Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,105,806 T→C intergenic (+81/‑14) ymdA → / → ymdB uncharacterized protein/O‑acetyl‑ADP‑ribose deacetylase; RNase III inhibitor during cold shock; putative cardiolipin synthase C regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,105,8060TC96.8% 204.2 / ‑2.0 62intergenic (+81/‑14)ymdA/ymdBuncharacterized protein/O‑acetyl‑ADP‑ribose deacetylase; RNase III inhibitor during cold shock; putative cardiolipin synthase C regulatory subunit
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (32/28);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

ACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTC  >  NC_000913/1105709‑1105899
                                                                                                 |                                                                                             
aCATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGgcgt                                                                                             <  2:931149/100‑1 (MQ=255)
 cATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTa                                                                                            >  1:80570/1‑100 (MQ=255)
  aTCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTaa                                                                                           >  1:1113843/1‑100 (MQ=255)
   tCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAag                                                                                          >  1:1264276/1‑100 (MQ=255)
   tCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAag                                                                                          <  2:441836/100‑1 (MQ=255)
    cGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGGCTGCTGTACACTTAAGAAACAAGAAGGCGTAagg                                                                                         >  2:1142705/1‑100 (MQ=255)
      aaTATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAaggag                                                                                       >  2:104950/1‑100 (MQ=255)
       atatAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAaggagg                                                                                      >  2:662430/1‑100 (MQ=255)
          taACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGt                                                                                   >  1:370517/1‑100 (MQ=255)
            aCTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTa                                                                                 >  2:1162430/1‑100 (MQ=255)
                attttCTAAACAACTCTCGTTTTTGCGCAGCAAAATCCAAATTAACCAAAAAGTCTTCTCTACACTTAAGAAACAAGCAGGCGTTAGGAGGCGTTATGaa                                                                             <  2:589572/98‑1 (MQ=255)
                 gTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGaaa                                                                            >  2:837972/1‑100 (MQ=255)
                      tGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAAcgcg                                                                       >  2:1123641/1‑100 (MQ=255)
                      tGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAAcgcg                                                                       <  1:1001837/100‑1 (MQ=255)
                          caacTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTAtt                                                                   <  1:1162441/100‑1 (MQ=255)
                             cTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAt                                                                >  1:151906/1‑100 (MQ=255)
                             cTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAt                                                                >  1:871188/1‑100 (MQ=255)
                              tCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAtg                                                               <  1:1040493/100‑1 (MQ=255)
                               cACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAtg                                                               >  2:580659/1‑99 (MQ=255)
                                 cGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAtgttg                                                            <  1:519314/100‑1 (MQ=255)
                                 cGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCAtgttg                                                            >  1:195351/1‑100 (MQ=255)
                                    tGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGc                                                         <  1:1189218/100‑1 (MQ=255)
                                    tGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGc                                                         <  1:980616/100‑1 (MQ=255)
                                      cTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAg                                                       >  1:547754/1‑100 (MQ=255)
                                         aGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAggg                                                     >  2:1290506/1‑99 (MQ=255)
                                           cAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGa                                                  >  2:334338/1‑100 (MQ=255)
                                           cAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGa                                                  <  1:1241538/100‑1 (MQ=255)
                                            aGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGat                                                 >  1:122188/1‑100 (MQ=255)
                                            aGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGat                                                 >  1:1075264/1‑100 (MQ=255)
                                            aGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGat                                                 >  1:649883/1‑100 (MQ=255)
                                             ttccgatctgATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGTCGTTATGAAAACGCGTATTCATGTCGTGCAGGGTGata                                                <  2:803858/91‑1 (MQ=255)
                                             gAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGata                                                >  1:845557/1‑100 (MQ=255)
                                                  aTGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGAAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTAc                                            >  2:820842/1‑99 (MQ=255)
                                                     cGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCaaa                                        <  2:80569/100‑1 (MQ=255)
                                                      gATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGataagatcggaa                                       >  1:803863/1‑91 (MQ=255)
                                                           aaCCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGcc                                  <  2:366965/100‑1 (MQ=255)
                                                             ccAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAAGGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGt                                >  2:916908/1‑100 (MQ=255)
                                                             ccAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGt                                >  1:724380/1‑100 (MQ=255)
                                                             ccAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGt                                >  1:640990/1‑100 (MQ=255)
                                                             ccAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGt                                >  1:403491/1‑100 (MQ=255)
                                                             ccAAAAAGCCTGCTGTACACCTAAGACACACGAAGGCGTAAGGAGGCGTTATAAACACGCGTATTCATGTTATGCAGGGTGCTATTAGAAAACTCGCCg                                 >  2:1198060/1‑99 (MQ=255)
                                                              cAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGtt                               >  1:48741/1‑100 (MQ=255)
                                                               aaaaaGCCTGCTGTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGttn                              <  2:845551/100‑2 (MQ=255)
                                                                   aGCCTGCTGTACACTTAAGAAACAATAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGAtgt                          <  2:1059920/100‑1 (MQ=255)
                                                                       tgctgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGAtt                      >  2:547782/1‑100 (MQ=255)
                                                                       tgctgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGAtt                      <  2:104680/100‑1 (MQ=255)
                                                                          tgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCCTGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATtgtg                   <  2:725750/100‑1 (MQ=255)
                                                                          tgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATtgtg                   <  2:1075254/100‑1 (MQ=255)
                                                                          tgTACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATtgtg                   <  2:535470/100‑1 (MQ=255)
                                                                          tctACACTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATtgtg                   <  2:793418/98‑1 (MQ=255)
                                                                             acacTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAAt                <  1:662434/100‑1 (MQ=255)
                                                                             acacTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAAt                <  1:1132395/100‑1 (MQ=255)
                                                                              cacTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATg               <  2:1264265/100‑1 (MQ=255)
                                                                               acTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGc              >  1:6731/1‑100 (MQ=255)
                                                                                cTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCg             >  1:1070465/1‑100 (MQ=255)
                                                                                cTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCg             <  2:451499/100‑1 (MQ=255)
                                                                                cTTAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCg             >  1:828250/1‑100 (MQ=255)
                                                                                 ttAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCgg            <  2:878328/100‑1 (MQ=255)
                                                                                  tAAGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGc           <  1:1208831/100‑1 (MQ=255)
                                                                                   aaGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCt          <  2:232701/100‑1 (MQ=255)
                                                                                   aaGAAACAAGAAGGCGTAACGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCt          <  1:1123651/100‑1 (MQ=255)
                                                                                    aGAAACAAGAAGGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTa         >  1:582321/1‑100 (MQ=255)
                                                                                           agaagGCGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTc  <  2:1113833/100‑1 (MQ=255)
                                                                                                 |                                                                                             
ACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTC  >  NC_000913/1105709‑1105899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: