| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,853,909 | A→G | intergenic (+267/‑13) | tisB → / → emrD | toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,853,909 | 0 | A | G | 98.0% | 160.5 / ‑4.2 | 49 | intergenic (+267/‑13) | tisB/emrD | toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (27/21); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
CTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT > NC_000913/3853815‑3854004 | cTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCAt > 1:190995/1‑99 (MQ=255) tGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATcc < 2:495308/100‑1 (MQ=255) atatCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATa < 1:621822/100‑1 (MQ=255) tatCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATaa > 2:436454/1‑100 (MQ=255) tatCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATa > 2:755439/1‑99 (MQ=255) atCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAAt < 2:458302/100‑1 (MQ=255) cTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCCTTTATGGGAATGCGTAGTGACATATTCTAATTATTTTTATAAAGGCATCCGTGATAATg > 2:571637/1‑99 (MQ=255) tGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaa > 2:311269/1‑100 (MQ=255) ggCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaaa > 1:674522/1‑100 (MQ=255) cTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAg > 2:1296084/1‑100 (MQ=255) aaCATTCATCAATGTGATAGATTCCTCTCCCGCGTTTATGGGATTGCGTAGTGACTTATTCTAATTATTTTTATATAGGCATCCGTGATAATGAAAAGGc > 2:1215764/1‑100 (MQ=255) catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaa > 1:1058973/1‑100 (MQ=255) catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaa > 1:677255/1‑100 (MQ=255) tcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTCGTGACTTATTCTAATTATTTTTATAAAGGCATCCCTCATAATGAAAAGGCACAGAAAc > 2:1126470/1‑100 (MQ=255) caATGTGATAGATTCCTCTCCCGCATTTATGGTAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg < 2:150010/100‑1 (MQ=255) caATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg < 1:875911/100‑1 (MQ=255) aaTGTGATAGATTCCTCTCCCGCATTTATGGTAATGCGCAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg < 2:915073/99‑1 (MQ=255) aaTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGt > 2:1016542/1‑100 (MQ=255) tAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTg < 2:190993/100‑1 (MQ=255) tAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTg < 2:1167958/100‑1 (MQ=255) ttCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAt < 2:754/100‑1 (MQ=255) cccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGtt > 1:228157/1‑100 (MQ=255) cccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGtt > 1:829904/1‑100 (MQ=255) ccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTg < 2:12837/100‑1 (MQ=255) ccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTg < 2:134732/100‑1 (MQ=255) cATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGt < 2:16629/99‑1 (MQ=255) aTTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTAt > 1:818869/1‑100 (MQ=255) tATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGTCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTAc > 2:157601/1‑100 (MQ=255) aTGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACt < 2:1065918/100‑1 (MQ=255) tGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTc > 1:1268389/1‑100 (MQ=255) aTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc < 1:1063280/100‑1 (MQ=255) aTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc < 1:188976/100‑1 (MQ=255) gCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCg > 2:921666/1‑100 (MQ=255) gCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCg > 2:415060/1‑100 (MQ=255) aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGTTCGTCgg > 1:400312/1‑100 (MQ=255) aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCgg > 2:593554/1‑100 (MQ=255) gTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGt < 1:1296095/100‑1 (MQ=255) aCTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCa > 1:865054/1‑99 (MQ=255) cTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGa > 1:186230/1‑100 (MQ=255) aTTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTGTTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATgg > 2:769402/1‑100 (MQ=255) ttCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgc < 2:309699/100‑1 (MQ=255) tCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcg < 2:955179/100‑1 (MQ=255) cTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgc < 2:1058963/100‑1 (MQ=255) aaTTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCaa > 1:1100215/1‑100 (MQ=255) aaTTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTAGTGGCCGTCGGTCAGATAGCGCaa > 2:299148/1‑100 (MQ=255) ttattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAAc > 2:190714/1‑100 (MQ=255) tattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAAcc < 1:1266943/100‑1 (MQ=255) ttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAtt < 1:1022036/100‑1 (MQ=255) taAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTatat > 2:108722/1‑100 (MQ=255) | CTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT > NC_000913/3853815‑3854004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |