| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 658,153 | A→G | pseudogene (123/195 nt) | ybeM → | pseudogene, C‑N hydrolase superfamily homology;putative enzyme; Not classified; putative amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 658,153 | 0 | A | G | 94.4% | 167.2 / ‑0.7 | 54 | pseudogene (123/195 nt) | ybeM | pseudogene, C‑N hydrolase superfamily homology;putative enzyme; Not classified; putative amidase |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (25/26); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
GTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTAAA > NC_000913/658058‑658248 | gTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGc < 2:404719/100‑1 (MQ=255) tACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTCGTGGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTg > 2:850469/1‑100 (MQ=255) tCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGgcgc > 1:756746/1‑100 (MQ=255) tgtgtgGGAAAAGAACGGTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGgcgcgc < 2:1229831/100‑1 (MQ=255) gtgtgGGAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGcgcgcg < 1:828582/100‑1 (MQ=255) tgtgGGAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCga < 2:305892/100‑1 (MQ=255) ggAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCgatgat < 1:852178/100‑1 (MQ=255) gAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGAGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATc > 1:314311/1‑100 (MQ=255) aaaGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCa > 1:702049/1‑99 (MQ=255) aGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCatga > 2:875037/1‑100 (MQ=255) gAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCatgat > 2:297922/1‑100 (MQ=255) cGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCa > 2:930258/1‑100 (MQ=255) gCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAg < 2:1124145/100‑1 (MQ=255) cTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGa < 2:202801/100‑1 (MQ=255) cTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGa > 1:742926/1‑100 (MQ=255) cTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGa > 1:1243252/1‑100 (MQ=255) gagaTTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATc > 1:356899/1‑100 (MQ=255) gagaTTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATc > 2:32187/1‑100 (MQ=255) gaTTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTa > 2:725408/1‑100 (MQ=255) tgtgCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCgg < 1:326346/100‑1 (MQ=255) tgtgCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCgg < 2:194125/100‑1 (MQ=255) tgCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGtt < 1:1210824/100‑1 (MQ=255) tgCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGtt > 2:1049859/1‑100 (MQ=255) gCCTCGGTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGGTa > 2:986712/1‑100 (MQ=255) cTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTaa > 2:725261/1‑99 (MQ=255) ttGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAg < 1:1260276/100‑1 (MQ=255) gcgcAGGCGGCGGAAAACGACGCTTGGCTGTTTGCACTGCCGGAAGAGTGGCTGGCGCGCGATGATCGTTATTCAGATCTGTCGGTTAAATCAGCagag > 2:1300751/1‑99 (MQ=255) gcggcgGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTgg < 1:32188/100‑1 (MQ=255) cggcggAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGa < 2:158028/100‑1 (MQ=255) cggcggAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGa < 2:658412/100‑1 (MQ=255) gcggAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAg < 2:849232/100‑1 (MQ=255) gcggAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAg < 2:1274209/100‑1 (MQ=255) cggAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAgg > 1:167412/1‑100 (MQ=255) cggAAAACGACGCATCGCTGTTTGCCCTGCCGAAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGaaag > 2:164117/1‑98 (MQ=255) gAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCg > 1:909220/1‑100 (MQ=255) gAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCg < 1:987647/100‑1 (MQ=255) aaaCGACGCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGaa < 2:998489/100‑1 (MQ=255) acgCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCt > 1:1120372/1‑100 (MQ=255) cgCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTc > 2:387182/1‑100 (MQ=255) cgCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTc < 1:227381/100‑1 (MQ=255) gCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCg < 1:1097871/100‑1 (MQ=255) gCATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCg < 2:715972/100‑1 (MQ=255) cATCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCgg > 2:812033/1‑100 (MQ=255) tCGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGAc < 1:328721/100‑1 (MQ=255) cGCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACt < 1:461042/100‑1 (MQ=255) gCTGTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACtt > 1:1226674/1‑100 (MQ=255) gTTTGCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTAc < 1:812678/100‑1 (MQ=255) gCCCTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCg > 2:62018/1‑100 (MQ=255) ccTGCCGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCgag > 1:286602/1‑100 (MQ=255) cGGAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGt < 1:329960/100‑1 (MQ=255) cGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAg < 2:744837/99‑1 (MQ=255) ggAAGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTa < 2:314308/100‑1 (MQ=255) aaGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTaaa > 1:252494/1‑100 (MQ=255) aaGCGTTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTaaa < 1:666197/100‑1 (MQ=255) | GTTACATCTGTGTGGGAAAAGAACGCTGAGATTTGTGCCTCGTTGATGGCGCAGGCGGCGGAAAACGACGCATCGCTGTTTGCCCTGCCGGAAGCATTGCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACTTTACGGCGAGAAAGTAAA > NC_000913/658058‑658248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |