100‑1 (MQ=255)                                            atCGACGTTGATGGATTGCTCGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGttt                                 <  2:503402/100‑1 (MQ=255)                                             tCGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTc                                <  1:790850/100‑1 (MQ=255)                                              cGACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTc                                <  2:1143799/99‑1 (MQ=255)                                               gACGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTcgg                              >  2:1169344/1‑100 (MQ=255)                                               gACGTTGATGAATTGCTGGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTcgg                              <  1:905645/100‑1 (MQ=255)                                                aCGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTcggc                             <  1:258309/100‑1 (MQ=255)                                                 cGTTGATGAATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTcggcg                            >  1:1004236/1‑100 (MQ=255)                                                   ttGATGAATTGCTTGACTATCTGGCACGAGAAAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCggcggc                          >  1:510619/1‑100 (MQ=255)                                                    tgatgaATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGcc                         <  2:1133434/100‑1 (MQ=255)                                                     gatgaATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGccc                        >  2:651838/1‑100 (MQ=255)                                                       tgaATTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGt                      <  2:982197/100‑1 (MQ=255)                                                         aaTTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAg                    <  2:275457/100‑1 (MQ=255)                                                          aTTGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGt                   <  1:1070233/100‑1 (MQ=255)                                                           ttGCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTg                  <  1:216283/100‑1 (MQ=255)                                                             gCTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGcc                <  1:789537/100‑1 (MQ=255)                                                              cTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCt               >  1:980713/1‑100 (MQ=255)                                                              cTTGACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGACCGTAGTGCCt               >  2:1002595/1‑100 (MQ=255)                                                                 gACTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTcgc            >  1:1147682/1‑100 (MQ=255)                                                                   cTATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGt          >  1:816658/1‑100 (MQ=255)                                                                    tATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGta         <  2:45422/100‑1 (MQ=255)                                                                    tATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGta         <  2:501487/100‑1 (MQ=255)                                                                    tATCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGta         <  1:1264323/100‑1 (MQ=255)                                                                      tCTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGtaat       <  1:668898/100‑1 (MQ=255)                                                                       cTGGCACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGtaata      >  1:184438/1‑100 (MQ=255)                                                                          gCACGAGACACTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAAc   <  2:855102/100‑1 (MQ=255)                                                                           cACGAGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAAcc  >  2:1266991/1‑100 (MQ=255)                                                                               |                                                                                                GATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTGACTATCTGGCACGCGACAGTAAAACCAGCGCCATCCTGCTCTATCTCGAACAGTTAAGCGACGCGCGACGCTTTGTTTCGGCGGCCCGTAGTGCCTCGCGTAATAAACC  >  NC_000913/2720465‑2720638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑