Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,828,936 T→C intergenic (‑271/‑9) gltS ← / → xanP glutamate transporter/xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,828,9360TC93.1% 175.6 / 2.4 58intergenic (‑271/‑9)gltS/xanPglutamate transporter/xanthine permease
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (29/25);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCG  >  NC_000913/3828842‑3829030
                                                                                              |                                                                                              
tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGttt                                                                                           <  2:79122/100‑1 (MQ=255)
  cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAACTGCCCGCTTTGCTACCACGGGATTGTTTTc                                                                                         >  1:902845/1‑100 (MQ=255)
  cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTc                                                                                         <  2:535116/100‑1 (MQ=255)
  cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTc                                                                                         <  2:317048/100‑1 (MQ=255)
     atctctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGAt                                                                                      <  2:1205132/97‑1 (MQ=255)
      tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATg                                                                                     >  2:200395/1‑100 (MQ=255)
         ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGAtagat                                                                                  >  1:1205143/1‑96 (MQ=255)
         ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCt                                                                                  <  1:1098798/100‑1 (MQ=255)
           cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGt                                                                                >  2:836430/1‑100 (MQ=255)
             tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGTGACTGTTTTCGATGTCTGttt                                                                              >  2:711696/1‑100 (MQ=255)
             tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGttt                                                                              <  1:1187070/100‑1 (MQ=255)
             tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGttt                                                                              <  1:1187825/100‑1 (MQ=255)
                 aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCAc                                                                          <  1:1190079/100‑1 (MQ=255)
                        aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAg                                                                   >  1:1039560/1‑100 (MQ=255)
                         gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTCTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGt                                                                  >  1:853586/1‑100 (MQ=255)
                           tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCa                                                                >  1:605803/1‑100 (MQ=255)
                           tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCACGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCCCGAGTCa                                                                >  2:1213694/1‑100 (MQ=255)
                            gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAg                                                               >  2:773878/1‑100 (MQ=255)
                                 tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAt                                                          <  2:380372/100‑1 (MQ=255)
                                  gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATg                                                         <  2:2873/100‑1 (MQ=255)
                                    ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcg                                                       >  2:1178303/1‑100 (MQ=255)
                                    ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcg                                                       >  2:633885/1‑100 (MQ=255)
                                     gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcg                                                       <  1:711701/99‑1 (MQ=255)
                                      cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcgc                                                      >  2:727340/1‑99 (MQ=255)
                                      cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATgcgc                                                      <  2:248632/99‑1 (MQ=255)
                                      cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACAGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCa                                                     >  2:852249/1‑100 (MQ=255)
                                          cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCCCGAGTCAGAAAATGCGCAACCg                                                 >  2:1191492/1‑100 (MQ=255)
                                          cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCCCGAGTCAGAAAATGCGCAACCg                                                 >  2:1191607/1‑100 (MQ=255)
                                               gCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                            <  1:995331/100‑1 (MQ=255)
                                               gCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                            >  1:1233976/1‑100 (MQ=255)
                                                cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                           >  2:125775/1‑100 (MQ=255)
                                                  tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCa                                         <  1:621604/100‑1 (MQ=255)
                                                    tctcCTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                       >  2:642683/1‑100 (MQ=255)
                                                     ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                      <  2:44146/100‑1 (MQ=255)
                                                     ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                      <  1:1077370/100‑1 (MQ=255)
                                                      tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                     <  2:1003270/100‑1 (MQ=255)
                                                       ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTc                                    <  2:256650/100‑1 (MQ=255)
                                                        tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                   >  2:909026/1‑100 (MQ=255)
                                                        tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                   >  2:500584/1‑100 (MQ=255)
                                                        tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCCCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                   <  1:1191503/100‑1 (MQ=255)
                                                        tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCCCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                   <  1:1191618/100‑1 (MQ=255)
                                                          tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaa                                 >  2:18455/1‑100 (MQ=255)
                                                            ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                               <  2:902838/100‑1 (MQ=255)
                                                               cAGCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                            >  2:851789/1‑100 (MQ=255)
                                                                 gCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                          >  2:17872/1‑100 (MQ=255)
                                                                 gCTAAAATGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                          >  1:89788/1‑100 (MQ=255)
                                                                     aaaTGCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAACAAAGCGAACTg                      >  2:929219/1‑100 (MQ=255)
                                                                         gCCCGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                  <  1:610711/100‑1 (MQ=255)
                                                                          cccGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTa                 >  1:310180/1‑100 (MQ=255)
                                                                           ccGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                >  1:213656/1‑100 (MQ=255)
                                                                           ccGCTTTGCTACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                <  1:200397/100‑1 (MQ=255)
                                                                                  gCTAGCACGGGACTGTTGTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt           <  2:287373/98‑1 (MQ=255)
                                                                                    tACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa       >  1:253029/1‑100 (MQ=255)
                                                                                    tACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa       <  1:263678/100‑1 (MQ=255)
                                                                                    tACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa       >  2:531529/1‑100 (MQ=255)
                                                                                    tACCACGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa        >  2:515684/1‑99 (MQ=255)
                                                                                     aCCACGGGACGGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGTCTTAAAACAGCGAACTGATTTACCGTCTTGAAg      <  2:856154/100‑1 (MQ=255)
                                                                                         cGGGACTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg  <  2:350742/100‑1 (MQ=255)
                                                                                              |                                                                                              
TGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCG  >  NC_000913/3828842‑3829030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: