| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,608,471 | T→A | L674Q (CTG→CAG) | zntA → | zinc, cobalt and lead efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,608,471 | 0 | T | A | 97.7% | 150.8 / ‑5.0 | 44 | L674Q (CTG→CAG) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (26/17); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG > NC_000913/3608380‑3608567 | gCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCAcgcg > 1:531225/1‑100 (MQ=255)gCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCAcgcg > 1:512942/1‑100 (MQ=255) aaTGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGcc > 1:1283657/1‑100 (MQ=255) ggTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTc > 2:1081429/1‑100 (MQ=255) gCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGc > 1:19007/1‑100 (MQ=255) gCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGAGCCAATGATTGAACAGGCACGCGCCACTCACGc > 2:904676/1‑100 (MQ=255) cGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGcc < 2:1251329/100‑1 (MQ=255) ggCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCa > 2:966503/1‑100 (MQ=255) cacaGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAat < 1:216779/100‑1 (MQ=255) aCGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCg < 1:1255742/100‑1 (MQ=255) gcgcTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGa > 1:735520/1‑100 (MQ=255) cTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACa < 1:158789/100‑1 (MQ=255) tGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACAt < 1:633147/100‑1 (MQ=255) ggAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATc > 2:1052066/1‑100 (MQ=255) cgccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTAtt > 1:1302140/1‑100 (MQ=255) gcagcaTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCGCGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTgg < 2:725588/100‑1 (MQ=255) aTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTg > 2:1048304/1‑100 (MQ=255) ttAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa < 1:638304/100‑1 (MQ=255) tAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTgga < 1:881586/100‑3 (MQ=255) tAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGaa < 2:662289/100‑1 (MQ=255) aCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAg < 2:512938/100‑1 (MQ=255) cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg > 2:37844/1‑100 (MQ=255) cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg > 2:318307/1‑100 (MQ=255) cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg > 1:1035132/1‑100 (MQ=255) cccATAACAACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGACAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg < 2:1110382/100‑1 (MQ=255) cATAACCGCCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACGCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGa < 2:218642/100‑1 (MQ=255) cATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGa < 1:1029568/100‑1 (MQ=255) aaCCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCt > 1:286822/1‑100 (MQ=255) accaccTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCtt > 1:601564/1‑100 (MQ=255) caccTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATGTCCGCCAGAACTTCACTATTGCGCTGGGGCTAAAAGGGATCTTcc < 2:536765/100‑1 (MQ=255) accTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATGGCGCTGGGGCGGAGAGGGACCTTcc > 1:191935/1‑99 (MQ=255) cTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg < 2:1098977/100‑1 (MQ=255) cTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg > 1:366193/1‑100 (MQ=255) tGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGt > 2:840330/1‑100 (MQ=255) gcgcGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTc > 1:259311/1‑100 (MQ=255) gcgcGACCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTc > 1:1304024/1‑100 (MQ=255) cgGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcacc > 2:693482/1‑100 (MQ=255) cTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGc > 1:552086/1‑100 (MQ=255) aaTGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCCCTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAggg < 2:566614/100‑1 (MQ=255) aaTGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAggg < 2:775505/100‑1 (MQ=255) tGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGAt < 2:1283646/100‑1 (MQ=255) aTTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGa > 2:653236/1‑100 (MQ=255) gAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGAATCTTCCTCGTCACCACGCTGTTAGGGATGAc > 2:273615/1‑98 (MQ=255) aaCAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCgg > 2:939612/1‑100 (MQ=255) | GCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG > NC_000913/3608380‑3608567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |