| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,783,402 | A→G | V87A (GTC→GCC) | gpsA ← | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,783,402 | 0 | A | G | 97.7% | 151.8 / ‑4.3 | 45 | V87A (GTC→GCC) | gpsA | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (20/23); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
ACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAGGCACATC > NC_000913/3783308‑3783496 | aCGTCCGGTTTCCGCTTCCAGCCCGTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATgn < 2:1076551/100‑2 (MQ=255) cGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCCGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCACAGTCATGGc > 2:638129/1‑100 (MQ=255) cGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGc < 2:69972/100‑1 (MQ=255) cGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTggg < 1:62225/100‑1 (MQ=255) gTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTa < 2:982351/100‑1 (MQ=255) ttCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTacg > 2:862532/1‑100 (MQ=255) ttCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTacg < 1:731227/100‑1 (MQ=255) ttCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTac > 2:175325/1‑99 (MQ=255) cTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTacgacgac < 2:726819/100‑1 (MQ=255) gCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAat < 2:1101914/100‑1 (MQ=255) cTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATAtt > 2:1298245/1‑100 (MQ=255) tttGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTa < 2:384571/100‑1 (MQ=255) cGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACggctg < 2:336865/100‑1 (MQ=255) acCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCa > 1:494262/1‑100 (MQ=255) ccAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAg > 1:722665/1‑100 (MQ=255) gACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAgcgc < 1:996473/100‑1 (MQ=255) cgcgCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAg > 2:241097/1‑100 (MQ=255) cgcgCAGCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAg < 1:1119377/100‑1 (MQ=255) cgcATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTg < 2:1287336/100‑1 (MQ=255) gcATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTgg < 2:82753/100‑1 (MQ=255) aTCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCg < 2:884871/100‑1 (MQ=255) cAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCgag < 1:231014/100‑1 (MQ=255) ggACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGAt > 1:91810/1‑100 (MQ=255) cGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCTAGATCg < 2:106872/99‑1 (MQ=255) cATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCtt > 1:439340/1‑100 (MQ=255) tCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTc > 2:733328/1‑100 (MQ=255) cAGGGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCGGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCACAGTGGCGAGATCGCttt < 2:202608/98‑1 (MQ=255) aGTGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCaa > 1:1099970/1‑100 (MQ=255) tGGTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGa > 1:471847/1‑100 (MQ=255) ggTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGAt < 2:321714/100‑1 (MQ=255) gTTTAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATg > 1:870141/1‑100 (MQ=255) tAATCTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAg < 1:519820/100‑1 (MQ=255) cTGGCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTa > 1:630549/1‑100 (MQ=255) ggCGCAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATc > 2:347456/1‑100 (MQ=255) gcgcAGCACTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCg > 1:1073966/1‑100 (MQ=255) gcagcaCTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCggg < 1:811120/100‑1 (MQ=255) aCTTCACCAAAGGGATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAg > 1:884901/1‑100 (MQ=255) aCTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAg < 1:241099/100‑1 (MQ=255) aCTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAg < 2:602779/100‑1 (MQ=255) cTTCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAgg > 1:437001/1‑100 (MQ=255) ttCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAGGc < 1:1196699/100‑1 (MQ=255) ttCACCAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAGGc < 2:870135/100‑1 (MQ=255) ccAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAGGCACAt > 2:336084/1‑100 (MQ=255) cAAAGGCATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAGGCACATc > 2:501418/1‑100 (MQ=255) aaaGGCGTGACTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCAAGATCGCTTTCACGATGGAGCGTATCGGGAAAAGGCACATc > 2:1243654/1‑99 (MQ=255) | ACGTCCGGTTTCCGCTTCCAGCCCTTTGGTCGCCCACACCAGACGCGCATCAGGACGCATCAGTGGTTTAATCTGGCGCAGCACTTCACCAAAGACATGGCTGGGTACGACGACGAGAATATTACGGCTGGCTGCCAGCGCAGTGGCGAGATCGCTTTCAAGATGGAGCGTATCGGGAAAAGGCACATC > NC_000913/3783308‑3783496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |