| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,143,125 | C→T | D415N (GAC→AAC) | rne ← | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,143,125 | 0 | C | T | 97.9% | 159.0 / ‑4.0 | 47 | D415N (GAC→AAC) | rne | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (21/25); total (21/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAG > NC_000913/1143031‑1143218 | ggCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTtacgca > 2:1065111/1‑100 (MQ=255) caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGt > 1:796821/1‑100 (MQ=255) caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGt < 2:909825/100‑1 (MQ=255) gAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGcc < 2:403877/100‑1 (MQ=255) aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTCCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAg < 2:1070166/100‑1 (MQ=255) aTGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTAc < 1:173669/100‑1 (MQ=255) ggCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGGGACAGCGAATCGTTGTTACGCACGGTGCCAGTACCa > 1:86022/1‑100 (MQ=255) gCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAg < 1:1187225/100‑1 (MQ=255) gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaa < 2:492922/100‑1 (MQ=255) ngAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTAGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGaac > 2:700467/2‑100 (MQ=255) tCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAAcgcg < 2:94176/100‑1 (MQ=255) ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCgg < 1:741802/100‑1 (MQ=255) ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCgg < 2:277862/100‑1 (MQ=255) tGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGAc < 1:546828/100‑1 (MQ=255) tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg > 1:516416/1‑100 (MQ=255) tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg < 1:678081/100‑1 (MQ=255) tctcTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtg > 1:1038581/1‑100 (MQ=255) ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga > 2:497063/1‑100 (MQ=255) ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga > 2:813677/1‑100 (MQ=255) ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtga < 2:83205/100‑1 (MQ=255) tctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgat < 1:641131/100‑1 (MQ=255) ctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGtgatg > 1:1238138/1‑100 (MQ=255) ttCAGCGTTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGAc > 2:406548/1‑100 (MQ=255) cAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTg > 1:275644/1‑100 (MQ=255) aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTgg < 1:1111067/100‑1 (MQ=255) gcgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGa > 1:174663/1‑100 (MQ=255) gcgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGa > 2:619233/1‑100 (MQ=255) cgcTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGAt < 2:200704/100‑1 (MQ=255) tcttcttaGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAc > 1:684108/1‑100 (MQ=255) ttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCAccc < 2:1243344/100‑1 (MQ=255) tcttcGATCAGACGCAGAATAGAGAGCGACAGAGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCa > 1:1235895/1‑100 (MQ=255) aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACGACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg < 1:700471/100‑1 (MQ=255) aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg < 1:12711/100‑1 (MQ=255) aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg < 2:549376/100‑1 (MQ=255) aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg < 1:56142/100‑1 (MQ=255) tCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATgg < 1:574306/100‑1 (MQ=255) cAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATggg < 2:961120/100‑1 (MQ=255) cAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATggg > 2:24472/1‑100 (MQ=255) cGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCa < 2:39189/100‑1 (MQ=255) cAGAATAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg > 1:635699/1‑100 (MQ=255) aaTAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgcgc > 1:533895/1‑100 (MQ=255) aTAGAGAGCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCt > 1:666561/1‑100 (MQ=255) gagagCGACAGCGATTCGTTGTTACACACGGTGCCAGTACCAGAAAAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATAGGATCAGGCGCTGAc > 2:1228894/1‑100 (MQ=255) agCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg < 1:87411/100‑1 (MQ=255) gCGACAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGa > 1:256207/1‑100 (MQ=255) aCAGCGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAt > 2:520332/1‑100 (MQ=255) cGATTCGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTcc < 1:619237/100‑1 (MQ=255) cGTTGTTACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagcag < 2:1119453/100‑1 (MQ=255) | GGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAG > NC_000913/1143031‑1143218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |