| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,988,339 | C→T | Q40* (CAG→TAG) | yecR → | lipoprotein, function unknown |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,988,339 | 0 | C | T | 98.2% | 186.1 / ‑4.4 | 55 | Q40* (CAG→TAG) | yecR | lipoprotein, function unknown |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (24/30); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTCCCC > NC_000913/1988247‑1988434 | cATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCttt > 2:1219667/1‑100 (MQ=255) actaataaCGCTGACGGGGTGTACGGTTACTAGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCtttt > 1:879698/3‑100 (MQ=255) ttAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCttttt < 2:1251660/100‑1 (MQ=255) ttAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCttttt > 2:489143/1‑100 (MQ=255) ttAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCttttt < 1:948298/100‑1 (MQ=255) taataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCtttttt > 2:1231721/1‑100 (MQ=255) taataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCtttttt < 1:243256/100‑1 (MQ=255) aataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTg > 2:597049/1‑100 (MQ=255) ataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgc < 2:932583/100‑1 (MQ=255) ataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgc > 1:1277044/1‑100 (MQ=255) taaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgca > 1:842407/1‑100 (MQ=255) aaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgcag < 2:582326/100‑1 (MQ=255) aaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgcag < 1:414367/100‑1 (MQ=255) aCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgcagc > 1:26992/1‑100 (MQ=255) aCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgcagc < 2:588531/100‑1 (MQ=255) cGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgcagca < 1:450592/100‑1 (MQ=255) ggCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGcc > 1:825002/1‑100 (MQ=255) ggCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGcc < 1:565531/100‑1 (MQ=255) cGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCa > 1:550337/1‑100 (MQ=255) cGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCa > 2:740832/1‑100 (MQ=255) cGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCa < 1:385911/100‑1 (MQ=255) ggggTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCAt < 2:201316/100‑1 (MQ=255) ggggTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCAt > 1:428294/1‑100 (MQ=255) gtgtACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATAcc < 1:876137/100‑1 (MQ=255) cGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCg < 1:702200/100‑1 (MQ=255) ttACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTa < 1:454905/100‑1 (MQ=255) ttACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTa < 1:268991/100‑1 (MQ=255) cGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCa > 1:1162468/1‑100 (MQ=255) ccAAGCCCATGTGAGCGAAGTTGACGCGGCAACATGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAg < 2:599994/99‑1 (MQ=255) gCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTcgcg < 2:845749/100‑1 (MQ=255) cccATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCgg > 1:165273/1‑100 (MQ=255) cccATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCgg < 2:386979/100‑1 (MQ=255) tgtgAGCGAAGTTGACGCGGCATCAGGTATTCTACGGCTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATc < 2:879691/100‑1 (MQ=255) gCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGa > 2:670185/1‑100 (MQ=255) cGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGAt > 1:299840/1‑100 (MQ=255) cGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTGTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCAGGATCGCCGAt < 2:54727/100‑1 (MQ=255) aGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATcgc < 1:740837/100‑1 (MQ=255) gTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATcgcg < 1:1152145/100‑1 (MQ=255) gTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATcgcg > 1:1062739/1‑100 (MQ=255) ttGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCgcgc > 2:658270/1‑100 (MQ=255) tGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGCGATCGGCGATCccgcg < 1:1231732/100‑2 (MQ=255) tGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATcgcg > 2:289455/1‑98 (MQ=255) aCGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATg < 1:662445/100‑1 (MQ=255) cAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCcagca < 1:1219678/100‑1 (MQ=255) aaCAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCcagcag < 1:40559/100‑1 (MQ=255) ggTATTGTACGGTTAGTTTATGACTAGGCTTTTTTTTTGTATTTTTATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATTCTAGCAGGAAg > 2:947020/1‑100 (MQ=255) ggTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAg > 1:298869/1‑100 (MQ=255) tATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGc > 2:1086840/1‑100 (MQ=255) ttGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCGCATCCCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCta < 1:715322/100‑1 (MQ=255) aCGGTTAGTTTATGACTAGGCTTTTTTGCAGCATACCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGc > 2:467591/1‑94 (MQ=255) ggTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATAGAAGTAGCAGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTCTAcaca > 2:1159438/1‑100 (MQ=255) aGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCAcgcg < 2:842401/100‑1 (MQ=255) gTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCgg > 1:452304/1‑100 (MQ=255) tATGACTAGGCTTTTTTGGAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTc < 2:482207/99‑1 (MQ=255) tGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTcccc < 1:597053/100‑1 (MQ=255) | CATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTCCCC > NC_000913/1988247‑1988434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |