Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 882,722 G→A R5C (CGC→TGC)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913882,7220GA97.9% 161.7 / ‑4.0 49R5C (CGC→TGC) deoRdeoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (24/23);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

GTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATC  >  NC_000913/882625‑882819
                                                                                                 |                                                                                                 
gTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAAc                                                                                                 >  2:128193/1‑100 (MQ=255)
  cATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACgt                                                                                               >  1:180640/1‑100 (MQ=255)
   aTCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACgtg                                                                                              <  2:797128/100‑1 (MQ=255)
       ccGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTc                                                                                          >  2:219840/1‑100 (MQ=255)
         gAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAACTGCCCGATACGCTCTTCGCAACGTGTTTCCa                                                                                        <  2:719952/100‑1 (MQ=255)
              cccAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATc                                                                                   >  1:473971/1‑100 (MQ=255)
                    cAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTg                                                                             <  1:1139506/100‑1 (MQ=255)
                    cAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTg                                                                             <  2:810515/100‑1 (MQ=255)
                    cAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTg                                                                             <  2:26247/100‑1 (MQ=255)
                      gggCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGaa                                                                           >  1:1190798/1‑100 (MQ=255)
                       ggcggcggcGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAAt                                                                          <  2:327674/100‑1 (MQ=255)
                          ggcggcgTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGt                                                                       <  2:338841/100‑1 (MQ=255)
                          ggcggcgTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGt                                                                       <  1:424991/100‑1 (MQ=255)
                            cggcgTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTa                                                                     <  1:1141047/100‑1 (MQ=255)
                              gcgTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTAtt                                                                   <  2:493439/100‑1 (MQ=255)
                                  cTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAg                                                               >  1:475826/1‑100 (MQ=255)
                                      aaGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAg                                                           >  1:964231/1‑100 (MQ=255)
                                       aGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGc                                                          <  2:1257961/100‑1 (MQ=255)
                                       aGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGc                                                          >  2:470693/1‑100 (MQ=255)
                                       aGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGc                                                          >  2:696509/1‑100 (MQ=255)
                                       aGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGc                                                          >  2:708230/1‑100 (MQ=255)
                                        gATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGcc                                                         >  1:812195/1‑100 (MQ=255)
                                        gATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGcc                                                         >  1:54934/1‑100 (MQ=255)
                                           gTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCt                                                      >  1:701194/1‑100 (MQ=255)
                                           gTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCt                                                      >  1:659592/1‑100 (MQ=255)
                                            tAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTc                                                     >  2:1163784/1‑100 (MQ=255)
                                                ttATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAAta                                                 <  1:893248/100‑1 (MQ=255)
                                                    cGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAAtactac                                             >  2:703489/1‑100 (MQ=255)
                                                      cTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTacac                                           >  2:241511/1‑100 (MQ=255)
                                                        gCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACtt                                         >  1:904388/1‑100 (MQ=255)
                                                           ttttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCTACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACttttt                                      >  2:662929/1‑100 (MQ=255)
                                                               aaTTCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCa                                  <  2:740161/100‑1 (MQ=255)
                                                                  tCTTGCAGCAGCTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCagag                               >  1:914952/1‑100 (MQ=255)
                                                                       cagcagCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAg                          <  1:77214/100‑1 (MQ=255)
                                                                        agcagcTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGt                         >  1:53928/1‑100 (MQ=255)
                                                                         gcagcTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTc                        <  2:1301350/100‑1 (MQ=255)
                                                                          cagcTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCa                       <  2:822364/100‑1 (MQ=255)
                                                                           agcTGCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCAc                      <  2:754140/100‑1 (MQ=255)
                                                                               gCCCGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCAc                  >  2:171531/1‑100 (MQ=255)
                                                                                cccGATACGCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCAcc                 <  1:988895/100‑1 (MQ=255)
                                                                                       cGCTCTTCGCAAAGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCtt          >  1:859423/1‑100 (MQ=255)
                                                                                        gCTCTTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCttt         >  1:1137020/1‑100 (MQ=255)
                                                                                         ctctTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTg        >  1:217323/1‑100 (MQ=255)
                                                                                         ctctTCGCAACGTGTGTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTg        <  2:679922/100‑1 (MQ=255)
                                                                                          tctTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGc       <  1:683380/100‑1 (MQ=255)
                                                                                           ctTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCg      <  1:1163795/100‑1 (MQ=255)
                                                                                           ctTCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCg      <  2:922461/100‑1 (MQ=255)
                                                                                            ttCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCgg     <  2:812190/100‑1 (MQ=255)
                                                                                             tCGCAACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCg      <  1:33903/98‑1 (MQ=255)
                                                                                               gcgACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATc  >  1:1149904/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
GTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATC  >  NC_000913/882625‑882819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: