| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,190,398 | T→G | M173R (ATG→AGG) | yqiH → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,190,398 | 0 | T | G | 98.1% | 177.8 / NA | 53 | M173R (ATG→AGG) | yqiH | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (21/31); minor base A (0/1); total (21/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAA > NC_000913/3190301‑3190494 | ccAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGc < 1:646745/100‑1 (MQ=255)ccAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGc > 1:958695/1‑100 (MQ=255) aaGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTa < 2:51932/100‑1 (MQ=255) gCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATc < 1:628980/100‑1 (MQ=255) gCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATc < 1:511498/100‑1 (MQ=255) aTTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACg > 1:223315/1‑100 (MQ=255) tGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGtt < 2:140939/100‑1 (MQ=255) gAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTa > 1:359091/1‑100 (MQ=255) tAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCt > 2:335221/1‑100 (MQ=255) tAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCt > 2:112183/1‑100 (MQ=255) tAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCt > 2:41267/1‑100 (MQ=255) tAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCt < 1:824368/100‑1 (MQ=255) agaagaCGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCt < 2:134662/99‑1 (MQ=255) agaagaCGATGGGGTAATGACTTACGAAAAAGTAGAAATCATTCGTAGAAATGAAGGTTAGATCCGCTTTAACAATAAGAGGCCCTATCACGTTAAGc > 2:443215/1‑98 (MQ=255) aagaCGATTGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTggg < 2:621087/100‑1 (MQ=255) aagaCGATGTGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTggg < 2:1273558/100‑1 (MQ=255) agaCGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGt < 2:359088/100‑1 (MQ=255) agaCGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGt < 2:25712/100‑1 (MQ=255) agaCGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGt > 1:1086104/1‑100 (MQ=255) gaCGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGtt < 1:921974/100‑1 (MQ=255) aCGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTta < 2:480931/100‑1 (MQ=255) cGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTta > 1:1020719/1‑99 (MQ=255) gggTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCgg > 2:49400/1‑100 (MQ=255) aatAACGACTTACAAACAAGTTGAAATCACTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAAGCCTTATCACCTTACGCTCGGTTATATCgg < 2:286460/97‑1 (MQ=255) aCTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCAATCCGCTTTAACAATAAGAGGCCTTATCACGGTACGCTGGATTATATCGGAACaaa > 2:1024348/1‑98 (MQ=255) ttACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATggg < 2:141525/100‑1 (MQ=255) ttACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATggg < 1:335224/100‑1 (MQ=255) aCGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGt > 1:510449/1‑100 (MQ=255) aaaaGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACg < 1:385735/100‑1 (MQ=255) aGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATg < 2:928381/100‑1 (MQ=255) aGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATg < 1:519019/100‑1 (MQ=255) ttGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGtt < 1:1144550/100‑1 (MQ=255) ttGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGtt < 2:294553/100‑1 (MQ=255) ttGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGtt < 1:1245690/100‑1 (MQ=255) tGAAATCATTCGTAGAAATTACGTTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTa > 2:339810/1‑100 (MQ=255) gAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTAc > 2:864346/1‑100 (MQ=255) gAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTAc < 1:139000/100‑1 (MQ=255) aaaTCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTAcc < 1:418009/100‑1 (MQ=255) aaaTCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTAcc < 1:737131/100‑1 (MQ=255) tCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACg < 2:1155440/100‑1 (MQ=255) cGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGc > 1:966442/1‑100 (MQ=255) cGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGc > 1:1197571/1‑100 (MQ=255) gAAATGACGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAg > 1:720354/1‑100 (MQ=255) aCGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAAt > 1:152440/1‑100 (MQ=255) cGGTTCGATCCGCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATg > 1:624978/1‑100 (MQ=255) ggTTCGATCCGCTTTAACNATAAGAGGCCTTNTCANGTTACGCNGGGTNANATNGGAANAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATgg < 1:868311/100‑1 (MQ=255) ttggATCCTCTTTAAAAATAAGAGGCCTTGTCACGCTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTCCCACAGACGCAAAGCTTAATgg < 2:402867/95‑1 (MQ=255) gCTTTAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCttt > 1:57888/1‑100 (MQ=255) tttAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTa < 1:21336/100‑1 (MQ=255) ttAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAg > 2:664425/1‑100 (MQ=255) tAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGc < 2:333981/100‑1 (MQ=255) tAACAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGc < 1:864352/100‑1 (MQ=255) aaCAATAAGAGGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCt < 1:810039/100‑1 (MQ=255) agagGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCaaa > 1:307708/1‑100 (MQ=255) | CCAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAA > NC_000913/3190301‑3190494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |