Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,118,566 C→T A183T (GCG→ACG)  sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,118,5660CT95.2% 199.1 / 0.4 63A183T (GCG→ACG) sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (25/35);  total (26/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

ACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGAC  >  NC_000913/3118470‑3118660
                                                                                                |                                                                                              
aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCgg                                                                                             <  1:433399/100‑1 (MQ=255)
aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCgg                                                                                             >  1:1176482/1‑100 (MQ=255)
 ctctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGa                                                                                            >  1:562887/1‑100 (MQ=255)
 ctctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGa                                                                                            >  1:679296/1‑100 (MQ=255)
   ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACa                                                                                          <  1:499936/100‑1 (MQ=255)
   ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACa                                                                                          <  1:170962/100‑1 (MQ=255)
    ttCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAg                                                                                         <  1:957039/100‑1 (MQ=255)
     tCCGGTGCCAGATCTATTTGCTTATACAGCGAGTCTACGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGc                                                                                        <  2:1052650/100‑1 (MQ=255)
       cGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTg                                                                                      <  1:418799/100‑1 (MQ=255)
         acgctcttccGATCTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGtt                                                                                    <  2:650518/91‑1 (MQ=255)
            ccAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACt                                                                                 >  1:54289/1‑100 (MQ=255)
             cAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTCCTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTg                                                                                <  2:170162/100‑1 (MQ=255)
             cAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTg                                                                                >  2:490658/1‑100 (MQ=255)
               gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGa                                                                              <  2:150584/100‑1 (MQ=255)
                 tCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACg                                                                            <  1:346471/100‑1 (MQ=255)
                  ctaTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGt                                                                           >  1:883310/3‑100 (MQ=255)
                   gATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGtt                                                                          >  2:367830/1‑100 (MQ=255)
                     tttGC‑TATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTAcc                                                                       <  2:212797/100‑1 (MQ=255)
                             taCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGaaa                                                                <  2:1011634/100‑1 (MQ=255)
                             taCAGCGAGTCGAAGCGTTTACTCTCAATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGaaa                                                                >  1:795114/1‑100 (MQ=255)
                             taCAGCGAGTCGAAGCGTTTACTCTCAATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGaaa                                                                >  1:795314/1‑100 (MQ=255)
                              acggCGAGTCGAAGCGTTTACTCTCGCTCACCGAGGAGAACGTCAGAACAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAAc                                                               <  2:1087008/97‑1 (MQ=255)
                              aaaGCGAGTAGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAATCATGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAAc                                                               <  2:1086990/98‑1 (MQ=255)
                                  cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCa                                                           <  1:1049741/100‑1 (MQ=255)
                                    aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAtt                                                         <  2:562883/100‑1 (MQ=255)
                                      tCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGc                                                       <  2:883303/100‑1 (MQ=255)
                                       cGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCt                                                      <  1:315416/100‑1 (MQ=255)
                                         aaGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCttt                                                    <  1:125173/100‑1 (MQ=255)
                                                tACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCACTGCTTTTCCTg                                               >  2:603668/1‑98 (MQ=255)
                                                tACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTc                                             >  1:1304515/1‑100 (MQ=255)
                                                    ctcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCg                                         >  1:802373/1‑100 (MQ=255)
                                                     tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg                                        >  2:992301/1‑100 (MQ=255)
                                                     tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg                                        <  2:991539/100‑1 (MQ=255)
                                                     tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg                                        <  1:67304/100‑1 (MQ=255)
                                                       gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                      >  1:1217701/1‑100 (MQ=255)
                                                        aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGc                                     <  1:1146239/100‑1 (MQ=255)
                                                             cGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGcc                                <  2:463282/100‑1 (MQ=255)
                                                              gaggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGGCGTTAGCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCa                               <  1:367833/100‑1 (MQ=255)
                                                              gaggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCa                               >  2:1274233/1‑100 (MQ=255)
                                                                ggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt                             >  1:416656/1‑100 (MQ=255)
                                                                ggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt                             >  2:907403/1‑100 (MQ=255)
                                                                   gaACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACTAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCt                          >  2:1021649/1‑100 (MQ=255)
                                                                    aaCGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCtt                         >  1:459666/1‑100 (MQ=255)
                                                                      cGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgc                       <  1:907410/100‑1 (MQ=255)
                                                                       gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcn                      <  1:59114/100‑2 (MQ=255)
                                                                       gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                      >  2:1285807/1‑100 (MQ=255)
                                                                       gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                      >  1:756461/1‑100 (MQ=255)
                                                                       gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                      <  1:423513/100‑1 (MQ=255)
                                                                       gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                      <  2:679292/100‑1 (MQ=255)
                                                                        tCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                     >  2:656434/1‑100 (MQ=255)
                                                                         cAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcgc                     <  2:1025509/99‑1 (MQ=255)
                                                                         cAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                    <  1:634538/100‑1 (MQ=255)
                                                                         cAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGcgcg                    <  1:393354/100‑1 (MQ=255)
                                                                          aGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGt                   >  2:1141243/1‑100 (MQ=255)
                                                                           gACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTc                  >  1:640902/1‑100 (MQ=255)
                                                                            aCAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                 <  2:309854/100‑1 (MQ=255)
                                                                             cAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCt                <  2:1052177/100‑1 (MQ=255)
                                                                                   tGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGc          >  1:837698/1‑100 (MQ=255)
                                                                                     ttCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTa        <  2:456131/100‑1 (MQ=255)
                                                                                       cTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTaaa      >  2:357564/1‑100 (MQ=255)
                                                                                        tGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAAc     <  1:1169286/100‑1 (MQ=255)
                                                                                           aTTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGac  <  1:470273/100‑1 (MQ=255)
                                                                                            ttCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACg    <  2:814936/97‑1 (MQ=255)
                                                                                                |                                                                                              
ACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGAC  >  NC_000913/3118470‑3118660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑