| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 873,807 | G→A | G277S (GGT→AGT) | yliE → | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 873,807 | 0 | G | A | 96.3% | 180.8 / ‑1.7 | 54 | G277S (GGT→AGT) | yliE | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (27/25); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
TATAATATTATTGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGT > NC_000913/873711‑873901 | tataATATTATTGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTa > 2:861902/1‑100 (MQ=255) atatTATTGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAAttt > 1:1182860/1‑100 (MQ=255) atatTATTGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAAttt > 1:364634/1‑100 (MQ=255) tattatTGATTCTGTCGATACTCAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTa > 2:759660/1‑100 (MQ=255) attattGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTAt < 2:246694/100‑1 (MQ=255) tGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAAc > 1:797444/1‑100 (MQ=255) gATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACg < 1:948063/100‑1 (MQ=255) cTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATc > 2:1034405/1‑100 (MQ=255) tGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCa < 1:937709/100‑1 (MQ=255) tGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCa > 1:973664/1‑100 (MQ=255) tGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCa < 2:256451/100‑1 (MQ=255) tCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGa < 1:982304/100‑1 (MQ=255) gATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATa > 2:303864/1‑100 (MQ=255) aTACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATaa < 1:14038/100‑1 (MQ=255) cTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACt > 1:456100/1‑100 (MQ=255) aaTATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACtttt < 1:1073475/100‑1 (MQ=255) tatatCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACtttttt < 1:1036419/100‑1 (MQ=255) cGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTg > 1:133455/1‑100 (MQ=255) cGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTg > 1:537608/1‑100 (MQ=255) aaaCATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGtt < 1:26944/100‑1 (MQ=255) aTCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCg < 1:768539/100‑1 (MQ=255) cAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGtt < 1:1034415/100‑1 (MQ=255) ggTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCatt < 1:759665/100‑1 (MQ=255) tGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCattat > 2:12362/1‑100 (MQ=255) tGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCattat > 2:971149/1‑100 (MQ=255) tGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCattat < 1:351450/100‑1 (MQ=255) gCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTGATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCattatt > 2:725624/1‑100 (MQ=255) gCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCattatt < 1:1133936/100‑1 (MQ=255) aaaCAACGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATc > 1:928506/1‑100 (MQ=255) acGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTAtt > 2:44312/1‑100 (MQ=255) cGCATTATTCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTg > 1:596128/1‑100 (MQ=255) cATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCt > 1:623050/1‑100 (MQ=255) ttattCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTTTTTTTTTTTTTCCTTCATTAGTATCGTCTTGCt > 2:451051/1‑98 (MQ=255) ttattCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCtgg < 1:1006523/100‑1 (MQ=255) ttattCGAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCtgg < 1:574092/100‑1 (MQ=255) gAAATCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGttt < 2:420832/100‑1 (MQ=255) aaTCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTGTTGCTGGTGGtttat > 2:475695/1‑100 (MQ=255) aaTCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGtttat < 1:12362/100‑1 (MQ=255) aTCTATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGtttatt < 2:133453/100‑1 (MQ=255) tATTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTaa > 2:401689/1‑100 (MQ=255) aTTTTTATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAAt < 2:747046/100‑1 (MQ=255) tttttATTATTCTGGCAGTAGTTTATTGAGGGCGACAGCTAAACTTTTTTATCTGCCGTTTGGGTTGATTATTCTCGTATTACGGGTGCgttattggatg > 2:623613/1‑95 (MQ=255) tttttATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTTTTTTTTTTTTTCGTTCAATACTACCTTATTGCTGCTGGtttatttatt > 2:903403/1‑97 (MQ=255) tttttATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATg < 1:980117/100‑1 (MQ=255) tttttATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATg > 2:1090580/1‑100 (MQ=255) tttttATTATCCTGGCAGTAATTTATTGAACGCATCAGATAAAATTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATg < 2:832417/100‑1 (MQ=255) tatCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTCTTTATCTGCCGTTTGCGTTCATTATTATAGTATTGCTGGTGGTTTATTTAATGACCACTc > 2:847677/1‑100 (MQ=255) atCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCg < 2:417784/100‑1 (MQ=255) atCCTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGCTTTATTTAATGACCACTCg > 2:734931/1‑100 (MQ=255) ccTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCgtg < 1:44653/100‑1 (MQ=255) ccTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCgtg < 1:1083249/100‑1 (MQ=255) ccTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCgtg > 2:894355/1‑100 (MQ=255) ccTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCgtg > 1:211642/1‑100 (MQ=255) cTGGCAGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCgtgt > 2:304417/1‑100 (MQ=255) | TATAATATTATTGATTCTGTCGATACTGAATATATCGAAAAAACATCAGCGGTGCCAAACAACGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGT > NC_000913/873711‑873901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |