Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,479,551 A→G intergenic (‑14/‑179) yjgM ← / → yjgN GNAT family putative N‑acetyltransferase/DUF898 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,479,5510AG87.9% 159.4 / 7.0 58intergenic (‑14/‑179)yjgM/yjgNGNAT family putative N‑acetyltransferase/DUF898 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base G (26/25);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGACGAATGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGA  >  NC_000913/4479453‑4479646
                                                                                                  |                                                                                               
ccTGACGAATGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa                                                                                                >  1:571679/1‑100 (MQ=255)
 cTGACGAATGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaatgaa                                                                                               <  2:819547/100‑1 (MQ=255)
  tGACGAATGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaatgaaa                                                                                              >  2:1292047/1‑100 (MQ=255)
      gAATGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaatgaaaataa                                                                                          <  2:367904/100‑1 (MQ=255)
        aTGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAg                                                                                        >  1:1136409/1‑100 (MQ=255)
        aTGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                                                                        <  1:197092/100‑1 (MQ=255)
            cgcgGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAAt                                                                                    >  2:205037/1‑100 (MQ=255)
               gggCGATAGCAGGATTATCGTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATta                                                                                  <  2:348022/99‑1 (MQ=255)
                ggCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCAGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATtata                                                                                <  2:608382/100‑1 (MQ=255)
                    aTAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACAtt                                                                            <  1:544444/100‑1 (MQ=255)
                     naGCAGGATTATCCTGCAGCATCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACAttt                                                                           >  2:795704/2‑100 (MQ=255)
                       gCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGt                                                                         >  1:1055316/1‑100 (MQ=255)
                        cAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGtt                                                                        >  1:273145/1‑100 (MQ=255)
                        cAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGtt                                                                        >  2:1199977/1‑100 (MQ=255)
                        cAGGAGTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGtt                                                                        <  1:759569/100‑1 (MQ=255)
                         aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGttt                                                                       <  1:1160159/100‑1 (MQ=255)
                         aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGttt                                                                       <  1:1076731/100‑1 (MQ=255)
                               aTCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATAcc                                                                 <  2:460649/100‑1 (MQ=255)
                                 ccTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCAc                                                               <  1:346845/100‑1 (MQ=255)
                                 ccTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCAc                                                               >  1:175479/1‑100 (MQ=255)
                                   tGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCActc                                                             <  1:662021/100‑1 (MQ=255)
                                     cAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCActctc                                                           >  1:812309/1‑100 (MQ=255)
                                      aGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctct                                                          >  1:930391/1‑100 (MQ=255)
                                      aGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctct                                                          <  1:739892/100‑1 (MQ=255)
                                       gCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCtctt                                                         >  1:13174/1‑100 (MQ=255)
                                        cGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCtcttc                                                        <  1:745195/100‑1 (MQ=255)
                                         gTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCtcttct                                                       >  1:647042/1‑100 (MQ=255)
                                         gTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCtcttct                                                       >  1:189712/1‑100 (MQ=255)
                                          tCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTcttctt                                                      <  2:473710/100‑1 (MQ=255)
                                           cAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTta                                                     <  2:998130/100‑1 (MQ=255)
                                           cAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTta                                                     >  2:496929/1‑100 (MQ=255)
                                            aGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTtac                                                    >  1:178522/1‑100 (MQ=255)
                                               cGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTtacgta                                                 >  1:464293/1‑100 (MQ=255)
                                                    cATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCaa                                            >  1:383645/1‑100 (MQ=255)
                                                      ttACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGc                                          >  1:640954/1‑100 (MQ=255)
                                                       tACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGcn                                         <  1:795709/100‑2 (MQ=255)
                                                         cAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGAc                                       >  2:828601/1‑100 (MQ=255)
                                                            gTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTta                                    <  1:176298/100‑1 (MQ=255)
                                                             tGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTtat                                   >  2:1208512/1‑100 (MQ=255)
                                                                  gcggcgCAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGt                              <  1:630525/100‑1 (MQ=255)
                                                                   cggcgCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGta                             >  2:813995/1‑100 (MQ=255)
                                                                       gcAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACTCAAGCGACTTATAAAGtaataa                         <  2:759086/100‑1 (MQ=255)
                                                                       gcAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGtaataa                         >  1:629904/1‑100 (MQ=255)
                                                                       gcAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGtaataa                         <  1:96733/100‑1 (MQ=255)
                                                                       gcAATGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGtaataa                         >  1:101485/1‑100 (MQ=255)
                                                                         aaTGTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTAGACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAg                       >  2:714560/1‑100 (MQ=255)
                                                                         aaTGTTATTCATAGTTTAACCAAATAAAAATAAAGTAATTATACATTTCTTTAATACCACTCTCCTCTTACGTACGCAAGCGACTTATAAAGt                              <  2:188787/93‑1 (MQ=255)
                                                                            gTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGttt                    <  2:869381/100‑1 (MQ=255)
                                                                            gTTATTCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGttt                    <  2:1158027/100‑1 (MQ=255)
                                                                                 tCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGcc               <  2:129701/100‑1 (MQ=255)
                                                                                 tCATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGcc               <  2:998291/100‑1 (MQ=255)
                                                                                  cATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCa              >  1:819521/1‑100 (MQ=255)
                                                                                  cATAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCa              >  1:618268/1‑100 (MQ=255)
                                                                                   aTAGTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAg             >  2:783766/1‑100 (MQ=255)
                                                                                   aTAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCGTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGcc               <  2:519141/98‑1 (MQ=255)
                                                                                      gTTTAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCt          >  1:799450/1‑100 (MQ=255)
                                                                                         tAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTAtt       <  2:1090287/100‑1 (MQ=255)
                                                                                         tAACCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTAtt       <  1:783771/100‑1 (MQ=255)
                                                                                           aCCCAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTAc     >  2:532269/1‑100 (MQ=255)
                                                                                            cccAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACt    <  2:501212/100‑1 (MQ=255)
                                                                                             ccAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGGGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACt    <  2:802419/99‑1 (MQ=255)
                                                                                              cAATGAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGa  >  2:1126367/1‑100 (MQ=255)
                                                                                                  |                                                                                               
CCTGACGAATGACGCGGGCGATAGCAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGTACGCAAGCGACTTATAAAGTAATAAAGTTTTTGCCAGCCTATTACTGA  >  NC_000913/4479453‑4479646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: