Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,303,332 | T→G | V145G (GTG→GGG) | oppB → | oligopeptide ABC transporter permease |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,303,332 | 0 | T | G | 94.0% | 170.0 / 2.7 | 50 | V145G (GTG→GGG) | oppB | oligopeptide ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base T (1/2); new base G (21/26); total (22/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 |
TAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTG > NC_000913/1303237‑1303426 | tAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGtt < 2:1154894/100‑1 (MQ=255) tAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGtt < 1:736127/100‑1 (MQ=255) aGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTg < 1:622317/100‑1 (MQ=255) tGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCa < 1:1062093/100‑1 (MQ=255) cTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCAcc < 1:702638/100‑1 (MQ=255) tGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGGTTTGGGGTTGCAcc > 2:16098/1‑99 (MQ=255) gCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCatt < 2:240682/100‑1 (MQ=255) cGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCatta > 2:826980/1‑100 (MQ=255) aTTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAg > 2:442687/1‑100 (MQ=255) ttccGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCatta < 2:1303737/92‑1 (MQ=255) ccGAATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCAt < 1:114700/100‑1 (MQ=255) cGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATg < 2:189733/100‑1 (MQ=255) aTTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGata > 1:612138/1‑100 (MQ=255) aTTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGata < 1:1225070/100‑1 (MQ=255) aTTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGata < 1:1226551/100‑1 (MQ=255) ttAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGatat > 2:204748/1‑100 (MQ=255) aaacaaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTg > 1:200593/1‑100 (MQ=255) aaacaaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTg > 1:461296/1‑100 (MQ=255) acaaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCg < 1:929312/100‑1 (MQ=255) caaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGa < 1:747858/100‑1 (MQ=255) aaaacaCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGAt < 2:1303260/100‑1 (MQ=255) acCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCAttt < 1:174847/100‑1 (MQ=255) ccAAATGGGATTATACCGTGATGGTGCTGGCAATGACCGGGGTCGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCAtttt < 2:831929/100‑1 (MQ=255) cAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTg < 2:401939/100‑1 (MQ=255) aaTGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCa < 2:890718/100‑1 (MQ=255) aaTGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCa < 1:628427/100‑1 (MQ=255) tGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCAtt > 1:983007/1‑100 (MQ=255) ggACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTgg > 2:248684/1‑100 (MQ=255) ggACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTgg < 1:495614/100‑1 (MQ=255) ataCCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGcc < 2:1110013/100‑1 (MQ=255) taCCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCg > 2:337251/1‑100 (MQ=255) ccGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCggg > 2:704544/1‑100 (MQ=255) gATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGt > 2:15989/1‑100 (MQ=255) ggCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTg > 1:338271/1‑100 (MQ=255) gCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTgg < 1:339768/100‑1 (MQ=255) cTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGa < 1:1018225/100‑1 (MQ=255) tGGCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGaa < 1:946974/100‑1 (MQ=255) ggCAATGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCCATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAt > 1:1242424/1‑100 (MQ=255) gCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAtg > 1:104015/1‑100 (MQ=255) aaTGACCGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAAtggt > 2:338367/1‑100 (MQ=255) ccGGGGTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGgc > 2:941209/1‑100 (MQ=255) gggTTGTTATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCt < 1:338370/100‑1 (MQ=255) tgttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTaaa > 2:754284/1‑100 (MQ=255) tgttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTaaa < 2:842053/100‑1 (MQ=255) gttATCCCCAGTTTTGGGGTTGCACCCTTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAAt > 1:793908/1‑100 (MQ=255) gttATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAAt > 1:1280826/1‑100 (MQ=255) tATCCCCAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTc > 2:6568/1‑100 (MQ=255) ccccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATg > 2:1114760/1‑100 (MQ=255) cccAGTTTTGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGa < 1:630041/100‑1 (MQ=255) ttttGGGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTg < 1:6568/100‑1 (MQ=255) | TAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGGCGGTGGCTGGAATGGTGGGGCGCTTAAATTCATGATATTG > NC_000913/1303237‑1303426 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |