Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,615,859 | T→G | T403P (ACC→CCC) | yjjI ← | DUF3029 family protein, putative glycine radical enzyme |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,615,859 | 0 | T | G | 96.2% | 172.3 / NA | 54 | T403P (ACC→CCC) | yjjI | DUF3029 family protein, putative glycine radical enzyme |
Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (26/25); total (28/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTT > NC_000913/4615763‑4615955 | ccTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGAt > 1:677093/1‑100 (MQ=255) ccTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGAt > 2:466881/1‑100 (MQ=255) cTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCGGGAATAATAATAAGCATGATGCGGGGCGACAGTTGGCAGATGGGTGATc > 2:390580/1‑100 (MQ=255) tGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCg < 1:700570/100‑1 (MQ=255) cGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGa < 1:994516/100‑1 (MQ=255) gTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCt < 1:1093445/100‑1 (MQ=255) gTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCt < 1:404427/100‑1 (MQ=255) aCGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc < 1:173228/100‑1 (MQ=255) aCGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc < 1:1069105/100‑1 (MQ=255) aCGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc > 1:1252123/1‑100 (MQ=255) aCGTGTGGTGGTGTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc < 1:892197/100‑1 (MQ=255) cGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc > 1:392012/1‑99 (MQ=255) gTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTAATGCCGGAATAATCATAAGCATGATGCGGGGCGGCAGTGTGCGGATGGGGGATCGGATCTGGCTc > 2:1105243/1‑100 (MQ=255) tGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATc > 1:902793/1‑100 (MQ=255) gATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCg < 2:404474/100‑1 (MQ=255) gTTTGGTCGAGCGTCAGAATGTGGCTGATGCCGGTATAATAATAAGCATTAGGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCa < 2:1150396/99‑1 (MQ=255) gTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCAt > 1:211449/1‑100 (MQ=255) gTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCAt < 2:612646/100‑1 (MQ=255) gtcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGc > 1:507763/1‑100 (MQ=255) tcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCa < 2:614932/100‑1 (MQ=255) cgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCaa > 1:172706/1‑100 (MQ=255) cGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAcgc > 2:238599/1‑100 (MQ=255) cAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAcgcgcg > 2:440594/1‑100 (MQ=255) aGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACgcgcgc < 1:209462/100‑1 (MQ=255) cGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCgt > 2:924561/1‑100 (MQ=255) gCTGATGCCTGAATAATAATAAGCATGATTCGGGGCGACAGTTTGCAGATGGGGGATCTGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCgtg < 2:469711/100‑1 (MQ=255) cTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCgtgg > 1:749062/1‑100 (MQ=255) tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc < 1:185211/100‑1 (MQ=255) tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc > 2:759360/1‑100 (MQ=255) tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc < 2:390314/100‑1 (MQ=255) tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc > 2:349008/1‑100 (MQ=255) gCCGGAATAATAATAAGCATGATGCGGGGCGACAGCCTGCAGATGGGAGAACGGATCGGCCTCATCGCCATACTCCAAACTCGCGCCCGACg > 2:912791/1‑92 (MQ=255) ccGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGa > 2:1004084/1‑100 (MQ=255) ccGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGa > 1:725604/1‑100 (MQ=255) ggAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGata > 2:995428/1‑100 (MQ=255) ataataataAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCg > 2:379903/1‑100 (MQ=255) ataataataAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCg < 2:392008/100‑1 (MQ=255) taataataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCgg < 2:899093/100‑1 (MQ=255) taataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAAc < 1:440598/100‑1 (MQ=255) gCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcc > 1:1226434/1‑100 (MQ=255) cATGATGCGGGGCGACAGTTTGCAGATGGTGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcc < 2:311295/99‑1 (MQ=255) atgatgCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcccc < 2:162243/100‑1 (MQ=255) atgatgCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcccc > 1:953189/1‑100 (MQ=255) atgatgCGGGGCGACAGTTTGCAGATGGAGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcccc > 1:952975/1‑100 (MQ=255) gCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtg < 1:238601/100‑1 (MQ=255) gggCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGc > 2:408111/1‑100 (MQ=255) gACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgtgt < 1:851793/100‑1 (MQ=255) gACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgtgt < 2:677089/100‑1 (MQ=255) gACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgtgt < 1:163815/100‑1 (MQ=255) cAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTaa > 2:284996/1‑100 (MQ=255) cAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTaa < 2:172704/100‑1 (MQ=255) cAGTTTGCAGATGGGGGATCGGATCTAGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTa > 2:604615/1‑99 (MQ=255) ttGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATg < 2:1026139/100‑1 (MQ=255) cAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGgcg > 1:439216/1‑100 (MQ=255) ggggGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGttt > 1:1153311/1‑100 (MQ=255) | CCTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTT > NC_000913/4615763‑4615955 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |