Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,544	G→C	P104A (CCT→GCT)	chiA ←	periplasmic endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,544	0	G	C	100.0%	230.0 / NA	63	P104A (CCT→GCT)	chiA	periplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (30/33);  total (30/33)

CCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAA  >  NC_000913/3469447‑3469640                                                                                                  |                                                                                                 cgatcttgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCg                                                                                                <  2:240574/94‑1 (MQ=255)  ccTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGt                                                                                               >  1:334452/1‑100 (MQ=255)       tgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAATCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttg                                                                                          <  2:588202/100‑1 (MQ=255)       tgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCgagatag                                                                                          >  1:240576/1‑94 (MQ=255)       tgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttg                                                                                          >  2:435440/1‑100 (MQ=255)       tgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTattt                                                                                           >  2:864942/1‑99 (MQ=255)         tttATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaa                                                                                        <  1:786437/100‑1 (MQ=255)           tATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaatt                                                                                      <  2:1062968/100‑1 (MQ=255)           tATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaatt                                                                                      <  1:17028/100‑1 (MQ=255)            aTTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaattt                                                                                     >  1:893982/1‑100 (MQ=255)              tCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaatttga                                                                                   <  2:1248441/100‑1 (MQ=255)              tCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGCATGCAGCCGTatttgaatttga                                                                                   <  2:755099/100‑1 (MQ=255)               ccAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTAtttgaatttgaa                                                                                  >  1:720429/1‑100 (MQ=255)               ccAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTAtttgaatttgaa                                                                                  >  2:778753/1‑100 (MQ=255)               ccAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCCGCGTATTGGATGCAGCCGTAtttgaatttgaa                                                                                  <  1:1237837/100‑1 (MQ=255)                      aCTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATg                                                                           <  1:393481/100‑1 (MQ=255)                       cTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGa                                                                          >  1:539875/1‑100 (MQ=255)                        tACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAg                                                                         <  1:516183/100‑1 (MQ=255)                            gaagaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGc                                                                     >  1:1244089/1‑100 (MQ=255)                             aagaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGcc                                                                    <  1:738203/100‑1 (MQ=255)                               gaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGc                                                                  <  2:1165500/100‑1 (MQ=255)                               gaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGc                                                                  <  2:1165682/100‑1 (MQ=255)                                aaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGct                                                                 <  1:1074991/100‑1 (MQ=255)                                 aTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGctc                                                                >  1:314767/1‑100 (MQ=255)                                 aTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTCGAATTTGAAGAAGATGAGCTTCCGctc                                                                >  1:338610/1‑100 (MQ=255)                                       cGGAACCGTGCACTGTGCTGGTGTAGCAGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGACGATGAGCTGCCGCTCTTTTCg                                                          >  2:259941/1‑100 (MQ=255)                                       cGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCg                                                          >  1:141138/1‑100 (MQ=255)                                        ggAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGc                                                         >  1:945463/1‑100 (MQ=255)                                         gAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCa                                                        >  2:985399/1‑100 (MQ=255)                                          aaCAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAg                                                       <  1:812710/100‑1 (MQ=255)                                          aaCAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAg                                                       <  2:639326/100‑1 (MQ=255)                                          aaCAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAg                                                       <  2:1031617/100‑1 (MQ=255)                                           aCAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAgg                                                      >  2:1059018/1‑100 (MQ=255)                                               tGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAg                                                  <  2:357368/100‑1 (MQ=255)                                               tGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAg                                                  <  1:1211882/100‑1 (MQ=255)                                                gCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaaac                                                  <  2:191430/99‑2 (MQ=255)                                                gCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAgt                                                 >  2:485690/1‑100 (MQ=255)                                                 cccTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAgtg                                                <  1:994933/100‑1 (MQ=255)                                                   cTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTa                                              >  1:708843/1‑100 (MQ=255)                                                     gtgCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTAtt                                            <  1:1202895/100‑1 (MQ=255)                                                      tgCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTg                                           >  1:1157932/1‑100 (MQ=255)                                                       gCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGc                                          <  1:144771/100‑1 (MQ=255)                                                        cTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGACGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGcc                                         <  1:586522/100‑1 (MQ=255)                                                           gtgtAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaaa                                      >  2:575126/1‑100 (MQ=255)                                                            tgtAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAAc                                     <  2:1010467/100‑1 (MQ=255)                                                            tgtAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAAc                                     <  1:1199473/100‑1 (MQ=255)                                                             gtAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACt                                    >  1:1016923/1‑100 (MQ=255)                                                             gtAGCCGAACCACCATTAGCCGGCGTATTGGATGCAGCCGCTTTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACt                                    >  2:711780/1‑100 (MQ=255)                                                               aGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGa                                  >  1:399477/1‑100 (MQ=255)                                                               aGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGa                                  >  2:767478/1‑100 (MQ=255)                                                               aGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGa                                  >  1:297259/1‑100 (MQ=255)                                                                  cGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTa                               >  1:1290949/1‑100 (MQ=255)                                                                        gCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTca                         >  1:861072/1‑100 (MQ=255)                                                                            ttAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagcag                     <  2:673989/100‑1 (MQ=255)                                                                                   gCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGc              <  2:731189/100‑1 (MQ=255)                                                                                      tATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGt           <  2:893975/100‑1 (MQ=255)                                                                                       aTTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTa          >  1:247786/1‑100 (MQ=255)                                                                                        ttGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTAc         <  2:609903/100‑1 (MQ=255)                                                                                         tGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACg        <  2:709835/100‑1 (MQ=255)                                                                                          ggATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGc       >  2:105500/1‑100 (MQ=255)                                                                                            aTGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCtt     <  2:539871/100‑1 (MQ=255)                                                                                            aTGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCtt     <  1:1082945/100‑1 (MQ=255)                                                                                               cAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTaa  >  1:1138467/1‑100 (MQ=255)                                                                                                  |                                                                                                 CCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAA  >  NC_000913/3469447‑3469640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑