| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,759,497 | T→C | C171C (TGT→TGC) | yfjI → | CP4‑57 prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,759,497 | 0 | T | C | 98.3% | 198.4 / ‑4.2 | 59 | C171C (TGT→TGC) | yfjI | CP4‑57 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (28/30); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
GACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGA > NC_000913/2759400‑2759588 | gACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCgg < 1:102692/100‑1 (MQ=255) aCTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCggg < 2:17215/100‑1 (MQ=255) aCTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCggg < 1:649398/100‑1 (MQ=255) cTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGc > 2:474002/1‑100 (MQ=255) tAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCa < 1:474006/99‑1 (MQ=255) tAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCAc > 2:595167/1‑100 (MQ=255) tAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCAc > 2:658080/1‑100 (MQ=255) aaGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTg > 1:950448/1‑100 (MQ=255) gAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGc < 2:115688/100‑1 (MQ=255) gAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGc < 2:115850/100‑1 (MQ=255) aaCTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCg > 1:332545/1‑100 (MQ=255) acttctTATGACAAACCAGAAAGCTCCAGTGATGTTCAAATTTTTATTTAATGATTTCACTCCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGa > 2:674785/1‑100 (MQ=255) actactTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGa < 2:8832/100‑1 (MQ=255) ctactTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGAt < 1:1271861/100‑1 (MQ=255) tactTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATc < 1:1198847/100‑1 (MQ=255) tactTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATc < 1:696913/100‑1 (MQ=255) actTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCa > 1:28968/1‑100 (MQ=255) ctTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAg > 2:763067/1‑100 (MQ=255) ctTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAg > 1:423727/1‑100 (MQ=255) aCAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGca > 2:675400/1‑100 (MQ=255) cAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGcat > 2:1282931/1‑100 (MQ=255) aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGcatc < 2:192406/100‑1 (MQ=255) aaaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGcatc < 2:850152/100‑1 (MQ=255) aaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGcatca < 1:1046369/100‑1 (MQ=255) aaCCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGcatca < 1:1122357/100‑1 (MQ=255) cGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTc > 2:783995/1‑100 (MQ=255) aGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCtgat < 1:287734/100‑1 (MQ=255) cTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCtgatga > 2:22467/1‑100 (MQ=255) tCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGaa > 2:195470/1‑100 (MQ=255) ccAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAg < 1:142395/100‑1 (MQ=255) cAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGc < 2:312546/100‑1 (MQ=255) aGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCt > 1:276604/1‑100 (MQ=255) gTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTACAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAGGCTg > 2:1053175/1‑100 (MQ=255) tGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTgg < 1:1300104/100‑1 (MQ=255) gagaTTCAAATTTTTATTTAACGATGCCACGCCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTggg < 2:177425/100‑1 (MQ=255) gagaTTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTggg > 2:1002069/1‑100 (MQ=255) agaTTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGa < 1:518911/100‑1 (MQ=255) ttCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCa > 1:1195902/1‑100 (MQ=255) cAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCAtt > 1:366294/1‑100 (MQ=255) aaTTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCAtttt > 1:200729/1‑100 (MQ=255) aTTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCAttttt < 1:824684/100‑1 (MQ=255) tttatttaACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAAt < 2:282907/100‑1 (MQ=255) ttatttaACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATg < 2:895036/100‑1 (MQ=255) tatttaACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATgg > 1:999362/1‑100 (MQ=255) tttaACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGtt < 1:81772/100‑1 (MQ=255) ttaACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTa < 1:652521/100‑1 (MQ=255) ttaACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTa < 1:1002078/100‑1 (MQ=255) taACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTac < 2:366291/100‑1 (MQ=255) aCGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAATCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTacac < 1:758710/100‑1 (MQ=255) cGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTgcaca > 2:525934/1‑100 (MQ=255) ccACACCTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTaa > 1:862390/1‑100 (MQ=255) ccTGCAGCTATTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGc > 1:1174889/1‑100 (MQ=255) cTGCAGCTATTATAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCt < 1:551643/100‑1 (MQ=255) tGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTg > 1:717912/1‑100 (MQ=255) aTTAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTa > 1:957697/1‑100 (MQ=255) ttAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTAt > 2:510824/1‑100 (MQ=255) tAAAGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATc < 1:212356/100‑1 (MQ=255) aGCTCATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAAt > 1:1288968/1‑100 (MQ=255) cATCTCTGCGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGa > 2:610772/1‑100 (MQ=255) | GACTTAAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGATGCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATGTCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAATAAGA > NC_000913/2759400‑2759588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |