| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 426,040 | A→T | T344S (ACG→TCG) | queA → | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 426,040 | 0 | A | T | 97.2% | 118.0 / ‑3.5 | 36 | T344S (ACG→TCG) | queA | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (16/19); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
TCGGCCTTTGCCGGTTATCAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCACGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAG > NC_000913/425944‑426131 | tCGGCCTTTGCCGGTTATCAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGt > 1:1152304/1‑100 (MQ=255) cGGCCTTTGCCGGTTATCAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTa > 1:397434/1‑100 (MQ=255) tGCCGGTTATCAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCg < 2:1190821/100‑1 (MQ=255) ggTTATCAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAgg < 2:952756/100‑1 (MQ=255) ttATCAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCa > 2:93998/1‑100 (MQ=255) cAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTa < 2:656679/100‑1 (MQ=255) cAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTa > 2:930502/1‑100 (MQ=255) cAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTa > 2:929019/1‑100 (MQ=255) cAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGCAATGCGATGTTTATCTCGTACAATCCGCAGGCAATTa > 1:551509/1‑100 (MQ=255) aaCACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTaa > 2:723143/1‑100 (MQ=255) acacCATGAGCGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGa < 2:744463/100‑1 (MQ=255) cATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTTCGTTGATGCGATTTTTATCTTGTACAATCCGCAGTAAATTAATGa > 2:682272/1‑96 (MQ=255) aTGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGcgcg < 1:668864/100‑1 (MQ=255) aTGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGcgcg < 1:778871/100‑1 (MQ=255) gCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCgggg < 1:1000267/100‑1 (MQ=255) tataAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGt > 2:330945/1‑100 (MQ=255) cagcagTAGAAGAGAAATATCGATTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCcg < 2:240588/100‑1 (MQ=255) ggTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCgcggc < 1:753802/100‑1 (MQ=255) ggTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCgcggc > 2:1085071/1‑100 (MQ=255) aagaGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTgg > 2:75749/1‑100 (MQ=255) agagAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGt > 2:1286910/1‑100 (MQ=255) agagAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGt > 1:586182/1‑100 (MQ=255) agaAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGttt < 2:420056/100‑1 (MQ=255) gaAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTa > 2:112340/1‑100 (MQ=255) aaTATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTaaa < 1:723148/100‑1 (MQ=255) cGCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGtt < 1:404650/100‑1 (MQ=255) gCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTg < 2:579412/100‑1 (MQ=255) gCTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTg < 1:94000/100‑1 (MQ=255) gCTTTTTTAGTTACGGTGATGCGATGTTTATCACGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTg > 2:276969/1‑100 (MQ=255) cTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTgg < 1:776919/100‑1 (MQ=255) cTTTTTTAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTgg > 1:323202/1‑100 (MQ=255) tttttAGTTACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGAc < 1:118647/100‑1 (MQ=255) tACTGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTc < 1:534589/100‑1 (MQ=255) tACGGTGATGCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTc < 1:1260388/100‑1 (MQ=255) gCGATGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAg < 1:220100/99‑1 (MQ=255) tGTTTATCTCGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGgag > 2:168652/1‑100 (MQ=255) | TCGGCCTTTGCCGGTTATCAACACACCATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCACGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAG > NC_000913/425944‑426131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |