Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 106,554 A→G intergenic (+98/‑3) ftsZ → / → lpxC GTP‑binding tubulin‑like cell division protein/UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913106,5540AG94.8% 186.9 / ‑1.8 58intergenic (+98/‑3)ftsZ/lpxCGTP‑binding tubulin‑like cell division protein/UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (21/34);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTGACTGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATACGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCG  >  NC_000913/106458‑106649
                                                                                                |                                                                                               
aaTTGACTGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCga                                                                                              >  1:326312/1‑100 (MQ=255)
 aTTGACTGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCgat                                                                                             <  1:800070/100‑1 (MQ=255)
    gACTGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCgatgat                                                                                          >  2:535940/1‑100 (MQ=255)
     aCTGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATc                                                                                         <  1:699620/100‑1 (MQ=255)
      cTGGAATTTGGGTTTCGCGACTCTTTGTGCTAAACCGGCCTGCAAAATGTATAGTACACTTCGGCTGGAGAGGTCACTTGGCGAGATGATACgaaga                                                                                           >  2:583993/1‑97 (MQ=255)
       tGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATcaa                                                                                       <  1:220666/100‑1 (MQ=255)
        ggAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATcaaa                                                                                      >  2:382843/1‑100 (MQ=255)
          aaTTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATcaaaca                                                                                    <  2:402212/100‑1 (MQ=255)
           aTTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATcaaacaa                                                                                   <  2:967850/100‑1 (MQ=255)
           aTTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATcaaacaa                                                                                   >  1:553864/1‑100 (MQ=255)
           aTTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGACGCATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATccaa                                                                                      <  2:1170969/97‑1 (MQ=255)
                 gTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGaca                                                                             >  2:636791/1‑100 (MQ=255)
                 gTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGaca                                                                             <  2:283383/100‑1 (MQ=255)
                   ttCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATACGATGATCAAACAAAGGACACt                                                                           <  2:853377/100‑1 (MQ=255)
                        ggCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAAc                                                                      >  1:339290/1‑100 (MQ=255)
                            ctTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTAt                                                                  <  1:1147064/100‑1 (MQ=255)
                               tgtgCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGt                                                               <  1:977662/100‑1 (MQ=255)
                                 tgCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTc                                                             >  2:1215512/1‑100 (MQ=255)
                                   cTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTATACGTATCGTTctg                                                           <  1:879432/100‑3 (MQ=255)
                                    tAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAgg                                                          >  2:198307/1‑100 (MQ=255)
                                     aaaCTGGCCCGCCTAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGc                                                         <  2:136685/100‑1 (MQ=255)
                                     aaaCTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGc                                                         >  1:886601/1‑100 (MQ=255)
                                           gCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACggg                                                   <  2:326309/100‑1 (MQ=255)
                                                 cgAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCgg                                             >  2:1286560/1‑100 (MQ=255)
                                                    aTGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGttt                                          <  2:394026/100‑1 (MQ=255)
                                                    aTGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGttt                                          <  2:593840/100‑1 (MQ=255)
                                                    aTGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGttt                                          <  2:624087/100‑1 (MQ=255)
                                                     tGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTa                                         <  2:166502/100‑1 (MQ=255)
                                                     tGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTa                                         <  2:1130344/100‑1 (MQ=255)
                                                     tGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTa                                         <  1:116029/100‑1 (MQ=255)
                                                      gTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTAc                                        <  1:1247100/100‑1 (MQ=255)
                                                      gTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTAc                                        <  1:35386/100‑1 (MQ=255)
                                                       tataGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACa                                       >  1:597267/1‑100 (MQ=255)
                                                           gTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATAcc                                   >  2:796842/1‑100 (MQ=255)
                                                             acacTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCgg                                 <  2:1290159/100‑1 (MQ=255)
                                                               acTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCa                               <  1:648229/100‑1 (MQ=255)
                                                                cTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCgg                                 >  2:222355/1‑97 (MQ=255)
                                                                cTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCaa                              >  1:1168429/1‑100 (MQ=255)
                                                                cTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCaa                              >  1:1148683/1‑100 (MQ=255)
                                                                cTTCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCaa                              <  2:228894/100‑1 (MQ=255)
                                                                 ttCGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAg                             <  1:1196646/100‑1 (MQ=255)
                                                                  tCGGTTGGATAGGTAATTTGGCGAGATAATACGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGa                            <  2:883154/100‑1 (MQ=255)
                                                                   cGGTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGaa                           <  1:469090/100‑1 (MQ=255)
                                                                     gTTGGATAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAg                         <  2:1016687/100‑1 (MQ=255)
                                                                          aTAGGTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCAcc                    <  1:535944/100‑1 (MQ=255)
                                                                             ggTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTCAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTg                 <  2:835022/100‑1 (MQ=255)
                                                                             ggTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTg                 >  2:1162149/1‑100 (MQ=255)
                                                                             ggTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTg                 <  1:200725/100‑1 (MQ=255)
                                                                             ggTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTg                 <  2:880415/100‑1 (MQ=255)
                                                                             ggTAATTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTg                 >  1:1049460/1‑100 (MQ=255)
                                                                                aaTTTGGCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACg              >  1:151831/1‑100 (MQ=255)
                                                                                      gCGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGc        <  2:163352/100‑1 (MQ=255)
                                                                                       cGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGcc       <  1:174115/100‑1 (MQ=255)
                                                                                       cGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGcc       <  1:198309/100‑1 (MQ=255)
                                                                                       cGAGATAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATAACGGCAAGAAAGTCACCCTGACGTTACGcc       >  2:508981/1‑100 (MQ=255)
                                                                                        gagaTAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGccc      <  2:493410/100‑1 (MQ=255)
                                                                                         agaTAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCt     >  2:75669/1‑100 (MQ=255)
                                                                                          gaTAATGCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTg    >  2:997405/1‑100 (MQ=255)
                                                                                            taatgCGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTgcg  >  1:380473/1‑100 (MQ=255)
                                                                                                |                                                                                               
AATTGACTGGAATTTGGGTTTCGAGGCTCTTTGTGCTAAACTGGCCCGCCGAATGTATAGTACACTTCGGTTGGATAGGTAATTTGGCGAGATAATACGATGATCAAACAAAGGACACTTAAACGTATCGTTCAGGCGACGGGTGTCGGTTTACATACCGGCAAGAAAGTCACCCTGACGTTACGCCCTGCG  >  NC_000913/106458‑106649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: