| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 849,405 | G→A | T231T (ACC→ACT) | rhtA ← | threonine and homoserine efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 849,405 | 0 | G | A | 98.0% | 172.7 / ‑4.1 | 50 | T231T (ACC→ACT) | rhtA | threonine and homoserine efflux system |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (22/27); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
GTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTGCTTC > NC_000913/849313‑849500 | gtCAGTGTTTCTCCGAGGAAAATCATCCCGGACACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGCGAgg > 2:252293/1‑99 (MQ=255) tCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGc < 1:807649/100‑1 (MQ=255) cAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCa < 1:1202843/100‑1 (MQ=255) aGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCaa > 2:364860/1‑100 (MQ=255) tttCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCAt > 1:150004/1‑100 (MQ=255) ttCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCAtt < 2:945833/100‑1 (MQ=255) gAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGc < 1:127494/100‑1 (MQ=255) aaaaTCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAAt < 2:386944/100‑1 (MQ=255) tcatcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAgg < 1:389582/100‑1 (MQ=255) catcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGc < 1:863286/100‑1 (MQ=255) catcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGc > 2:554953/1‑100 (MQ=255) catcCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGc < 2:323333/100‑1 (MQ=255) gAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTCCCAGCGAATAAGGCAGAGCgg > 2:1101433/1‑100 (MQ=255) aaCGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTc < 1:1039012/100‑1 (MQ=255) aaCGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTc < 2:1225112/100‑1 (MQ=255) gCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgaga < 1:329983/100‑1 (MQ=255) agccagtGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagaga > 2:958001/1‑100 (MQ=255) gccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCgagag > 1:303670/1‑98 (MQ=255) ccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAAt < 2:219828/100‑1 (MQ=255) ccagcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAAt > 2:628893/1‑100 (MQ=255) ccagcGCCGGTTCCATGCTCATCAGCATACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAAt > 1:793401/1‑100 (MQ=255) agcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCGTTTCCAGCGAATAAGGCAGAGCGGCCGAGAGaa > 2:720093/1‑97 (MQ=255) agcGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAg < 2:497624/100‑1 (MQ=255) cgcCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCg > 1:559737/1‑100 (MQ=255) gcCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGa < 1:554957/100‑1 (MQ=255) cGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACa > 1:726762/1‑100 (MQ=255) ttCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGcc < 1:5723/100‑1 (MQ=255) ttCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGcc < 1:389638/100‑1 (MQ=255) cATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGa > 2:6501/1‑100 (MQ=255) aTGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAc < 1:531128/100‑1 (MQ=255) tGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAcc > 2:1293161/1‑100 (MQ=255) tGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAcc < 1:689726/100‑1 (MQ=255) gCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGAccc < 1:314501/100‑1 (MQ=255) tcatcaGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCaa > 1:1264949/1‑100 (MQ=255) catcaGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAAt > 2:627898/1‑100 (MQ=255) atcaGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATg < 1:364863/100‑1 (MQ=255) atcaGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATg < 1:1249012/100‑1 (MQ=255) atcaGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATg < 1:1249213/100‑1 (MQ=255) caGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGa > 2:110087/1‑100 (MQ=255) caGCGTACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGa < 1:1163298/100‑1 (MQ=255) tACCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAAc < 1:272065/100‑1 (MQ=255) aCCAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAAcc < 1:1293655/100‑1 (MQ=255) ccAAATGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCg > 1:902505/1‑100 (MQ=255) aaTGTCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGAcc > 2:769022/1‑100 (MQ=255) tCCGTGTTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGGATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTg < 1:669363/100‑1 (MQ=255) tgtTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCag < 1:33920/100‑1 (MQ=255) tgtTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCag > 1:1138233/1‑100 (MQ=255) tgtTGGCAAACGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCag > 1:1100784/1‑100 (MQ=255) aaaCGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTGCtt > 1:812170/1‑100 (MQ=255) aaCGAGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTGCTTc > 1:779926/1‑100 (MQ=255) | GTCAGTGTTTCTCCGAGGAAAATCATCCCGGAAACGGCAGCCAGCGCCGGTTCCATGCTCATCAGCGTACCAAATGTCCGTGTTGGCAAACGGGTGAGGGCAATCATTTCCAGCGAATAAGGCAGAGCGGTCGAGAGAATAGCGACAGCCAGACCCAATGGAATAACCGACCAGTGCCAGAGTGCTTC > NC_000913/849313‑849500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |