Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 720,946 C→T intergenic (+106/+110) ybfK → / ← kdpE uncharacterized protein/response regulator in two‑component regulatory system with KdpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913720,9460CT98.4% 200.3 / ‑4.2 63intergenic (+106/+110)ybfK/kdpEuncharacterized protein/response regulator in two‑component regulatory system with KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (33/29);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

AAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTA  >  NC_000913/720849‑721042
                                                                                                 |                                                                                                
aaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATTTTAATTTATTTAATGATGAGCTTTTTACTTAg                                                                                                <  1:1237886/100‑1 (MQ=255)
 aGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGt                                                                                               >  1:200304/1‑100 (MQ=255)
    tGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAat                                                                                            >  2:1053576/1‑100 (MQ=255)
       aGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAAtata                                                                                         <  2:733706/100‑1 (MQ=255)
          tatTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATaaaa                                                                                      <  2:48584/100‑1 (MQ=255)
              cTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATAtt                                                                                  >  1:301424/1‑100 (MQ=255)
               tATCAAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTg                                                                                 <  2:400369/100‑1 (MQ=255)
                    aGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTg                                                                            <  2:375845/100‑1 (MQ=255)
                    aGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTg                                                                            >  1:1271764/1‑100 (MQ=255)
                      gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGt                                                                           <  1:1054599/99‑1 (MQ=255)
                      gAAATACGGTTGAACTGTGAAGGTCATCAATAAAAAATGATCAATCATAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTg                                                                            <  2:1039114/98‑1 (MQ=255)
                       aaaTACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTa                                                                         <  1:598875/100‑1 (MQ=255)
                           aCGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTAtttt                                                                     >  2:1191221/1‑100 (MQ=255)
                            cGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTATTTTTTTTTTTAAAATATTGAATTGTTAttttt                                                                    >  2:17927/1‑100 (MQ=255)
                             ggTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtg                                                                   >  2:1195370/1‑100 (MQ=255)
                              gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTAttttt                                                                    >  2:28727/1‑98 (MQ=255)
                              gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtgt                                                                  <  2:462467/100‑1 (MQ=255)
                              gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtgt                                                                  >  1:930584/1‑100 (MQ=255)
                               ttGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtgtg                                                                 <  1:559290/100‑1 (MQ=255)
                               ttGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtgtg                                                                 <  2:722659/100‑1 (MQ=255)
                               ttGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtgtg                                                                 <  1:737382/100‑1 (MQ=255)
                               ttGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTtgtg                                                                 <  2:1048378/100‑1 (MQ=255)
                               ttGAACTGTGAAGATGATAAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtgtg                                                                 <  2:155669/100‑1 (MQ=255)
                                    cTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTATTTAGTAATATAAAATATTGAATTGGTTTTTTtgtg                                                                 >  2:1171079/1‑95 (MQ=255)
                                    cTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGtgttg                                                            <  1:709086/100‑1 (MQ=255)
                                    cTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGtgttg                                                            <  1:708923/100‑1 (MQ=255)
                                      gtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGtgttgt                                                           >  2:337384/1‑99 (MQ=255)
                                      gtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTATTGTGTgttgtt                                                          >  1:1208698/1‑100 (MQ=255)
                                      gtgAAGGTGAACAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTATTAATATAAAATATTGAATTGTTATTTTTGTGTgttgtt                                                          >  2:892169/1‑100 (MQ=255)
                                         aaGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTaa                                                       <  1:164467/100‑1 (MQ=255)
                                           ggTGATCAATAAAAAATGATCAATCTTAATTTATTTGATGATGAGCTTATTATGTATTAATATAAAATATTTAATTGTTATTTTTATGTTTAATTTAAGa                                                     >  2:489593/1‑100 (MQ=255)
                                            gTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTATTTAatat                                                    >  2:1186524/1‑100 (MQ=255)
                                             tGATCAATAATAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATCTTGAATTGTTATTTTTGTGTGTTGTTTAAGATa                                                   <  2:853821/100‑1 (MQ=255)
                                             tGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGAt                                                    <  2:562760/99‑1 (MQ=255)
                                             tGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATa                                                   <  2:1193207/100‑1 (MQ=255)
                                             tGACCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATCTTTGTGTGTTGTTTAAGATa                                                   >  2:16407/1‑100 (MQ=255)
                                               aTCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTaa                                                       >  2:823374/1‑94 (MQ=255)
                                               aTCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATaaa                                                 >  2:51255/1‑100 (MQ=255)
                                                tCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATaaaa                                                <  2:938292/100‑1 (MQ=255)
                                                 cAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATaaaaa                                               >  1:425302/1‑100 (MQ=255)
                                                  aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAg                                              >  2:490725/1‑100 (MQ=255)
                                                     aaaaaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCg                                           <  2:1009980/100‑1 (MQ=255)
                                                       aaaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGta                                         >  2:1027465/1‑100 (MQ=255)
                                                        aaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtat                                        >  2:606558/1‑100 (MQ=255)
                                                        aaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtat                                        >  1:484077/1‑100 (MQ=255)
                                                                   cTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACg                             <  1:217403/100‑1 (MQ=255)
                                                                      aaTTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                          <  1:1195381/100‑1 (MQ=255)
                                                                       atttatttAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCtt                         >  1:1017220/1‑100 (MQ=255)
                                                                        tttatttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                        >  1:1021543/1‑100 (MQ=255)
                                                                          tatttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaa                      <  1:708565/100‑1 (MQ=255)
                                                                           atttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaat                     >  2:190559/1‑100 (MQ=255)
                                                                            tttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaatt                    <  1:606562/100‑1 (MQ=255)
                                                                            ttaaaTGATGAGCTTTGTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaatt                    <  1:892176/97‑1 (MQ=255)
                                                                             ttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaatta                   <  1:70469/100‑1 (MQ=255)
                                                                              taATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaattaa                  >  1:312829/1‑100 (MQ=255)
                                                                              taATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaattaa                  >  1:1269064/1‑100 (MQ=255)
                                                                              taATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaattaa                  <  2:900948/100‑1 (MQ=255)
                                                                              taATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaattaa                  >  1:1268945/1‑100 (MQ=255)
                                                                                        cTTTTTACTTAGTAATATAAGATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggca         >  2:799607/1‑99 (MQ=255)
                                                                                            ttACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGTCTg    >  2:1080387/1‑100 (MQ=255)
                                                                                            ttACTTAGTAATATAAAATATTAAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTg    <  2:1208687/100‑1 (MQ=255)
                                                                                              aCTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTa  >  2:401446/1‑100 (MQ=255)
                                                                                              aCTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTa  >  2:494268/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTA  >  NC_000913/720849‑721042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑