| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,505,561 | T→C | M174V (ATG→GTG) | ydcO ← | BenE family inner membrane putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,505,561 | 0 | T | C | 97.7% | 153.5 / ‑4.3 | 44 | M174V (ATG→GTG) | ydcO | BenE family inner membrane putative transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (22/21); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
AATCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTG > NC_000913/1505465‑1505655 | aaTCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACcgc < 1:1027458/100‑1 (MQ=255)aaTCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACcgc > 1:1074481/1‑100 (MQ=255) ggggTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCaa > 1:1200815/1‑100 (MQ=255) gTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTa > 2:1217828/1‑100 (MQ=255) gTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTa > 2:646965/1‑100 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCg > 2:808320/1‑100 (MQ=255) ataAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCa < 1:738728/100‑1 (MQ=255) ataAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCa > 1:270488/1‑100 (MQ=255) aGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAg > 1:1303862/1‑100 (MQ=255) gTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAgc > 2:50529/1‑100 (MQ=255) agaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCgcggcg < 2:1102561/100‑1 (MQ=255) aaCGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCa < 2:548649/100‑1 (MQ=255) ggTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACgg < 1:1194163/100‑1 (MQ=255) gTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGc > 1:1173582/1‑100 (MQ=255) acaacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTgg > 2:1245508/1‑97 (MQ=255) caTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTgccag > 2:130551/1‑100 (MQ=255) aTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTgccagc > 1:1003737/1‑100 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTgccagcc > 1:981698/1‑100 (MQ=255) aGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCAt > 2:42240/1‑100 (MQ=255) tgACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAg < 2:1226655/100‑1 (MQ=255) tgACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAg < 2:632177/100‑1 (MQ=255) gACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAGTTACCGCATGGCGCGTCGCAACGGCCTTGGTTGCCAGCCATACCAGc > 2:881187/1‑100 (MQ=255) gACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGc > 2:535181/1‑100 (MQ=255) ttccgatctccTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCa < 2:60379/91‑1 (MQ=255) aCAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCa < 1:1026565/100‑1 (MQ=255) aCGTCACCTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACa < 2:1250134/100‑1 (MQ=255) ccTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCaagatcggaa > 1:60380/1‑92 (MQ=255) ccTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACtt > 1:751166/1‑99 (MQ=255) ccTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTc < 2:751161/100‑1 (MQ=255) cTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTcc < 2:981690/100‑1 (MQ=255) cTTGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTcc < 1:1177142/100‑1 (MQ=255) tGCGCGATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCcac < 1:277232/100‑1 (MQ=255) cgcgATGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCcacac < 1:17710/100‑1 (MQ=255) aTGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACg < 1:795095/100‑1 (MQ=255) aTGACGATCACGATCCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACg > 1:363954/1‑100 (MQ=255) acgatcacgatcCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTaa > 1:1260854/1‑100 (MQ=255) cgatcacgatcCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTaaa > 2:957889/1‑100 (MQ=255) atcacgatcCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAAtt < 1:1217839/100‑1 (MQ=255) tcacgatcCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTg < 1:429229/100‑1 (MQ=255) acgatcCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGAc < 1:277968/100‑1 (MQ=255) acgatcCCAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGAc < 2:766896/100‑1 (MQ=255) ccAATAATCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCa > 2:152709/1‑100 (MQ=255) ataatCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGAc < 1:1121766/100‑1 (MQ=255) aatCACCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTg > 1:337198/1‑100 (MQ=255) | AATCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGCCAGCCATACCAGCAACATACTTCCACACAACGTAAATTGACCGTCCAGACTG > NC_000913/1505465‑1505655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |