| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 555,549 | T→C | R313R (CGT→CGC) | cysS → | cysteinyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 555,549 | 0 | T | C | 95.6% | 144.6 / ‑4.6 | 45 | R313R (CGT→CGC) | cysS | cysteinyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (23/20); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGC > NC_000913/555453‑555644 | gAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGcgcgcgcg > 1:578512/1‑100 (MQ=255) aTACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCgcggcg > 1:27731/1‑100 (MQ=255) actacGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCt > 1:629897/1‑100 (MQ=255) ctacGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTg < 1:1156810/100‑1 (MQ=255) tacGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTgg < 2:1057599/100‑1 (MQ=255) acgCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCg < 2:574566/100‑1 (MQ=255) aaaCCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTAAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTa < 2:300824/100‑1 (MQ=255) tGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACActgc < 1:522183/100‑1 (MQ=255) tCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTTTTGTTGGCGGGTGCGGCGCCCGAGGAGATCTACACTGCGCTGCGCgg < 2:749690/100‑1 (MQ=255) ccTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGc > 1:997994/1‑100 (MQ=255) ccTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGc > 2:176764/1‑100 (MQ=255) ccTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGc > 1:514542/1‑100 (MQ=255) ccTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGc < 1:433299/100‑1 (MQ=255) cTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGca > 1:43662/1‑100 (MQ=255) cTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGca > 1:432003/1‑100 (MQ=255) cTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGca > 1:249975/1‑100 (MQ=255) gATGGCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGcaca < 1:517509/100‑1 (MQ=255) tCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATa < 2:1129647/100‑1 (MQ=255) cGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATaa > 1:862384/1‑100 (MQ=255) gCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAAc < 2:608343/100‑1 (MQ=255) ccACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAAcc > 1:533688/1‑100 (MQ=255) ccACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAAcc > 1:26507/1‑100 (MQ=255) cACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCg > 2:1294768/1‑100 (MQ=255) tATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTg < 2:1251295/100‑1 (MQ=255) aTCGCAGCCAGTTGGACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTgc > 1:34007/1‑100 (MQ=255) cGCAGCCAGTTGAACTACAGAGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTgcgc > 2:55660/1‑100 (MQ=255) gCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGcc > 2:265309/1‑100 (MQ=255) cAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACATTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCg < 2:801401/100‑1 (MQ=255) cAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCg > 2:361350/1‑100 (MQ=255) cAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCg > 2:146902/1‑100 (MQ=255) cAGTTGAACGACAGCGAAGAGATCCTGAAGCGGGCGGGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGcc < 2:85666/99‑1 (MQ=255) ttGAACTACAGCGAAGAGAACCTGAAGAAGGCGCGCGCGGCGCTGAAGCGTCTCTACACAGCACTCCGCAGCACAGATAAAACCGTTGCGcccac > 2:12655/1‑92 (MQ=255) aaCTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCg < 1:1003891/100‑1 (MQ=255) aCTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGa < 1:710905/100‑1 (MQ=255) cTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGaa > 2:748406/1‑100 (MQ=255) cTACAGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGaa > 1:1164444/1‑100 (MQ=255) aGCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCg > 1:153406/1‑99 (MQ=255) gCGAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGtt > 1:1006441/1‑100 (MQ=255) gAAGAGAACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTg < 1:1059205/100‑1 (MQ=255) aaGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGa > 2:638208/1‑100 (MQ=255) gagaACCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAg < 1:265312/100‑1 (MQ=255) aCCTGAAGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCGGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAgcgcg < 2:487574/100‑1 (MQ=255) aGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTAt < 1:830363/100‑1 (MQ=255) aGCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTAt > 1:1068022/1‑100 (MQ=255) gCAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTAtt < 2:1041709/100‑1 (MQ=255) cAGGCGCGCGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTg < 2:508867/100‑1 (MQ=255) gcgcgcgcGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAg < 2:249972/100‑1 (MQ=255) cgcgcgcgGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGc > 1:1167398/1‑100 (MQ=255) cgcgcgcgGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGc < 2:997985/100‑1 (MQ=255) | GAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCGCGCTTTATTGAAGC > NC_000913/555453‑555644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |