| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,017,118 | C→A | R353R (CGC→CGA) | ygfK → | putative Fe‑S subunit oxidoreductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,017,118 | 0 | C | A | 93.8% | 149.3 / 0.1 | 48 | R353R (CGC→CGA) | ygfK | putative Fe‑S subunit oxidoreductase subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (25/20); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACT > NC_000913/3017023‑3017211 | aTCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCt > 1:453833/1‑100 (MQ=255) aacaaTAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTg > 2:256210/1‑100 (MQ=255) aacaaTAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTg < 2:927823/100‑1 (MQ=255) caaTAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGAc > 2:828469/1‑100 (MQ=255) aaaGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCa < 1:1230527/100‑1 (MQ=255) cgcACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTg > 2:431009/1‑100 (MQ=255) cACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGcc > 2:950986/1‑100 (MQ=255) gCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCAtt > 2:159905/1‑100 (MQ=255) ggTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGTAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCtt > 1:834359/1‑100 (MQ=255) gAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTAtt > 1:954260/1‑100 (MQ=255) agagATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGTAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTcc < 2:834353/100‑1 (MQ=255) gaTGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCggt < 1:1188090/100‑1 (MQ=255) atatGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGc < 1:119766/100‑1 (MQ=255) tatGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGcc < 2:708820/100‑1 (MQ=255) tGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAg > 1:89613/1‑100 (MQ=255) tGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAg < 2:12114/100‑1 (MQ=255) cAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCa < 1:76047/100‑1 (MQ=255) cAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCa < 2:132991/100‑1 (MQ=255) cAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCa > 2:1079611/1‑100 (MQ=255) ccGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTg < 2:1173913/100‑1 (MQ=255) gTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGAc < 2:727332/100‑1 (MQ=255) gcTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTa > 1:304489/1‑98 (MQ=255) gTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgc < 1:1024422/100‑1 (MQ=255) gTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgc > 1:158998/1‑100 (MQ=255) ttCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgcg < 2:948904/100‑1 (MQ=255) ttCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcgcg < 2:472472/100‑1 (MQ=255) cccGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGat < 1:950994/100‑1 (MQ=255) cccGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGat < 1:6365/100‑1 (MQ=255) acGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTTACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCcg > 2:790972/2‑95 (MQ=255) cGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGatat < 2:750348/100‑1 (MQ=255) cGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGatat < 2:959205/100‑1 (MQ=255) ctctCCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATAttt > 2:58073/1‑100 (MQ=255) ctcCATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATAttttt < 2:1270916/100‑1 (MQ=255) ccATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGa > 2:472104/1‑100 (MQ=255) ccATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGa < 1:803541/100‑1 (MQ=255) cATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGAt > 2:1928/1‑100 (MQ=255) cATCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGAt > 1:14703/1‑100 (MQ=255) tCAATGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATAc > 2:1205716/1‑100 (MQ=255) aaTGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAg < 2:158996/100‑1 (MQ=255) aTGTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAgg > 1:5216/1‑100 (MQ=255) gTTGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTa > 2:702483/1‑100 (MQ=255) tGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTAtt > 2:1145408/1‑100 (MQ=255) tGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTAtt > 2:1047347/1‑100 (MQ=255) tGCAGCAGTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTAtt > 1:737893/1‑100 (MQ=255) cagcagTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCg > 2:394539/1‑100 (MQ=255) cagcagTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCg > 2:557948/1‑100 (MQ=255) gcagTTCTCTCTCGAGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGcc < 1:260054/100‑1 (MQ=255) ctctcGAGCCTTTGACGGCAAACTGCCTATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACt > 1:1027674/1‑100 (MQ=255) | ATCAACAATAAAGGCGCACTGCCTGGTGAAGAGATGTATATGTCAGGCCGTGCGCTGTTCCCGCTCTCCATCAATGTTGCAGCAGTTCTCTCTCGCGCCTTTGACGGCAAACTGCCCATTTCTTATTCCGGTGGTGCCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACT > NC_000913/3017023‑3017211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |