Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,983,761 | C→T | E437K (GAA→AAA) | araG ← | L‑arabinose ABC transporter ATPase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,983,761 | 0 | C | T | 98.3% | 193.4 / ‑4.1 | 58 | E437K (GAA→AAA) | araG | L‑arabinose ABC transporter ATPase |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (27/30); total (27/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 |
ATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCT > NC_000913/1983664‑1983856 | aTCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGt < 2:566901/100‑1 (MQ=255) cGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCt > 1:129720/1‑100 (MQ=255) ggCAACGCCGATGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCTCCGCCAGCGCATAACTTACGTTATATATTTTGTGCt < 2:641673/95‑1 (MQ=255) gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc < 1:355561/100‑1 (MQ=255) cAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGcc < 2:1296834/100‑1 (MQ=255) cGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAAc < 1:356051/100‑1 (MQ=255) cGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGAGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATc < 2:714732/100‑1 (MQ=255) aGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCaa < 2:660829/100‑1 (MQ=255) cTTCAGGTAAGTCGCTGGAGGCAAACATCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGcc < 2:1297266/100‑1 (MQ=255) cTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGcc > 2:322303/1‑100 (MQ=255) cTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGcc > 2:848206/1‑100 (MQ=255) ctggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcg < 2:209977/98‑1 (MQ=255) cAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcg > 1:985570/1‑100 (MQ=255) cAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcg > 1:278212/1‑100 (MQ=255) cAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcg > 2:486908/1‑100 (MQ=255) ggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcgcg < 1:226230/100‑1 (MQ=255) ggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcgag > 1:209979/1‑98 (MQ=255) gTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGt > 1:408756/1‑100 (MQ=255) tAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTa < 1:162060/100‑1 (MQ=255) aaGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAg < 2:222650/100‑1 (MQ=255) aGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAgg < 1:932303/100‑1 (MQ=255) gTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGt > 1:973616/1‑100 (MQ=255) ncGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGtt > 2:35961/2‑100 (MQ=255) gCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTCCGCTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTc > 1:280618/1‑99 (MQ=255) gCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTc < 2:233086/99‑1 (MQ=255) gCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCa > 2:572566/1‑100 (MQ=255) cTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCAt < 2:1212102/100‑1 (MQ=255) ggAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCAtag > 1:1212113/1‑98 (MQ=255) ggAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATcc < 1:53835/100‑1 (MQ=255) ggAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATcc > 1:782335/1‑100 (MQ=255) ggAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATcc > 2:430262/1‑100 (MQ=255) gAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCa > 2:112901/1‑100 (MQ=255) aGGCCAACAGCTCCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCTAACATCAATGCCGCGCGTAGGTTCACCCAg < 2:951840/100‑1 (MQ=255) aaaCAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaaa > 1:672595/1‑100 (MQ=255) aaCAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaaaa < 1:350032/100‑1 (MQ=255) aGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGa < 2:291396/100‑1 (MQ=255) cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAcc < 2:985562/100‑1 (MQ=255) cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATAGTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAcc < 1:690226/100‑1 (MQ=255) aCCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCt > 2:332789/1‑100 (MQ=255) ccgccccgccCTGCGCCGCCAGCGCATAAATTACGTTATATACTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGCCCt > 2:506031/1‑99 (MQ=255) ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt > 1:743582/1‑100 (MQ=255) ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCt > 2:450891/1‑99 (MQ=255) cGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATAGATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTc < 2:285071/100‑1 (MQ=255) cACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtc > 1:32594/1‑100 (MQ=255) cACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtc < 1:35962/100‑1 (MQ=255) cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc > 2:1301653/1‑100 (MQ=255) gccgccAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATa > 1:912662/1‑100 (MQ=255) cATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGcc < 2:1100969/100‑1 (MQ=255) aTAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGccc < 1:486912/100‑1 (MQ=255) aaaTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAg < 2:672591/100‑1 (MQ=255) aaaTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAg > 2:949009/1‑100 (MQ=255) cGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGc < 1:1181905/100‑1 (MQ=255) gTTATATATTTTGTGCTTAGCGCCCACATCAATGCCGCTCGTAAGTTCATCCAGCAAAATGCCCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGc > 2:637838/1‑99 (MQ=255) atatatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCtttt < 1:430266/100‑1 (MQ=255) tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc < 1:710233/100‑1 (MQ=255) tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc < 2:938776/100‑1 (MQ=255) tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc < 1:1014945/100‑1 (MQ=255) atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCGCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt > 1:1243226/1‑100 (MQ=255) | ATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCT > NC_000913/1983664‑1983856 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |