Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,244,928 | T→C | intergenic (‑187/‑296) | uxaC ← / → exuT | uronate isomerase/hexuronate transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,244,928 | 0 | T | C | 98.3% | 207.6 / ‑4.2 | 60 | intergenic (‑187/‑296) | uxaC/exuT | uronate isomerase/hexuronate transporter |
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (30/29); total (31/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 |
CGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAC > NC_000913/3244833‑3245023 | cGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATg > 2:504068/1‑100 (MQ=255) gTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGt > 2:465251/1‑100 (MQ=255) tGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTc < 1:1118309/100‑1 (MQ=255) gTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCt > 2:257601/1‑100 (MQ=255) gTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCt > 1:302561/1‑100 (MQ=255) gATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACaa > 1:1069961/1‑100 (MQ=255) gATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACaa > 2:177430/1‑100 (MQ=255) tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTc > 1:613296/1‑100 (MQ=255) aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTcc > 2:1244109/1‑100 (MQ=255) aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTccc < 1:670011/100‑1 (MQ=255) gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCcaca > 1:510505/1‑100 (MQ=255) tAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgt > 1:246544/1‑100 (MQ=255) tAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgt > 2:370028/1‑100 (MQ=255) aaaaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgtg < 2:1010492/100‑1 (MQ=255) aaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGAt < 1:148158/100‑1 (MQ=255) aaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGAt > 1:1206056/1‑100 (MQ=255) aaaaTTACTCTCAAAGTGGTAAATCTAGCTGCAGGCCGCGCCAGTACTGGATTTGCTGTCGTCAGGCACTGTCCCTACAAACATTCCCACATTTGTGAt > 2:756306/1‑99 (MQ=255) aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGc < 1:170913/100‑1 (MQ=255) aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGc < 2:246541/100‑1 (MQ=255) aTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGct < 1:695962/100‑1 (MQ=255) aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc > 2:900210/1‑100 (MQ=255) aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc > 2:336446/1‑100 (MQ=255) aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc > 1:792932/1‑100 (MQ=255) tctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAc < 2:1039832/100‑1 (MQ=255) ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc < 1:370031/100‑1 (MQ=255) ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc > 1:1010837/1‑100 (MQ=255) ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATCTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc > 2:245799/1‑100 (MQ=255) ctcAAAGTGGTAAATCTCGCAGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc > 2:800155/1‑100 (MQ=255) tcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt > 1:441788/1‑100 (MQ=255) tcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt > 2:1231155/1‑100 (MQ=255) cAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtt < 1:142088/100‑1 (MQ=255) tAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGtt > 1:1051/1‑100 (MQ=255) acaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg < 2:792927/98‑1 (MQ=255) aaaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg < 2:527571/100‑1 (MQ=255) aTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTa < 2:168391/100‑1 (MQ=255) tctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt < 1:224666/100‑1 (MQ=255) ctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATg > 2:111633/1‑100 (MQ=255) cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACaa > 2:680484/1‑100 (MQ=255) gCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt < 2:1183145/100‑1 (MQ=255) ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt < 2:231673/100‑1 (MQ=255) ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt > 2:235274/1‑100 (MQ=255) gCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATc < 1:32726/100‑1 (MQ=255) cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt > 1:1274313/1‑100 (MQ=255) cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt < 2:439359/100‑1 (MQ=255) cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt < 2:439820/100‑1 (MQ=255) ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg < 1:71530/100‑1 (MQ=255) ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg < 1:800160/100‑1 (MQ=255) aGTACTTCCCTTGCTGTCGTAAGGCAATGTCCTTATGAAGATTTGCTAATTTGTGATGGCTCTCCATTTTTAATCTTTTATGAGATGTTATCtttacgcc < 2:1089907/100‑6 (MQ=255) aGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGcc > 1:869534/1‑100 (MQ=255) tACTGGACTAGCTGCCGTCAGGCAATGTCGCTACAAATATTCCCACATTTGTTATAGCGCTCACCTTTTAAAGTTGTGTTACAAGTTATCGTTcctcag < 2:530689/99‑6 (MQ=255) cattGCTGTCGTCGGGTAATGTCCCTACAAATATTCCCACATATGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCaaa > 1:882204/3‑100 (MQ=255) tGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa < 1:331283/100‑1 (MQ=255) tGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa < 1:1244120/100‑1 (MQ=255) gCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAATTcct > 1:62927/1‑98 (MQ=255) tGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATaa > 1:352267/1‑100 (MQ=255) gtcgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAg < 2:195421/100‑1 (MQ=255) gtcgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAAATAAAAAAAAAATAATATTTCTGCCGTCGCAAATCATAAg < 2:613292/100‑1 (MQ=255) gtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg > 1:366963/1‑100 (MQ=255) gtcAGGCAATGTCCCTACAAATATCCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt < 1:111635/98‑1 (MQ=255) cAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc < 1:1015786/100‑1 (MQ=255) | CGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAC > NC_000913/3244833‑3245023 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |