| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 894,155 | T→G | D124E (GAT→GAG) | potF → | putrescine ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 894,155 | 0 | T | G | 97.7% | 145.2 / ‑3.5 | 43 | D124E (GAT→GAG) | potF | putrescine ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (19/23); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
GCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGC > NC_000913/894066‑894249 | gCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATa > 1:484166/1‑100 (MQ=255) ggAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAt > 1:1011126/1‑100 (MQ=255) ggAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAt > 1:773724/1‑100 (MQ=255) gTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTg > 1:229876/1‑100 (MQ=255) ttttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGc > 1:310144/1‑100 (MQ=255) tttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCt < 2:1011117/100‑1 (MQ=255) ttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTa > 2:830116/1‑100 (MQ=255) cAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGc > 2:1178714/1‑100 (MQ=255) gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGccc < 1:211923/100‑1 (MQ=255) ccGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCt < 2:1297429/100‑1 (MQ=255) ggACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg < 1:451040/100‑1 (MQ=255) cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTgg < 2:470046/100‑1 (MQ=255) cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTgg > 1:953375/1‑100 (MQ=255) aaaGCAGATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc < 2:313560/100‑1 (MQ=255) aGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACCCGAGCCCGACAATAAATTTGCTATGCCCTATCTGTGGGcga > 1:1035989/1‑100 (MQ=255) aaTTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCgacgac < 2:493963/100‑1 (MQ=255) ttGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCg < 1:907697/100‑1 (MQ=255) gAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTg < 2:527179/100‑1 (MQ=255) ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta < 1:901933/100‑1 (MQ=255) gaagacTCTCGTACCGGCACTGCTGACGCTGGTTGCCAAACACGCGCCCGAGACTCAATTTGCTCTGCTCTATATTTGGGCGACGACCGGGATTGGc < 2:107518/97‑1 (MQ=255) gaagaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCtat < 1:618769/100‑1 (MQ=255) agaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATaa > 2:306711/1‑100 (MQ=255) agaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCAACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTCGCtata > 2:614316/1‑99 (MQ=255) ctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTg < 1:1178725/100‑1 (MQ=255) cGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGAt < 1:781000/100‑1 (MQ=255) ccGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAg < 2:474544/100‑1 (MQ=255) cGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGt < 2:551804/100‑1 (MQ=255) gAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTa < 1:68563/100‑1 (MQ=255) aCTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTaaa < 1:314603/100‑1 (MQ=255) aCTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTaaa < 1:173640/100‑1 (MQ=255) ctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCgg > 2:645166/1‑100 (MQ=255) tgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGt > 2:829135/1‑100 (MQ=255) gAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTg > 1:186418/1‑100 (MQ=255) cTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCAGTGCTgg > 2:484024/1‑100 (MQ=255) ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc > 1:640250/1‑100 (MQ=255) ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc > 1:840776/1‑100 (MQ=255) gTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCg < 1:320059/100‑1 (MQ=255) tCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGa > 2:451320/1‑100 (MQ=255) tCGCCAAACACGAGCCCGACAATAAAGTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGa < 2:846424/100‑1 (MQ=255) cGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGa < 2:415734/99‑1 (MQ=255) gCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaaa > 2:512661/1‑100 (MQ=255) aaaCACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCgcgtttt < 2:240823/100‑7 (MQ=255) cacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACgcgc < 1:924016/100‑1 (MQ=255) | GCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGC > NC_000913/894066‑894249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |