| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,778,470 | A→G | Y278H (TAC→CAC) | ydiP ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,778,470 | 0 | A | G | 98.4% | 227.8 / NA | 64 | Y278H (TAC→CAC) | ydiP | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (34/29); total (34/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATAA > NC_000913/1778371‑1778566 | aCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa > 2:278433/1‑100 (MQ=255) aaCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATc < 1:124892/100‑1 (MQ=255) cTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGc < 1:1207887/100‑1 (MQ=255) cTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGc > 1:853171/1‑100 (MQ=255) cgccgcCAGATTTATCCAACAAGACTTTCCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTgg < 1:450606/100‑1 (MQ=255) gccgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTggg < 2:1216562/100‑1 (MQ=255) ccgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGc > 1:845828/1‑100 (MQ=255) cgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCg > 2:823876/1‑100 (MQ=255) cgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCg > 2:355510/1‑100 (MQ=255) cgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCg > 1:13762/1‑100 (MQ=255) gccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCg < 2:999179/99‑1 (MQ=255) gccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGa > 1:221882/1‑100 (MQ=255) gcAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGaa > 2:237102/2‑100 (MQ=255) cAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGaa > 2:881845/1‑99 (MQ=255) tttATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGaacaacn < 1:278644/100‑2 (MQ=255) tttATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAAcc < 1:321740/100‑1 (MQ=255) tttATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAAcc > 1:423942/1‑100 (MQ=255) aTCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAAcc < 1:278436/97‑1 (MQ=255) aCTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCaaa > 1:615074/1‑100 (MQ=255) tACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAAc > 2:741568/1‑100 (MQ=255) tACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAAc > 2:159875/1‑100 (MQ=255) ggTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGtt < 2:1009671/100‑1 (MQ=255) ttGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTg < 2:1302781/100‑1 (MQ=255) tGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg < 2:1035891/100‑1 (MQ=255) tGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg > 1:280818/1‑100 (MQ=255) gAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg < 1:221213/99‑1 (MQ=255) ggAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCaa < 1:1105785/100‑1 (MQ=255) aaaTTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAg < 1:182751/100‑1 (MQ=255) aaaTTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGCTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAg > 2:1295348/1‑100 (MQ=255) gATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAACCAGTTTGACAAAGTGATCGa > 1:1253425/1‑100 (MQ=255) aTAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGAc > 1:230980/1‑100 (MQ=255) ggATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACAt > 1:1080917/1‑99 (MQ=255) ttCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCa < 2:515275/100‑1 (MQ=255) cTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATa > 2:504092/1‑100 (MQ=255) cTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATa > 1:861682/1‑100 (MQ=255) ttGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATaa > 2:1179041/1‑100 (MQ=255) gTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAcc > 1:413777/1‑100 (MQ=255) tCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCca > 2:236274/1‑100 (MQ=255) ccGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCcac > 2:587935/1‑100 (MQ=255) ccGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCcac < 2:413773/100‑1 (MQ=255) cGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCcaca < 2:221880/100‑1 (MQ=255) aaaCAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGcc > 2:1278297/1‑100 (MQ=255) aaaCAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGcc < 1:618400/100‑1 (MQ=255) aCAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAg < 1:1054603/100‑1 (MQ=255) cAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAgg > 2:66905/1‑100 (MQ=255) gTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCTAGgag < 1:454172/100‑1 (MQ=255) gTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgag < 2:230978/100‑1 (MQ=255) gTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgag > 2:888828/1‑100 (MQ=255) gTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgag < 1:261455/100‑1 (MQ=255) gTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgag < 2:571221/100‑1 (MQ=255) gTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgag > 2:601823/1‑100 (MQ=255) ttttAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGAt < 1:428003/100‑1 (MQ=255) tttAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGAtc > 1:803246/1‑100 (MQ=255) aaaTTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATctct > 1:484524/1‑100 (MQ=255) aaaTTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATctct > 1:944860/1‑100 (MQ=255) ttGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGcc < 1:2648/100‑1 (MQ=255) ttGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGcc < 2:405991/100‑1 (MQ=255) ttGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGcc < 2:13762/100‑1 (MQ=255) ttGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGcc < 1:741573/100‑1 (MQ=255) cTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCtgtg < 1:1278308/100‑1 (MQ=255) aCGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGAt > 1:419477/1‑100 (MQ=255) aCGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGAt > 2:338169/1‑100 (MQ=255) aCGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGAt > 1:1205680/1‑100 (MQ=255) cGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATa < 1:823882/100‑1 (MQ=255) ggTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATaa > 1:447144/1‑100 (MQ=255) | ACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATAA > NC_000913/1778371‑1778566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |