Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,638	C→A	intergenic (+14/‑300)	asnT → / → yeeJ	tRNA‑Asn/putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,638	0	C	A	100.0%	190.7 / NA	53	intergenic (+14/‑300)	asnT/yeeJ	tRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/21);  total (32/21)

TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGT  >  NC_000913/2044549‑2044732                                                                                          |                                                                                               tCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAg                                                                                      >  2:591876/1‑100 (MQ=255)  ccTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAgc                                                                                     >  1:1275195/1‑100 (MQ=255)   ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAgcg                                                                                    <  1:812705/100‑1 (MQ=255)   ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAgcg                                                                                    >  1:944525/1‑100 (MQ=255)   ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAgcg                                                                                    >  1:1174711/1‑100 (MQ=255)     ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAgcgag                                                                                  >  2:812700/1‑98 (MQ=255)       ntAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAAt                                                                                >  1:431788/2‑100 (MQ=255)          gTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTc                                                                             >  1:1115189/1‑100 (MQ=255)           ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCa                                                                            >  1:953169/1‑100 (MQ=255)             cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACt                                                                          >  1:231989/1‑100 (MQ=255)               gTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGa                                                                        <  2:431784/100‑1 (MQ=255)                tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGAc                                                                       <  2:1151722/100‑1 (MQ=255)                  ggTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCt                                                                     >  2:818875/1‑100 (MQ=255)                     aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAg                                                                  >  2:448619/1‑100 (MQ=255)                     aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAg                                                                  >  1:342154/1‑100 (MQ=255)                      gAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGt                                                                 >  2:532350/1‑100 (MQ=255)                      gAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGt                                                                 <  1:912777/100‑1 (MQ=255)                                  gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttt                                                     >  1:1151075/1‑100 (MQ=255)                                    tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttttt                                                   <  1:1251114/100‑1 (MQ=255)                                     aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGTCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGtttttt                                                  >  2:925695/1‑100 (MQ=255)                                     aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGtttttt                                                  >  1:584352/1‑100 (MQ=255)                                     aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGtttttt                                                  >  2:330864/1‑100 (MQ=255)                                       tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTaa                                                <  1:520428/100‑1 (MQ=255)                                         cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACg                                              >  1:176574/1‑100 (MQ=255)                                              gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAg                                         <  1:887782/100‑1 (MQ=255)                                                 aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCgg                                      >  2:381925/1‑100 (MQ=255)                                                    ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCggg                                     >  1:853311/1‑98 (MQ=255)                                                    ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtt                                   >  1:511175/1‑100 (MQ=255)                                                      ttCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtttt                                 <  2:787707/100‑1 (MQ=255)                                                      ttCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtttt                                 >  2:556447/1‑100 (MQ=255)                                                      ttCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtttt                                 <  1:591880/100‑1 (MQ=255)                                                       tCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGttttt                                <  2:1115179/100‑1 (MQ=255)                                                        cGAGTCCAGTCAGAGGAGCCAAATTCTTAAAATTAGCTTTTTTAGCGCAATGTCACTGCCCTTCGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                               >  1:365878/1‑100 (MQ=39)                                                        cGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                               <  2:667231/100‑1 (MQ=255)                                                        cGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                               <  2:1273591/100‑1 (MQ=255)                                                         gAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTaa                              >  1:1267965/1‑100 (MQ=255)                                                           gTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACa                            >  1:653898/1‑100 (MQ=255)                                                           gTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACa                            <  1:1265387/100‑1 (MQ=255)                                                              cAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCt                         <  1:495282/100‑1 (MQ=255)                                                               aGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCtt                        <  2:825156/100‑1 (MQ=255)                                                                gTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTa                       <  1:1068591/100‑1 (MQ=255)                                                                  cAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAg                     <  1:3262/100‑1 (MQ=255)                                                                    gaggagCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgcg                   >  1:457661/1‑100 (MQ=255)                                                                    gaggagCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgcg                   >  1:530839/1‑100 (MQ=255)                                                                          ccAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCg             >  2:702762/1‑100 (MQ=255)                                                                           cAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGa            >  1:84308/1‑100 (MQ=255)                                                                              aTTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCt         <  2:1155371/100‑1 (MQ=255)                                                                               ttCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCtt        <  2:451886/100‑1 (MQ=255)                                                                                 cTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTa      >  1:1260456/1‑100 (MQ=255)                                                                                  tAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTAt     >  2:1049768/1‑100 (MQ=255)                                                                                   aaaaaTTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATg    >  1:313774/1‑100 (MQ=255)                                                                                    aaaaTTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATgg   <  2:511171/100‑1 (MQ=255)                                                                                     aaaTTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGt  <  1:144484/100‑1 (MQ=255)                                                                                          |                                                                                               TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGT  >  NC_000913/2044549‑2044732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑