Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,244,928 T→C intergenic (‑187/‑296) uxaC ← / → exuT uronate isomerase/hexuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,244,9280TC98.3% 207.6 / ‑4.2 60intergenic (‑187/‑296)uxaC/exuTuronate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (30/29);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

CGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAC  >  NC_000913/3244833‑3245023
                                                                                               |                                                                                               
cGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATg                                                                                             >  2:504068/1‑100 (MQ=255)
 gTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGt                                                                                            >  2:465251/1‑100 (MQ=255)
  tGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTc                                                                                           <  1:1118309/100‑1 (MQ=255)
     gTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCt                                                                                        >  2:257601/1‑100 (MQ=255)
     gTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCt                                                                                        >  1:302561/1‑100 (MQ=255)
         gATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACaa                                                                                    >  1:1069961/1‑100 (MQ=255)
         gATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACaa                                                                                    >  2:177430/1‑100 (MQ=255)
               tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTc                                                                              >  1:613296/1‑100 (MQ=255)
                aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTcc                                                                             >  2:1244109/1‑100 (MQ=255)
                 aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTccc                                                                            <  1:670011/100‑1 (MQ=255)
                    gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCcaca                                                                         >  1:510505/1‑100 (MQ=255)
                         tAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgt                                                                    >  1:246544/1‑100 (MQ=255)
                         tAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgt                                                                    >  2:370028/1‑100 (MQ=255)
                          aaaaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgtg                                                                   <  2:1010492/100‑1 (MQ=255)
                            aaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGAt                                                                 <  1:148158/100‑1 (MQ=255)
                            aaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGAt                                                                 >  1:1206056/1‑100 (MQ=255)
                             aaaaTTACTCTCAAAGTGGTAAATCTAGCTGCAGGCCGCGCCAGTACTGGATTTGCTGTCGTCAGGCACTGTCCCTACAAACATTCCCACATTTGTGAt                                                                 >  2:756306/1‑99 (MQ=255)
                               aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGc                                                              <  1:170913/100‑1 (MQ=255)
                               aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGc                                                              <  2:246541/100‑1 (MQ=255)
                                aTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGct                                                             <  1:695962/100‑1 (MQ=255)
                                   aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc                                                          >  2:900210/1‑100 (MQ=255)
                                   aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc                                                          >  2:336446/1‑100 (MQ=255)
                                   aCTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc                                                          >  1:792932/1‑100 (MQ=255)
                                     tctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAc                                                        <  2:1039832/100‑1 (MQ=255)
                                      ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                       <  1:370031/100‑1 (MQ=255)
                                      ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                       >  1:1010837/1‑100 (MQ=255)
                                      ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATCTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                       >  2:245799/1‑100 (MQ=255)
                                      ctcAAAGTGGTAAATCTCGCAGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                       >  2:800155/1‑100 (MQ=255)
                                       tcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt                                                      >  1:441788/1‑100 (MQ=255)
                                       tcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt                                                      >  2:1231155/1‑100 (MQ=255)
                                        cAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtt                                                     <  1:142088/100‑1 (MQ=255)
                                                tAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGtt                                             >  1:1051/1‑100 (MQ=255)
                                                 acaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg                                            <  2:792927/98‑1 (MQ=255)
                                                 aaaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg                                            <  2:527571/100‑1 (MQ=255)
                                                   aTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTa                                          <  2:168391/100‑1 (MQ=255)
                                                    tctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                         <  1:224666/100‑1 (MQ=255)
                                                     ctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATg                                        >  2:111633/1‑100 (MQ=255)
                                                         cTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACaa                                    >  2:680484/1‑100 (MQ=255)
                                                           gCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                                  <  2:1183145/100‑1 (MQ=255)
                                                              ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt                               <  2:231673/100‑1 (MQ=255)
                                                              ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt                               >  2:235274/1‑100 (MQ=255)
                                                               gCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATc                              <  1:32726/100‑1 (MQ=255)
                                                                 cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                            >  1:1274313/1‑100 (MQ=255)
                                                                 cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                            <  2:439359/100‑1 (MQ=255)
                                                                 cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                            <  2:439820/100‑1 (MQ=255)
                                                                     ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg                        <  1:71530/100‑1 (MQ=255)
                                                                     ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg                        <  1:800160/100‑1 (MQ=255)
                                                                       aGTACTTCCCTTGCTGTCGTAAGGCAATGTCCTTATGAAGATTTGCTAATTTGTGATGGCTCTCCATTTTTAATCTTTTATGAGATGTTATCtttacgcc                      <  2:1089907/100‑6 (MQ=255)
                                                                       aGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGcc                      >  1:869534/1‑100 (MQ=255)
                                                                         tACTGGACTAGCTGCCGTCAGGCAATGTCGCTACAAATATTCCCACATTTGTTATAGCGCTCACCTTTTAAAGTTGTGTTACAAGTTATCGTTcctcag                     <  2:530689/99‑6 (MQ=255)
                                                                               cattGCTGTCGTCGGGTAATGTCCCTACAAATATTCCCACATATGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCaaa              >  1:882204/3‑100 (MQ=255)
                                                                                  tGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa           <  1:331283/100‑1 (MQ=255)
                                                                                  tGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa           <  1:1244120/100‑1 (MQ=255)
                                                                                   gCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAATTcct          >  1:62927/1‑98 (MQ=255)
                                                                                     tGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATaa        >  1:352267/1‑100 (MQ=255)
                                                                                      gtcgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAg       <  2:195421/100‑1 (MQ=255)
                                                                                      gtcgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAAATAAAAAAAAAATAATATTTCTGCCGTCGCAAATCATAAg       <  2:613292/100‑1 (MQ=255)
                                                                                         gtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg    >  1:366963/1‑100 (MQ=255)
                                                                                         gtcAGGCAATGTCCCTACAAATATCCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt      <  1:111635/98‑1 (MQ=255)
                                                                                           cAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc  <  1:1015786/100‑1 (MQ=255)
                                                                                               |                                                                                               
CGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAC  >  NC_000913/3244833‑3245023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: