Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,281,967 T→C Q370R (CAG→CGG)  yjcF ← pentapeptide repeats protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,281,9670TC96.0% 244.4 / ‑0.9 75Q370R (CAG→CGG) yjcFpentapeptide repeats protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (37/34);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTA  >  NC_000913/4281871‑4282060
                                                                                                |                                                                                             
gCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCgg                                                                                             <  2:259696/99‑1 (MQ=255)
 cacaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGAc                                                                                           <  1:348186/100‑1 (MQ=255)
  acaATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACg                                                                                          >  1:1051675/1‑100 (MQ=255)
      tcttctTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAg                                                                                      >  2:336489/1‑100 (MQ=255)
          cttcAAAGNATTTCTGTAGTGTTACCNGGATNTCNCTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCNAGCCTc                                                                                  >  1:1202333/1‑100 (MQ=255)
           ttcAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCa                                                                                 >  1:1226967/1‑100 (MQ=255)
            tcAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCAt                                                                                >  1:999912/1‑100 (MQ=255)
             cAAAGAATTTCTGTAGTGTTACCAGGCTATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATc                                                                               >  1:793983/1‑100 (MQ=255)
             cAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATc                                                                               <  1:926815/100‑1 (MQ=255)
             cAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATc                                                                               <  1:363142/100‑1 (MQ=255)
              aaaGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCg                                                                              <  2:1057167/100‑1 (MQ=255)
              aaaGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCg                                                                              <  2:693914/100‑1 (MQ=255)
              aaaGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCg                                                                              <  2:544375/100‑1 (MQ=255)
              aaaGAATTTCTGTAGTGTTACCAGGATATCACTATGAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCAATTCCCTGGACGCTAGCCTCATCg                                                                              >  2:1038734/1‑100 (MQ=255)
               aaGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTTCCCGGACGCTAGCCTCATCGa                                                                             >  2:813703/1‑100 (MQ=255)
                 gAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGaaa                                                                           >  1:1099364/1‑100 (MQ=255)
                   aTTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAAtt                                                                         <  1:756105/100‑1 (MQ=255)
                       cTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAaca                                                                     <  1:758856/100‑1 (MQ=255)
                        tGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAacac                                                                    <  1:1031074/100‑1 (MQ=255)
                         gTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACg                                                                   <  1:582594/100‑1 (MQ=255)
                            gtgtTACCAGGATATCACTATTCATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCTCCGGACGCTAGCCTCATCGAAATTAACACGATa                                                                >  2:758103/1‑100 (MQ=255)
                            gtgtTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATa                                                                >  2:754964/1‑100 (MQ=255)
                             tgtTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATag                                                               >  1:20262/1‑100 (MQ=255)
                                  ccAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACg                                                          >  2:694327/1‑100 (MQ=255)
                                   cAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACgg                                                         >  1:534432/1‑100 (MQ=255)
                                      gATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGata                                                      <  1:689283/100‑1 (MQ=255)
                                      gATATCACTAGTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGata                                                      >  2:179281/1‑100 (MQ=255)
                                         atCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTa                                                   <  2:1116569/100‑1 (MQ=255)
                                          tCACTATTAATGGAGATATTTTCCTCACCACGTAGAATGCTACTACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTaa                                                  <  2:915357/100‑1 (MQ=255)
                                          tCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTaa                                                  <  1:519613/100‑1 (MQ=255)
                                           cACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTaaa                                                 <  2:318075/100‑1 (MQ=255)
                                            aCTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAAc                                                <  1:1229284/100‑1 (MQ=255)
                                             cTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACa                                               <  1:525308/100‑1 (MQ=255)
                                                  aaTGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAg                                          >  1:899306/1‑100 (MQ=255)
                                                  aaTGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAg                                          <  2:211421/100‑1 (MQ=255)
                                                  aaTGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATAAAACATca                                            >  2:1005358/1‑97 (MQ=255)
                                                    tGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGc                                        <  1:393162/100‑1 (MQ=255)
                                                     ggAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCt                                       >  2:921069/1‑100 (MQ=255)
                                                     ggAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCt                                       >  2:1160834/1‑100 (MQ=255)
                                                        gaTATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCtttt                                    >  2:168096/1‑100 (MQ=255)
                                                          tatTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGt                                  <  2:899299/100‑1 (MQ=255)
                                                          tatTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGt                                  >  2:986282/1‑100 (MQ=255)
                                                          tatTTTCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGt                                  <  1:72360/100‑1 (MQ=255)
                                                             tttCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTaa                               <  2:1211674/100‑1 (MQ=255)
                                                             tttCCTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTaa                               >  1:433673/1‑100 (MQ=255)
                                                                ccTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaac                            <  2:160864/100‑1 (MQ=255)
                                                                ccTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaac                            <  1:1205339/100‑1 (MQ=255)
                                                                ccTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaac                            <  2:534428/100‑1 (MQ=255)
                                                                 cTCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaaca                           >  2:745618/1‑100 (MQ=255)
                                                                  tCACCACGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacag                          >  1:1134325/1‑100 (MQ=255)
                                                                   caccacGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacaga                         <  2:1230788/100‑1 (MQ=255)
                                                                   caccacGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacaga                         >  1:371840/1‑100 (MQ=255)
                                                                    accacGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacagaa                        >  2:1065932/1‑100 (MQ=255)
                                                                     ccacGGAGAATGGTAATACCCATTTCAAGGACGCTGGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacag                          <  2:463105/97‑1 (MQ=255)
                                                                      cacGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacagaaca                      >  2:253912/1‑100 (MQ=255)
                                                                       acGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAaacagaacag                     >  2:375114/1‑100 (MQ=255)
                                                                        cGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGt                    >  2:704979/1‑100 (MQ=255)
                                                                        cGGAGAATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCATTTGTTAAaacagaacag                     >  2:335730/1‑99 (MQ=255)
                                                                         ggAGAATGGTAATACCCATTCCCCGGACGCTAGCCTAATCGAAATTAACACGATAGAGACGGATATTAAACATCACGGCTTTTGTTAAAAAAGAACAGtt                   >  2:645700/1‑100 (MQ=255)
                                                                          gagaATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTg                  <  2:927788/100‑1 (MQ=255)
                                                                            gaATGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGaa                >  1:1304888/1‑100 (MQ=255)
                                                                              ttccgatctcccATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAAt              <  1:947128/91‑1 (MQ=255)
                                                                              aTGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAAt              <  2:1068279/100‑1 (MQ=255)
                                                                               tGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATg             >  1:801164/1‑100 (MQ=255)
                                                                               tGGTAATACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATg             <  2:9738/100‑1 (MQ=255)
                                                                                   aaTACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCaa         >  1:293666/1‑100 (MQ=255)
                                                                                     tACCCATTCCGCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAAtt       <  2:999903/100‑1 (MQ=255)
                                                                                     tACCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAAtt       >  1:694697/1‑100 (MQ=255)
                                                                                      aCCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTg      >  2:878056/1‑100 (MQ=255)
                                                                                      aCCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTg      <  1:1249974/100‑1 (MQ=255)
                                                                                      aCCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTg      >  2:991849/1‑100 (MQ=255)
                                                                                      aCCCATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTg      <  1:254232/100‑1 (MQ=255)
                                                                                       cccATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAAtagatcggaa     >  2:947120/1‑91 (MQ=255)
                                                                                       cccATTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGc     >  1:993139/1‑100 (MQ=255)
                                                                                          aTTCCCCGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTa  <  1:421615/100‑1 (MQ=255)
                                                                                                |                                                                                             
GCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTA  >  NC_000913/4281871‑4282060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑