| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 236,541 | C→T | R159* (CGA→TGA) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 236,541 | 0 | C | T | 95.2% | 133.2 / ‑1.5 | 42 | R159* (CGA→TGA) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (19/21); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
GTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATG > NC_000913/236445‑236633 | gTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaa > 2:112202/1‑100 (MQ=255) tCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaac > 2:617720/1‑100 (MQ=255) cACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAaatgaacg > 1:1206832/1‑100 (MQ=255) aTAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCa < 2:375389/100‑1 (MQ=255) gCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACgg > 1:1257226/1‑100 (MQ=255) gTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTAc > 2:594299/1‑100 (MQ=255) cGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGa > 2:1057751/1‑100 (MQ=255) aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGccc < 2:72707/100‑1 (MQ=255) aaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAg > 2:846916/1‑100 (MQ=255) cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTg < 1:171265/100‑1 (MQ=255) ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGc < 1:998124/100‑1 (MQ=255) cGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCg < 2:1089764/100‑1 (MQ=255) tAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGaa > 2:536624/1‑100 (MQ=255) aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGt > 1:507383/1‑100 (MQ=255) gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt > 2:500083/1‑100 (MQ=255) gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGAACGGGGCATTACGCGATGCCCAGATCCTTGCGGAAGttt > 1:296008/1‑99 (MQ=255) cAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTAt > 1:1245694/1‑100 (MQ=255) gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGc < 1:948045/100‑1 (MQ=255) cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGa < 2:836297/100‑1 (MQ=255) tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGAt > 2:460715/1‑100 (MQ=255) tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGAt < 2:370628/100‑1 (MQ=255) gCGTTATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCg > 1:157757/1‑100 (MQ=255) ttATGTGCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtg < 1:1067781/100‑1 (MQ=255) tgtgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTc < 2:278426/100‑1 (MQ=255) tgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa > 2:584509/1‑100 (MQ=255) tgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa < 2:144750/100‑1 (MQ=255) tgCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa < 1:1168487/100‑1 (MQ=255) gCTCGCTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaaa > 2:146439/1‑100 (MQ=255) gCTCGCTACGAAAAAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaaa > 2:804911/1‑100 (MQ=255) cTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg > 1:1080078/1‑100 (MQ=255) cTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg > 1:717853/1‑100 (MQ=255) cTACGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg > 1:20707/1‑100 (MQ=255) cGAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg < 1:536628/100‑1 (MQ=255) gAAATAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg < 1:346198/100‑1 (MQ=255) gAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCAAGATCCTCGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGCCGAt > 2:296848/1‑98 (MQ=255) aaTAGATAACAGTAAATGAACGCTTCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCt < 2:253612/100‑1 (MQ=255) aaTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCt > 2:274522/1‑100 (MQ=255) aaTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCt > 2:232089/1‑100 (MQ=255) aaTAGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGAGATGACCGGTGGTCAAACGTCGATGGCt > 1:515809/1‑100 (MQ=255) aGATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCtttt < 2:1197261/100‑1 (MQ=255) gATAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg < 1:397971/100‑1 (MQ=255) aTAACAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTgn < 2:992689/100‑2 (MQ=255) aCAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt < 1:112204/100‑1 (MQ=255) cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg < 1:935997/100‑1 (MQ=255) cAGTAAATGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg < 1:1272326/100‑1 (MQ=255) | GTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATG > NC_000913/236445‑236633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |