| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 339,856 | T→C | intergenic (+113/‑309) | yahG → / → yahI | DUF1116 family protein/carbamate kinase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 339,856 | 0 | T | C | 92.7% | 115.0 / 1.1 | 42 | intergenic (+113/‑309) | yahG/yahI | DUF1116 family protein/carbamate kinase‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (16/22); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01 | |||||||||||
ATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGC > NC_000913/339762‑339951 | aTGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACgcggc < 2:324502/100‑1 (MQ=255) gAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGcc < 1:953578/100‑1 (MQ=255) ccgatcTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCt < 2:568062/95‑1 (MQ=255) aaCGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGTCGGACGCGGCGTGAACGCCt < 2:794355/100‑1 (MQ=255) aaCGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCt < 1:932945/100‑1 (MQ=255) aaCGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCt < 2:280999/100‑1 (MQ=255) cTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATcc < 1:636934/100‑1 (MQ=255) tATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCgg > 1:97816/1‑100 (MQ=255) aTCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTCCCGGACGCGGCGTGAACGCCTTATcc > 2:87650/1‑97 (MQ=255) cttACGAATAGCGCAAGAATCTGTAGGCCTGATAAGTGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGa < 2:337917/98‑1 (MQ=11) tACGAATGGCGCAAGAATCTTTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCAGCGTGAACGCCTTATCCGGCCTACGa < 2:337865/98‑1 (MQ=255) tACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAAt < 1:1297363/100‑1 (MQ=255) aCGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATg < 1:162027/100‑1 (MQ=255) aaTGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGgcg > 2:1264721/1‑100 (MQ=255) ggCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGgcg > 2:1076892/1‑97 (MQ=38) aGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCGAGAATCt > 2:340387/1‑100 (MQ=9) aTCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTa > 1:698617/1‑100 (MQ=255) tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGc < 1:566659/100‑1 (MQ=255) tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGAAGGc > 2:278964/1‑100 (MQ=255) tAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCt < 1:637670/100‑1 (MQ=255) ggCCGGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGa < 2:903292/100‑1 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATa < 1:558139/100‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCGAGAATCTGAAGGCCGGCTAAGc > 2:444842/1‑100 (MQ=11) gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAg > 1:1050699/1‑99 (MQ=255) naaGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGt > 1:385087/2‑100 (MQ=255) gCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgc > 2:686355/1‑100 (MQ=255) gCGTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgc < 1:1264732/100‑1 (MQ=255) gTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgcgc > 1:1088762/1‑100 (MQ=255) gTAGCGCATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAgcgc < 1:779121/100‑1 (MQ=255) cgcATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCa > 1:964991/1‑100 (MQ=255) gcATCAGGCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAg < 1:87652/100‑1 (MQ=255) ggCATTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAttt < 1:1228367/100‑1 (MQ=255) aTTTGTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGcc < 1:990928/100‑1 (MQ=255) gTCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTGGGCCTGTTAACCGTAGGGCATCAGGCATTTGccacc > 2:1133486/1‑99 (MQ=14) tCACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCcaccac < 1:686359/100‑1 (MQ=255) cACCATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACt < 1:104687/100‑1 (MQ=255) cATTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGcc > 1:1186182/1‑100 (MQ=255) aTTGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGccacc > 2:754308/1‑93 (MQ=25) tGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCg < 2:875027/98‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCGTCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCAGGa > 2:1119951/2‑100 (MQ=11) gCCGGACGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGACATTTGCCACCACTGCCGGAt < 1:1195970/100‑1 (MQ=255) cGGAGGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATgc < 2:1203810/100‑1 (MQ=255) | ATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGC > NC_000913/339762‑339951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |