| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 875,714 | T→G | V127G (GTC→GGC) | yliF → | putative membrane‑anchored diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 875,714 | 0 | T | G | 88.1% | 115.2 / 1.3 | 44 | V127G (GTC→GGC) | yliF | putative membrane‑anchored diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (2/0); major base G (18/19); minor base A (0/5); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCCTTATCTGATTTCCAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGTCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGATATTTTTGCTTGGTC > NC_000913/875618‑875809 | gTCCTTATCTGATTTCCAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCaa > 2:693038/1‑100 (MQ=255) ccTTATCTGATTTCCAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCaaaa < 2:824200/100‑1 (MQ=255) tGATTTCCAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAgg < 2:173003/100‑1 (MQ=255) ttCCAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGttt < 2:498203/100‑1 (MQ=255) tCCAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTa < 1:216015/100‑1 (MQ=255) cAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTAtc > 2:936077/1‑100 (MQ=255) cAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTAtc < 1:226195/100‑1 (MQ=255) aGTGACGAATGTATAAAGGTAAAGGAGTTCGAGAAAAATAATTGTGAAGATATTATGCAGGTTGTGAAGTAAAAAAAAAAAAAAAATACAGGGTTTAtct < 2:943135/100‑1 (MQ=255) gACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCtt > 1:604831/1‑100 (MQ=255) aCGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCttt < 1:705515/100‑1 (MQ=255) cGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCtttt > 1:894389/1‑100 (MQ=255) cGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCtttt < 1:521593/100‑1 (MQ=255) gAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATAAAAAAAAAAAAAACACAGGGTTTATCTCTTTTg < 2:928103/100‑1 (MQ=255) aTTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTa < 1:938184/100‑1 (MQ=255) aTTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTa < 2:24550/100‑1 (MQ=255) aTTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTa > 2:729704/1‑100 (MQ=255) aGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAAc < 1:727582/100‑1 (MQ=255) tAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCtt > 1:560622/1‑100 (MQ=255) tAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCtt > 1:1267442/1‑100 (MQ=255) aaGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTAAAAAAAAAAAAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCg < 2:886960/100‑1 (MQ=255) gTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTAt > 2:742735/1‑100 (MQ=255) gTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTAt > 2:1285781/1‑100 (MQ=255) tCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTa < 1:1290020/100‑1 (MQ=255) gagaAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTAcc < 2:471635/100‑1 (MQ=255) gaAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACctc > 2:1075761/1‑100 (MQ=255) aaTTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATc > 1:1178577/1‑100 (MQ=255) tattGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCgg < 1:374472/100‑1 (MQ=255) ttGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTa < 1:396212/100‑1 (MQ=255) tgtgCAGATATTATGCAGGTTGTCAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTaa > 2:290196/1‑100 (MQ=255) gtgCAGATATTATGCAGGTTGTGAAGTATCGACATAGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAAc < 2:440210/100‑1 (MQ=255) cAGATATTATGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTca < 2:894382/100‑1 (MQ=255) aGATATTATGCAGGTTGTGAAGTAAAAAAAAAAAAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTcac < 2:149284/100‑1 (MQ=255) ttatGCAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCg > 1:189500/1‑100 (MQ=255) tatGCAGGTTGTGAAGTAAAAAAAAAAAAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGt < 2:1110900/100‑1 (MQ=255) cAGGTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTc > 2:1139614/1‑100 (MQ=255) ggTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTg > 1:817908/1‑100 (MQ=255) ggTTGTGAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTg < 2:44986/100‑1 (MQ=255) gtgAAGTATCGACATGTCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGatat > 1:147137/1‑100 (MQ=255) tgAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGATAtt > 1:617859/1‑100 (MQ=255) gAAGTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGATAttt > 1:701659/1‑100 (MQ=255) aGTATCGACATGTCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGatgcgaat > 1:425847/1‑94 (MQ=255) gTATCGACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCACTATCCGGTAACTCACAATCGTAGTCTGATATTTTTg < 2:700524/100‑1 (MQ=255) gACATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGAGATTTTGGCTTgg > 2:106979/1‑100 (MQ=255) cATGGCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGATATTTTTGCTTGGTc < 1:1169250/100‑1 (MQ=255) | GTCCTTATCTGATTTCCAGTGACGAATGTATTCAGGTAAAGGAGTTCGAGAAAAATTATTGTGCAGATATTATGCAGGTTGTGAAGTATCGACATGTCAAAAATACAGGGTTTATCTCTTTTGACGGTAAAACCTTCGTCTATTACCTCTATCCGGTAACTCACAATCGTAGTCTGATATTTTTGCTTGGTC > NC_000913/875618‑875809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |