Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,490,055 | T→G | E3D (GAA→GAC) | yjgR ← | DUF853 family protein with NTPase fold |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,490,055 | 0 | T | G | 98.0% | 172.9 / ‑3.4 | 49 | E3D (GAA→GAC) | yjgR | DUF853 family protein with NTPase fold |
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (24/24); total (25/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 |
TACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489958‑4490150 | tACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTc < 2:765724/100‑1 (MQ=255) ccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACt > 1:1080208/1‑100 (MQ=255) ccGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACt < 1:832861/100‑1 (MQ=255) gTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCa > 1:67460/1‑100 (MQ=255) ccccGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATg < 2:970774/100‑1 (MQ=255) cGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATgaga < 1:773399/100‑1 (MQ=255) gcgcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtc > 1:823249/1‑100 (MQ=255) cgcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcc < 1:280454/100‑1 (MQ=255) gcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcct > 2:1031452/1‑100 (MQ=255) gcCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcct > 1:801126/1‑100 (MQ=255) cGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcctcc < 1:925268/100‑1 (MQ=255) cGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATAtcctcc < 2:1041931/100‑1 (MQ=255) cAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc < 1:434147/100‑1 (MQ=255) cAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc > 1:491915/1‑100 (MQ=255) cAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcc > 2:763011/1‑100 (MQ=255) gCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctg > 2:75968/1‑100 (MQ=255) cccATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTcctgc > 1:146015/1‑100 (MQ=255) tGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTgg < 2:958028/100‑1 (MQ=255) gTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtat > 1:338953/1‑100 (MQ=255) gTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGtat < 2:702842/100‑1 (MQ=255) tAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAg < 1:1192081/100‑1 (MQ=255) aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg < 2:338950/100‑1 (MQ=255) aGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAgg > 2:736211/1‑100 (MQ=255) gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc > 2:633213/1‑100 (MQ=255) gCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGc < 1:6786/100‑1 (MQ=255) cATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCaa > 1:871855/1‑100 (MQ=255) aTTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAAc < 1:895921/100‑1 (MQ=255) ttCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCAGTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACg > 2:831868/1‑100 (MQ=255) cccGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTc > 1:51030/1‑100 (MQ=255) gCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGc < 1:1031461/100‑1 (MQ=255) aaaaCAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAagag < 1:867277/100‑1 (MQ=255) cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa < 2:910179/100‑1 (MQ=255) cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa < 2:910271/100‑1 (MQ=255) cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa > 1:383213/1‑100 (MQ=255) cAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATa < 2:521957/100‑1 (MQ=255) tCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACgtg > 1:488560/1‑100 (MQ=255) ccGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGaa > 2:615237/1‑100 (MQ=255) cGGCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGAATAGGCAACGTCCGGCAAAGAGAACACGTGTAATGAag < 2:804884/100‑1 (MQ=255) gCGTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAagag < 2:491911/100‑1 (MQ=255) gCGTGCGGGCAATTAACAGGGGGTCACTCAAGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTATAATGAagag > 2:855704/1‑100 (MQ=255) gTGCGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGaa > 2:314701/1‑100 (MQ=255) gCGGGCAATTAACAGGGGGTCACTCATGAGATCTGCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGCATACGTGTAGTGAAGAGaaga > 1:608780/1‑98 (MQ=255) cGGGCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAAt > 1:740403/1‑100 (MQ=255) gCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAc < 1:896222/100‑1 (MQ=255) gCAATTAACAGGGGGTCACTCATGAGATATCCTCCAGGTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAc < 2:1228708/100‑1 (MQ=255) cAATTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAAcc > 1:98182/1‑100 (MQ=255) aaTTAACAGGGCGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCt > 2:809007/1‑100 (MQ=255) aTTAACAGGGGGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTa < 2:823243/100‑1 (MQ=255) ggggGTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCgtg > 2:806250/1‑100 (MQ=255) | TACCCGTCCCCGTTGCGCCGGTAATCAGCCCATGACGGTTAGCCATTCCCGGCAGTAAAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTG > NC_000913/4489958‑4490150 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |