| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 676,403 | C→T | A49T (GCC→ACC) | ybeQ ← | Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 676,403 | 0 | C | T | 98.0% | 163.1 / ‑4.1 | 50 | A49T (GCC→ACC) | ybeQ | Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (27/22); total (28/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
TTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGA > NC_000913/676309‑676498 | ttATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGtttttt < 1:412833/100‑1 (MQ=255) tATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTtc < 2:1281341/100‑1 (MQ=255) aTGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTATAAGGTTTTTtcg < 1:858735/100‑1 (MQ=255) gACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTtcgtc > 2:1228258/1‑100 (MQ=255) gACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTtcgtc > 1:285133/1‑100 (MQ=255) gCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTcc > 1:194926/1‑100 (MQ=255) cTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCg < 1:378841/100‑1 (MQ=255) ttATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGgag > 1:786152/1‑100 (MQ=255) ccTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATc > 1:852489/1‑100 (MQ=255) cTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCa > 2:255833/1‑100 (MQ=255) cGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAAt < 2:260667/100‑1 (MQ=255) gTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTg > 1:51835/1‑100 (MQ=255) ataCTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTgcg < 1:13397/100‑1 (MQ=255) taCTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTgcgc < 1:1268649/100‑1 (MQ=255) cTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAAACAATTGCGCTa > 1:464970/1‑100 (MQ=255) ttGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATcg < 2:568561/100‑1 (MQ=255) tGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATcgc < 2:115812/100‑1 (MQ=255) gCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATcgcg > 1:1148389/1‑100 (MQ=255) cccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATcgc > 2:469480/1‑98 (MQ=255) cccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGa > 2:539506/1‑100 (MQ=255) ccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAt < 1:137709/100‑1 (MQ=255) ccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAt > 2:44297/1‑100 (MQ=255) ccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAt < 2:595199/100‑1 (MQ=255) ccTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAt < 2:595231/100‑1 (MQ=255) cTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGAt < 2:175316/99‑1 (MQ=255) ggAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTataa > 2:991805/1‑100 (MQ=255) tACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATaaa > 1:419307/1‑100 (MQ=255) cTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAAc < 1:105494/100‑1 (MQ=255) tCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCcaacaa < 1:1228269/100‑1 (MQ=255) cacaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAat > 1:377498/1‑100 (MQ=255) acaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAACACCCAACAata < 2:1220989/100‑1 (MQ=255) acaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAata < 1:1024186/100‑1 (MQ=255) acaATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAata < 2:117048/100‑1 (MQ=255) caATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAatat > 1:1212373/1‑100 (MQ=255) gACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTg > 2:216176/1‑100 (MQ=255) cTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGACTCAATTGCGATATCGCGATTATAATAAAACCCAAAAATATATTgcgc > 2:429203/1‑100 (MQ=255) aGCGGCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCaa > 1:1262241/1‑100 (MQ=255) ggCCAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCg > 1:1034034/1‑100 (MQ=255) ccAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGcc > 2:197821/1‑100 (MQ=255) cAGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCt > 2:891463/1‑100 (MQ=255) aGCTTCAGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCtt > 1:390991/1‑100 (MQ=255) aGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAg < 1:1063986/100‑1 (MQ=255) aGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAg > 1:27517/1‑100 (MQ=255) aGCCAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAg < 2:1004548/100‑1 (MQ=255) gCCAGTAAAAGGTTTTTTGGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc > 1:736575/1‑100 (MQ=255) gCCAGTAAAAGGTTTTTTCGTCGTCCGTAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGc > 2:526312/1‑100 (MQ=255) ccAGTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCa < 2:1212362/100‑1 (MQ=255) gTAAAAGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGt > 1:745968/1‑100 (MQ=255) aaaaGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTc < 1:854355/100‑1 (MQ=255) aaaGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCg < 1:1029577/100‑1 (MQ=255) aaGGTTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGa > 2:684306/1‑100 (MQ=255) | TTATGACGGCTTTTATCCTCGGTATACTTTTGCCCTAAGGAATACTGTGCTTCACAATGACCTTGCTCAGCGGCCAGCTTCAGCCAGTAAAAGGCTTTTTCGTCGTCCGGAGAATCAATTGCGCTATCGCGATTATAATAAAACCCAACAATATATTGAGCCTCGCAATCGCCTTTTTCAGCACGTTCGA > NC_000913/676309‑676498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |