| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,519,159 | A→T | T45S (ACA→TCA) | curA → | curcumin/dihydrocurcumin reductase, NADPH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,519,159 | 0 | A | T | 98.2% | 190.8 / ‑3.6 | 57 | T45S (ACA→TCA) | curA | curcumin/dihydrocurcumin reductase, NADPH‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (31/25); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01 | |||||||||||
CTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGC > NC_000913/1519063‑1519254 | cTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAg > 2:577995/1‑100 (MQ=255) gCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGttt > 1:769036/1‑100 (MQ=255) cTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTAt > 2:965109/1‑100 (MQ=255) tCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTAtt < 2:1281705/100‑1 (MQ=255) gTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTc > 2:901811/1‑100 (MQ=255) tCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTAATGCGCTCAGTTTATTTGt > 2:838748/1‑98 (MQ=255) ccACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTccc < 1:988459/100‑1 (MQ=255) ccACATGGCGCACCTGTTCCGGAGAATTTACGTCTTGAGGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTccc > 2:1279755/1‑100 (MQ=255) cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCt < 1:94693/100‑1 (MQ=255) cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCt > 1:10993/1‑100 (MQ=255) cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCt > 2:275624/1‑100 (MQ=255) acaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTg > 2:639017/1‑100 (MQ=255) acaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTg > 1:375333/1‑100 (MQ=255) cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCg < 2:769031/100‑1 (MQ=255) cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCg < 2:924981/100‑1 (MQ=255) cACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGta < 1:75506/100‑1 (MQ=255) ccTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGtata < 1:1044731/100‑1 (MQ=255) ccTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGtata < 1:1065847/100‑1 (MQ=255) cTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTatat < 2:1301518/100‑1 (MQ=255) ccGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTg > 1:1110863/1‑100 (MQ=255) ccGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTg < 1:948678/100‑1 (MQ=255) cGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTgg > 2:1166627/1‑100 (MQ=255) gagaATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGAc > 2:532887/1‑100 (MQ=255) gagaATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCGGGACCCGTATATGCGTGGAc < 2:798143/100‑1 (MQ=255) tttCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATg < 2:375330/100‑1 (MQ=255) tCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAg < 1:863569/100‑1 (MQ=255) cGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCg < 2:652564/100‑1 (MQ=255) cGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCg > 2:1100366/1‑100 (MQ=255) gaagaaGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGc < 1:893744/100‑1 (MQ=255) gaagaaGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGc > 1:285512/1‑100 (MQ=255) gaagaaGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGc > 2:111626/1‑100 (MQ=255) gaagaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCAt > 2:379386/1‑100 (MQ=255) tgatgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTAt < 1:763792/100‑1 (MQ=255) cttccgatctacacCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTAt < 1:93575/90‑1 (MQ=255) gCCACACCGGGTAAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGc > 1:854848/1‑100 (MQ=255) acacCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTAtagatcggaag > 2:93573/1‑90 (MQ=255) cGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGa > 2:1156636/1‑100 (MQ=255) cGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGa > 2:274203/1‑100 (MQ=255) gggTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTg < 2:315959/98‑1 (MQ=255) ggTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGata < 1:1168347/100‑1 (MQ=255) ggACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcg > 1:802680/1‑100 (MQ=255) gACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcgg > 1:690701/1‑100 (MQ=255) aCAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCCTCTTATTCACCGCCTGTTGATATTggcggc > 2:324761/1‑100 (MQ=255) cAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGgcggcg > 2:871450/1‑100 (MQ=255) cAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGgcggcg < 2:743429/100‑1 (MQ=255) aGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGt < 1:532891/100‑1 (MQ=255) ggTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg > 1:472424/1‑100 (MQ=255) gtTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATg < 2:745635/100‑1 (MQ=255) gtTACTGCGCTCAGTTTATTTGTCCCTGGACCCGGATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATg < 2:538469/100‑1 (MQ=255) tACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGt < 1:1294617/100‑1 (MQ=255) tACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGt > 2:218368/1‑100 (MQ=255) tACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGt > 2:1279874/1‑100 (MQ=255) aCTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTc < 1:577999/100‑1 (MQ=255) aCTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTc > 1:676118/1‑100 (MQ=255) cTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcg < 2:1077772/100‑1 (MQ=255) cTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcg > 2:158855/1‑100 (MQ=255) gcgcTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcggc > 2:1120463/1‑100 (MQ=255) | CTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGC > NC_000913/1519063‑1519254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |