| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,553,128 | T→C | V237V (GTA→GTG) | adhP ← | ethanol‑active dehydrogenase/acetaldehyde‑active reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,553,128 | 0 | T | C | 94.9% | 192.7 / 0.8 | 59 | V237V (GTA→GTG) | adhP | ethanol‑active dehydrogenase/acetaldehyde‑active reductase |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (29/27); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
GGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGC > NC_000913/1553031‑1553222 | ggATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCAc < 1:893435/100‑1 (MQ=255) atatCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCAcc < 2:809462/99‑1 (MQ=255) atCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGc > 2:413107/1‑99 (MQ=255) atCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCt > 1:649145/1‑100 (MQ=255) tCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGccc < 1:490704/100‑3 (MQ=255) tCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTg < 2:1046412/100‑1 (MQ=255) tCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTg > 1:462194/1‑100 (MQ=255) ccgatcttAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCGGCTTTAGCCACCGCTGTTa < 1:821569/93‑1 (MQ=255) aGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTa < 2:231057/100‑1 (MQ=255) aGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTa < 2:213985/100‑1 (MQ=255) ggCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTac > 1:737556/1‑100 (MQ=255) tCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTaccac < 2:1304206/100‑1 (MQ=255) cATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTaccacc > 2:562942/1‑100 (MQ=255) cATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTaccacc < 2:938829/100‑1 (MQ=255) tAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCGGCTTTAGCCACCGCTGTTaagatcgg > 2:821563/1‑93 (MQ=255) tAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGc > 1:987198/1‑100 (MQ=255) aGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACTGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCa < 2:1215648/100‑1 (MQ=255) cTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCg > 1:204597/1‑100 (MQ=255) cTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCg > 1:251653/1‑100 (MQ=255) cggTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAgcg < 1:44904/100‑1 (MQ=255) ccGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAg < 2:494299/100‑1 (MQ=255) ccGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGCTACCACCGCAGCGTGAGCGCCACCAg > 1:198877/1‑100 (MQ=255) aCAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGttt < 1:1144960/100‑1 (MQ=255) cAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGttt < 2:991378/99‑1 (MQ=255) aGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTtc < 2:737551/100‑1 (MQ=255) cacaACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTcc < 1:910850/100‑1 (MQ=255) cacaACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTcc < 2:959744/100‑1 (MQ=255) cacaACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTcc < 2:972846/100‑1 (MQ=255) acaacaCGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCt > 1:135082/1‑100 (MQ=255) caacaCGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCCCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCt > 2:1004739/1‑99 (MQ=255) acacGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGca > 2:1146038/1‑100 (MQ=255) cacGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGcac > 1:46790/1‑100 (MQ=255) gACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAAt < 2:400117/100‑1 (MQ=255) aCCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAAtt > 2:1204557/1‑100 (MQ=255) gCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTg < 1:1147030/100‑1 (MQ=255) ccTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTgg < 2:673016/100‑1 (MQ=255) cTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTggc > 1:538778/1‑100 (MQ=255) tGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTggcg > 1:969525/1‑100 (MQ=255) tGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTggcg > 1:1068859/1‑100 (MQ=255) gCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTggcgg > 2:1093900/1‑100 (MQ=255) cACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTggcggc < 1:355584/100‑1 (MQ=255) cACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTggcggc > 1:194224/1‑100 (MQ=255) cAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTc > 2:593678/1‑100 (MQ=255) gCATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCgg > 1:474510/1‑100 (MQ=255) cATCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGgt > 1:1022050/1‑100 (MQ=255) aTCAACTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGgtg < 1:952037/100‑1 (MQ=255) aCTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGtgtgtg < 1:853203/100‑1 (MQ=255) aCTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGtgtgtg < 1:1164343/100‑1 (MQ=255) cTGCCGAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGa > 2:501513/1‑100 (MQ=255) gAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTaa > 2:1066001/1‑100 (MQ=255) gAGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTaa > 2:586254/1‑100 (MQ=255) aGTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAAt > 1:107404/1‑100 (MQ=255) gTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATc > 1:117314/1‑100 (MQ=255) gTTAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATc < 1:738206/100‑1 (MQ=255) tAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGc > 1:497564/1‑100 (MQ=255) tAAACGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGc > 2:146559/1‑100 (MQ=255) cGCAGCTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTaaa > 1:273438/1‑100 (MQ=255) cTTTAGCCACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTgc < 2:987190/100‑1 (MQ=255) ttAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTgcgc > 1:101899/1‑100 (MQ=255) | GGATATCCAGGCTCATAGACTCCGGCGGTAGACCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGC > NC_000913/1553031‑1553222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |