Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,622,796 T→G L1202R (CTG→CGG)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,622,7960TG97.6% 105.9 / ‑3.5 42L1202R (CTG→CGG) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (25/16);  total (25/17)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

TGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGA  >  NC_000913/3622701‑3622890
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tgatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAt                                                                                            <  2:190780/100‑1 (MQ=25)
 gatgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTAtt                                                                                           >  1:129541/1‑100 (MQ=18)
  atgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTa                                                                                          >  1:709812/1‑100 (MQ=18)
   tgaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTAc                                                                                         <  2:215216/100‑1 (MQ=18)
    gaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACa                                                                                        <  2:537474/100‑1 (MQ=18)
    gaATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACa                                                                                        >  2:1095124/1‑100 (MQ=17)
        ggggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCg                                                                                    >  1:554967/1‑100 (MQ=18)
        ggggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCg                                                                                    >  1:244165/1‑100 (MQ=18)
          ggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGcc                                                                                  >  1:790779/1‑100 (MQ=17)
          ggCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGcc                                                                                  >  1:748701/1‑100 (MQ=18)
            cAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCAc                                                                                >  1:3202/1‑100 (MQ=18)
               ccTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGc                                                                             <  1:734407/100‑1 (MQ=18)
                  gctgAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtat                                                                          >  1:657762/1‑100 (MQ=18)
                     gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGGGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat                                                                       <  2:701004/100‑1 (MQ=255)
                     gaatgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCtattat                                                                       <  1:72020/100‑1 (MQ=18)
                       atgaaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGa                                                                     >  2:728607/1‑100 (MQ=18)
                          aaGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGAccc                                                                  >  1:178682/1‑100 (MQ=18)
                                  ccGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAgg                                                          >  1:1022203/1‑100 (MQ=18)
                                   cGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCa                                                            <  2:940885/97‑1 (MQ=17)
                                     cATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGc                                                       >  1:265168/1‑100 (MQ=18)
                                      aTCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCg                                                      >  1:73135/1‑100 (MQ=18)
                                       tCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGa                                                     <  1:583578/100‑1 (MQ=17)
                                         aGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGata                                                   <  1:285740/100‑1 (MQ=18)
                                          gCTGCAGCAGCTTATACGGCTGCTGGGGCAGCAGTATGATGAGGAGTCCGGACGGAATTACAACCGCCACCGCTCTTATGACCCGCGGCAGGAGCGata                                                   >  2:317525/1‑99 (MQ=11)
                                             gcagcaGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATc                                               >  1:1017011/1‑100 (MQ=18)
                                               agcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCa                                              <  2:270078/99‑1 (MQ=17)
                                               agcagcTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCAc                                             <  2:265165/100‑1 (MQ=18)
                                                        tCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATcc                                    >  2:43423/1‑100 (MQ=17)
                                                           ggCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGAt                                 >  1:1032740/1‑100 (MQ=14)
                                                           ggCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGAt                                 <  1:461007/100‑1 (MQ=14)
                                                                ccGGGGCAGCAGTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGAc                            <  2:1096649/100‑1 (MQ=14)
                                                                          agTATGATGAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAgggggg                  >  1:593125/1‑100 (MQ=25)
                                                                             atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg               >  1:135970/1‑100 (MQ=255)
                                                                             atgatgAGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATg               >  1:758828/1‑100 (MQ=255)
                                                                              tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATgg              <  2:193865/100‑1 (MQ=255)
                                                                              tgatgaGGAGTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATgg              >  1:230699/1‑100 (MQ=255)
                                                                                  gaggagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGGTGGAAcc          <  1:1201256/100‑1 (MQ=255)
                                                                                     gagTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGt       >  2:103129/1‑100 (MQ=255)
                                                                                      agTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTa      <  2:677177/100‑1 (MQ=255)
                                                                                       gTCCGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTAt     >  1:438490/1‑100 (MQ=255)
                                                                                          cGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGa  >  2:294974/1‑100 (MQ=255)
                                                                                          cGGCCGGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGa  <  2:709807/100‑1 (MQ=255)
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TGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGA  >  NC_000913/3622701‑3622890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: