Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,852,356	T→A	L74Q (CTG→CAG)	hypD →	hydrogenase maturation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,852,356	0	T	A	100.0%	184.7 / NA	53	L74Q (CTG→CAG)	hypD	hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/28);  total (25/28)

GCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAA  >  NC_000913/2852260‑2852450                                                                                                 |                                                                                               gCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGcc                                                                                             <  2:293123/100‑1 (MQ=255) gCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGcc                                                                                             <  1:334777/100‑1 (MQ=255)     tCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATg                                                                                         >  2:1130758/1‑100 (MQ=255)        tACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                       >  2:1035249/1‑99 (MQ=255)        tACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGt                                                                                      <  2:1179652/100‑1 (MQ=255)          cccACGCTATCTTTAAATTCGGCCTCGACCAGTTACGGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAg                                                                                    <  2:138625/100‑1 (MQ=255)               gCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCg                                                                               >  1:948163/1‑100 (MQ=255)                   tCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGacac                                                                           >  1:258430/1‑100 (MQ=255)                     tttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCt                                                                         >  2:544100/1‑100 (MQ=255)                     tttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCt                                                                         >  1:988355/1‑100 (MQ=255)                        aaaTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCg                                                                      <  1:350876/100‑1 (MQ=255)                           ttCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTgg                                                                   <  2:39041/100‑1 (MQ=255)                            tCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGga                                                                  <  1:427641/100‑1 (MQ=255)                            tCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGga                                                                  <  1:427619/100‑1 (MQ=255)                             cgtccTCGACCAGTAATTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGgag                                                                 <  2:888664/97‑1 (MQ=255)                              ggCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGgaga                                                                <  1:1168976/100‑1 (MQ=255)                               gCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAt                                                               >  1:807816/1‑100 (MQ=255)                                      ccAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagc                                                        <  1:542026/100‑1 (MQ=255)                                       cAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagcc                                                       <  2:988347/100‑1 (MQ=255)                                         gTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTACGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCAt                                                     >  1:748410/1‑100 (MQ=255)                                            aCTGCCGGAAAACGTTGAGGTTATCCACGCTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATcc                                                   >  2:83161/1‑99 (MQ=255)                                             cTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACCCCTGCGTGGAGATTGCCAGCCATCCgg                                                 >  2:1080157/1‑100 (MQ=255)                                             cTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGCAGAATGGACACCTGAGTGGAGATTGCCAGCCATCCgg                                                 >  2:881037/1‑100 (MQ=255)                                              tGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGa                                                <  2:166830/100‑1 (MQ=255)                                               gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGaa                                               >  1:238717/1‑100 (MQ=255)                                                 cGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGt                                             <  1:654839/100‑1 (MQ=255)                                                      aaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtct                                        <  1:1108499/100‑1 (MQ=255)                                                        cGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAACTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtct                                        <  2:152976/98‑1 (MQ=255)                                                          ttGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTg                                    >  1:1024751/1‑100 (MQ=255)                                                           tGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGt                                   <  1:630319/100‑1 (MQ=255)                                                              gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAcc                                >  1:911501/1‑100 (MQ=255)                                                              gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAcc                                >  2:1208148/1‑100 (MQ=255)                                                                 tATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCttt                             >  1:92339/1‑100 (MQ=255)                                                                 tATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCttt                             >  2:598126/1‑100 (MQ=255)                                                                    ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCtt                              >  1:148509/1‑96 (MQ=255)                                                                    ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                          >  2:153729/1‑100 (MQ=255)                                                                    ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                          <  1:478904/100‑1 (MQ=255)                                                                    ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                          >  2:1222469/1‑100 (MQ=255)                                                                    ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                          >  2:1181919/1‑100 (MQ=255)                                                                        ggTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAGCGGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACg                      <  2:407064/100‑1 (MQ=255)                                                                        ggTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACg                      <  1:1200618/100‑1 (MQ=255)                                                                         gTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGc                     <  1:1130768/100‑1 (MQ=255)                                                                           ccGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCa                   <  2:1221852/100‑1 (MQ=255)                                                                            cGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCAt                  >  2:1197435/1‑100 (MQ=255)                                                                               ggTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATgcg               >  1:944444/1‑100 (MQ=255)                                                                                   cccGGTGTGAGTACAGCCGATGGGTAGAATCGCAACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCCTCTTCTGTACCTTTGGCGACGCCATGCGCGTg           <  1:385814/100‑1 (MQ=255)                                                                                    ccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGc          <  2:135873/100‑1 (MQ=255)                                                                                    ccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGc          >  2:442019/1‑100 (MQ=255)                                                                                    ccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGc          >  1:544282/1‑100 (MQ=255)                                                                                         gtgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCgggg     <  1:1153462/100‑1 (MQ=255)                                                                                         gtgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCgggg     <  1:161063/100‑1 (MQ=255)                                                                                         gtgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCgggg     <  1:29474/100‑1 (MQ=255)                                                                                            cGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaaa  <  2:1070820/100‑1 (MQ=255)                                                                                                 |                                                                                               GCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAA  >  NC_000913/2852260‑2852450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑