Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,887,352 T→A intergenic (‑133/+226) ygcB ← / ← cysH Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,887,3520TA98.2% 199.2 / ‑4.0 57intergenic (‑133/+226)ygcB/cysHCascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (27/29);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

TCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC  >  NC_000913/2887257‑2887447
                                                                                               |                                                                                               
tCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGttt                                                                                             >  1:1122131/1‑100 (MQ=255)
 cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg                                                                                            >  1:576036/1‑100 (MQ=255)
 cAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTg                                                                                            >  2:737954/1‑100 (MQ=255)
  aGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTgg                                                                                           <  1:271527/100‑1 (MQ=255)
   gCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTggg                                                                                          <  2:1289390/100‑1 (MQ=255)
   gCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTggg                                                                                          >  2:5527/1‑100 (MQ=255)
   gCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTggg                                                                                          <  2:607283/100‑1 (MQ=255)
      ggCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTc                                                                                       <  2:114518/100‑1 (MQ=255)
       gCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCg                                                                                      <  1:733409/100‑1 (MQ=255)
            ttttCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGc                                                                                 >  1:1018497/1‑100 (MQ=255)
               tCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTgg                                                                              <  1:1258435/100‑1 (MQ=255)
                  aTGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCt                                                                           <  2:952971/100‑1 (MQ=255)
                   tgctgcTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTc                                                                          <  2:379505/100‑1 (MQ=255)
                    gctgctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCa                                                                         <  2:1167650/100‑1 (MQ=255)
                        ctCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGtt                                                                     >  1:989203/1‑100 (MQ=255)
                         tCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTg                                                                    >  1:1231366/1‑100 (MQ=255)
                          cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa                                                                   >  2:1058852/1‑100 (MQ=255)
                          cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa                                                                   >  2:1167049/1‑100 (MQ=255)
                          cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGa                                                                   >  2:228292/1‑100 (MQ=255)
                          cATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGGTTGGGTTCGAACACTATCCTCAGGTTGa                                                                   >  2:169330/1‑100 (MQ=255)
                               atatCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGaa                                                              <  2:372754/100‑1 (MQ=255)
                                 atCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAat                                                            >  2:309888/1‑100 (MQ=255)
                                 atCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAat                                                            <  2:954214/100‑1 (MQ=255)
                                   cGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAatat                                                          >  2:1065098/1‑100 (MQ=255)
                                    gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc                                                         >  1:621628/1‑100 (MQ=255)
                                    gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc                                                         >  1:33297/1‑100 (MQ=255)
                                    gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc                                                         <  1:152708/100‑1 (MQ=255)
                                    gAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATc                                                         >  2:788770/1‑100 (MQ=255)
                                       ttACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGcc                                                      <  2:600570/100‑1 (MQ=255)
                                        tACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCt                                                     >  2:356967/1‑100 (MQ=255)
                                          cAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTgg                                                   <  1:232348/100‑1 (MQ=255)
                                             tGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGc                                                >  2:82987/1‑100 (MQ=255)
                                               atatACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCtt                                              <  1:788775/100‑1 (MQ=255)
                                                tataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCttt                                             <  1:832813/100‑1 (MQ=255)
                                                tataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCttt                                             <  2:621624/100‑1 (MQ=255)
                                                 ataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCtttt                                            <  1:713036/100‑1 (MQ=255)
                                                 ataCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCtttt                                            >  2:177834/1‑100 (MQ=255)
                                                  taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg                                           <  1:1164593/100‑1 (MQ=255)
                                                  taCACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTg                                           >  2:347477/1‑100 (MQ=255)
                                                    cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc                                         <  2:1018488/100‑1 (MQ=255)
                                                    cacaTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTc                                         <  1:1201225/100‑1 (MQ=255)
                                                      caTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCa                                       <  1:467660/100‑1 (MQ=255)
                                                       aTGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCAt                                      <  1:1047367/100‑1 (MQ=255)
                                                             tGCCTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGaa                                >  2:70001/1‑100 (MQ=255)
                                                                cTCCCTGCAAAGAAGAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCt                             >  2:421500/1‑100 (MQ=255)
                                                                 tCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCTATCTGGAACCt                             >  2:511266/1‑99 (MQ=255)
                                                                         aagaagAGACTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGc                    <  1:1065108/100‑1 (MQ=255)
                                                                              gagaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAAc               <  1:1106873/100‑1 (MQ=255)
                                                                                gaCTAACATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGc             >  2:870503/1‑100 (MQ=255)
                                                                                    aaCATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGa         >  1:932141/1‑100 (MQ=255)
                                                                                     aCATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGAc        <  1:356970/100‑1 (MQ=255)
                                                                                      cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa       >  1:102682/1‑100 (MQ=255)
                                                                                      cATTCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACa       >  1:1226516/1‑100 (MQ=255)
                                                                                         tCGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcc    <  1:831477/100‑1 (MQ=255)
                                                                                          cGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGcc    <  2:1005354/99‑1 (MQ=255)
                                                                                          cGAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCt   >  1:318874/1‑100 (MQ=255)
                                                                                           gAATAGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTc  <  2:932133/100‑1 (MQ=255)
                                                                                               |                                                                                               
TCAGCGGGCTATTTTTCGATGCTGCTCATAAATATCGAATTACAGTGATATACACATGTCTTGCCTCCCTGCAAAGAAGAGACTAACATTCGAATTGTTTGGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGTCCATCTGGAACCTCGCGAATGCTTAACGCCAGACAGCCTC  >  NC_000913/2887257‑2887447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: